Long non-coding RNA BCAR4 is required for efficient influenza A virus replication

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

Abstract Long non-coding RNAs (lncRNAs) regulate diverse biological processes, including influenza A virus (IAV) infection. However, the understanding of lncRNAs in IAV infection is limited. By using both bioinformatic analyses and virological assays, we showed that lncRNA BCAR4 expression can be highly induced by multiple different subtypes. was required for propagation Genetic inactivation inhibited growth. Investigation cycle revealed a suppressed viral RNA transcription replication, attenuated protein synthesis -deficient cells. potentially interacted with cellular splicing-associated proteins activation associated NS segment. These findings suggest important role regulation Importance serve as critical regulators processes how engage remains unclear. Here show universally Deletion reduced multiplication. In cycle, deficiency decreased transcription, replication biosynthesis. binding to host splicing protein, segment . Our results highlight

Язык: Английский

Human long noncoding RNA VILMIR is induced by major respiratory viral infections and modulates the host interferon response

Journal of Virology, Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

ABSTRACT Long noncoding RNAs (lncRNAs) are a newer class of transcripts identified as key regulators biological processes. Here, we aimed to identify novel lncRNA targets that play critical roles in major human respiratory viral infections by systematically mining large-scale transcriptomic data sets. Using bulk RNA-sequencing (RNA-seq) analysis, previously uncharacterized lncRNA, named virus-inducible modulator interferon response ( VILMIR ), was consistently upregulated after vitro influenza infection across multiple epithelial cell lines and A virus subtypes. also severe acute syndrome coronavirus 2 (SARS-CoV-2) syncytial (RSV) . We experimentally confirmed the interferon-beta (IFN-β) treatment A549 line found expression robustly induced IFN-β dose- time-specific manner. Single-cell RNA-seq analysis bronchoalveolar lavage fluid samples from disease 2019 (COVID-19) patients uncovered various types, including at least five immune cells. The upregulation cells further T monocyte lines, SUP-T1 THP-1, treatment. Finally, knockdown reduced magnitude host transcriptional responses both Together, our results show is interferon-stimulated gene (ISG) regulates may be potential therapeutic target for upon mechanistic investigation. IMPORTANCE Identifying factors regulate developing new therapeutics. Human long have been regulatory during processes; however, majority functions within antiviral remain unknown. In this study, influenza, 2, virus. demonstrated an several types. Our reveal present infections.

Язык: Английский

Positive Regulation of Cellular Proteins by Influenza Virus for Productive Infection

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3584 - 3584

Опубликована: Апрель 10, 2025

Influenza viruses cause annual epidemics and occasional pandemics through respiratory tract infections, giving rise to substantial morbidity mortality worldwide. extensively interact with host cellular proteins exploit a variety of pathways accomplish their infection cycle. Some the that display negative effects on virus are degraded by virus. However, there also various upregulated influenza at expression and/or activation levels. It has been well-established large number antiviral such as type I interferon-stimulated genes elevated viral infection. On other hand, many induced directly virus, which considered pro-viral factors often indispensable for rigorous propagation or pathogenicity. Here, we review recent advances in our understanding deemed be utilized The focus is placed functions these mechanisms associated promoting amplification, evading immunity, enhancing Investigating process how hijack could provide framework inventing host-factor-targeted drugs conquer influenza.

Язык: Английский