Differential Gene Expression Analysis in a Lumbar Spinal Stenosis Rat Model via RNA Sequencing: Identification of Key Molecular Pathways and Therapeutic Insights
Biomedicines,
Год журнала:
2025,
Номер
13(1), С. 192 - 192
Опубликована: Янв. 14, 2025
Lumbar
spinal
stenosis
(LSS)
is
a
degenerative
condition
characterized
by
the
narrowing
of
canal,
resulting
in
chronic
pain
and
impaired
mobility.
However,
molecular
mechanisms
underlying
LSS
remain
unclear.
In
this
study,
we
performed
RNA
sequencing
(RNA-seq)
to
investigate
differential
gene
expression
rat
model
identify
key
genes
pathways
involved
its
pathogenesis.
We
used
bioinformatics
analysis
significant
alterations
between
LSS-induced
sham
groups.
Pearson's
correlation
demonstrated
strongly
consistent
intragroup
(r
>
0.9),
with
distinct
A
total
113
differentially
expressed
(DEGs)
were
identified,
including
upregulated
such
as
Slc47a1
Prg4
downregulated
Higd1c
Mln.
Functional
enrichment
revealed
that
these
DEGs
included
those
biological
processes,
synaptic
plasticity,
extracellular
matrix
organization,
hormonal
regulation.
Gene
ontology
highlighted
critical
functions
mRNA
binding
integrin
binding,
well
cellular
components
contractile
fibers
matrix,
which
significantly
affected
LSS.
Our
findings
provide
novel
insights
into
offer
potential
avenues
for
development
targeted
therapies
aimed
at
mitigating
disease
progression
improving
patient
outcomes.
Язык: Английский
Sdc4 deletion perturbs intervertebral disc matrix homeostasis and promotes early osteopenia in the aging mouse spine
Matrix Biology,
Год журнала:
2024,
Номер
131, С. 46 - 61
Опубликована: Май 26, 2024
Язык: Английский
Protective Effects of Vitamin D on Proteoglycans of Human Articular Chondrocytes through TGF-β1 Signaling
Nutrients,
Год журнала:
2024,
Номер
16(17), С. 2991 - 2991
Опубликована: Сен. 4, 2024
The
extracellular
matrix
of
cartilage
primarily
constitutes
collagen
and
aggrecan.
Cartilage
degradation
starts
with
aggrecan
loss
in
osteoarthritis
(OA).
Vitamin
D
(VD)
plays
an
essential
role
several
inflammation-related
diseases
can
protect
the
during
OA.
present
study
focused
on
VD
turnover
human
articular
chondrocytes
treated
tumor
necrosis
factor
α
(TNF-α)
possible
mechanism.
Treatment
different
doses
periods
intervention
TNF-α
TGF-β1
receptor
(TGFβR1)
inhibitor
SB525334
were
investigated.
viability
secretion
measured.
expression
intracellular
TGFβR1
was
examined.
Transcriptional
translational
levels
related
metabolic
factors
analyzed.
results
showed
that
markedly
reduced
viability,
expressions
chondrocytes,
increased
disintegrin
metalloproteinase
thrombospondin
motifs.
alterations
partially
inhibited
by
treatment.
Furthermore,
effects
blocked
TNF-α-treated
cells.
may
prevent
proteoglycan
due
to
via
signaling
chondrocytes.
Язык: Английский
Proteoglycan Dysfunction as a Key Hallmark of Intervertebral Disc Degeneration: Commentary on “Proteoglycan Dysfunction: A Common Link Between Intervertebral Disc Degeneration and Skeletal Dysplasia”
Neurospine,
Год журнала:
2024,
Номер
21(1), С. 179 - 181
Опубликована: Март 28, 2024
See
the
article
"Proteoglycan
Dysfunction:
A
Common
Link
Between
Intervertebral
Disc
Degeneration
and
Skeletal
Dysplasia"
in
Volume
21
on
page
162.
Язык: Английский
Biomimetic strategies for the deputization of proteoglycan functions
Frontiers in Cell and Developmental Biology,
Год журнала:
2024,
Номер
12
Опубликована: Авг. 6, 2024
Proteoglycans
(PGs),
which
have
glycosaminoglycan
chains
attached
to
their
protein
cores,
are
essential
for
maintaining
the
morphology
and
function
of
healthy
body
tissues.
Extracellular
PGs
perform
various
functions,
classified
into
following
four
categories:
i)
modulation
tissue
mechanical
properties;
ii)
regulation
protection
extracellular
matrix;
iii)
sequestration;
iv)
cell
signaling.
The
depletion
may
significantly
impair
function,
encompassing
compromised
characteristics
unregulated
inflammatory
responses.
Since
play
critical
roles
in
tissues
synthesis
is
complex,
development
PG
mimetic
molecules
that
recapitulate
functions
engineering
therapeutic
applications
has
attracted
interest
researchers
more
than
20
years.
These
approaches
ranged
from
semisynthetic
graft
copolymers
recombinant
domains
produced
by
cells
undergone
genetic
modifications.
This
review
discusses
some
mimicking
these
functions.
Язык: Английский