Biomimetic strategies for the deputization of proteoglycan functions DOI Creative Commons
Ibrahim F. Rehan,

Asmaa Elnagar,

František Zigo

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 12

Опубликована: Авг. 6, 2024

Proteoglycans (PGs), which have glycosaminoglycan chains attached to their protein cores, are essential for maintaining the morphology and function of healthy body tissues. Extracellular PGs perform various functions, classified into following four categories: i) modulation tissue mechanical properties; ii) regulation protection extracellular matrix; iii) sequestration; iv) cell signaling. The depletion may significantly impair function, encompassing compromised characteristics unregulated inflammatory responses. Since play critical roles in tissues synthesis is complex, development PG mimetic molecules that recapitulate functions engineering therapeutic applications has attracted interest researchers more than 20 years. These approaches ranged from semisynthetic graft copolymers recombinant domains produced by cells undergone genetic modifications. This review discusses some mimicking these functions.

Язык: Английский

Differential Gene Expression Analysis in a Lumbar Spinal Stenosis Rat Model via RNA Sequencing: Identification of Key Molecular Pathways and Therapeutic Insights DOI Creative Commons
Jin Young Hong, Wan-Jin Jeon,

Hyunseong Kim

и другие.

Biomedicines, Год журнала: 2025, Номер 13(1), С. 192 - 192

Опубликована: Янв. 14, 2025

Lumbar spinal stenosis (LSS) is a degenerative condition characterized by the narrowing of canal, resulting in chronic pain and impaired mobility. However, molecular mechanisms underlying LSS remain unclear. In this study, we performed RNA sequencing (RNA-seq) to investigate differential gene expression rat model identify key genes pathways involved its pathogenesis. We used bioinformatics analysis significant alterations between LSS-induced sham groups. Pearson's correlation demonstrated strongly consistent intragroup (r > 0.9), with distinct A total 113 differentially expressed (DEGs) were identified, including upregulated such as Slc47a1 Prg4 downregulated Higd1c Mln. Functional enrichment revealed that these DEGs included those biological processes, synaptic plasticity, extracellular matrix organization, hormonal regulation. Gene ontology highlighted critical functions mRNA binding integrin binding, well cellular components contractile fibers matrix, which significantly affected LSS. Our findings provide novel insights into offer potential avenues for development targeted therapies aimed at mitigating disease progression improving patient outcomes.

Язык: Английский

Процитировано

0

Sdc4 deletion perturbs intervertebral disc matrix homeostasis and promotes early osteopenia in the aging mouse spine DOI
Kimheak Sao, Makarand V. Risbud

Matrix Biology, Год журнала: 2024, Номер 131, С. 46 - 61

Опубликована: Май 26, 2024

Язык: Английский

Процитировано

2

Protective Effects of Vitamin D on Proteoglycans of Human Articular Chondrocytes through TGF-β1 Signaling DOI Open Access
Jian Guan, Zhuoxin Li, Guodong Niu

и другие.

Nutrients, Год журнала: 2024, Номер 16(17), С. 2991 - 2991

Опубликована: Сен. 4, 2024

The extracellular matrix of cartilage primarily constitutes collagen and aggrecan. Cartilage degradation starts with aggrecan loss in osteoarthritis (OA). Vitamin D (VD) plays an essential role several inflammation-related diseases can protect the during OA. present study focused on VD turnover human articular chondrocytes treated tumor necrosis factor α (TNF-α) possible mechanism. Treatment different doses periods intervention TNF-α TGF-β1 receptor (TGFβR1) inhibitor SB525334 were investigated. viability secretion measured. expression intracellular TGFβR1 was examined. Transcriptional translational levels related metabolic factors analyzed. results showed that markedly reduced viability, expressions chondrocytes, increased disintegrin metalloproteinase thrombospondin motifs. alterations partially inhibited by treatment. Furthermore, effects blocked TNF-α-treated cells. may prevent proteoglycan due to via signaling chondrocytes.

Язык: Английский

Процитировано

2

Proteoglycan Dysfunction as a Key Hallmark of Intervertebral Disc Degeneration: Commentary on “Proteoglycan Dysfunction: A Common Link Between Intervertebral Disc Degeneration and Skeletal Dysplasia” DOI Creative Commons
Takashi Yurube

Neurospine, Год журнала: 2024, Номер 21(1), С. 179 - 181

Опубликована: Март 28, 2024

See the article "Proteoglycan Dysfunction: A Common Link Between Intervertebral Disc Degeneration and Skeletal Dysplasia" in Volume 21 on page 162.

Язык: Английский

Процитировано

0

Biomimetic strategies for the deputization of proteoglycan functions DOI Creative Commons
Ibrahim F. Rehan,

Asmaa Elnagar,

František Zigo

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 12

Опубликована: Авг. 6, 2024

Proteoglycans (PGs), which have glycosaminoglycan chains attached to their protein cores, are essential for maintaining the morphology and function of healthy body tissues. Extracellular PGs perform various functions, classified into following four categories: i) modulation tissue mechanical properties; ii) regulation protection extracellular matrix; iii) sequestration; iv) cell signaling. The depletion may significantly impair function, encompassing compromised characteristics unregulated inflammatory responses. Since play critical roles in tissues synthesis is complex, development PG mimetic molecules that recapitulate functions engineering therapeutic applications has attracted interest researchers more than 20 years. These approaches ranged from semisynthetic graft copolymers recombinant domains produced by cells undergone genetic modifications. This review discusses some mimicking these functions.

Язык: Английский

Процитировано

0