Biological Function Analysis of MicroRNAs and Proteins in the Cerebrospinal Fluid of Patients with Parkinson’s Disease

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13260 - 13260

Опубликована: Дек. 10, 2024

Parkinson's disease (PD) is a progressive neurodegenerative disorder characterized by alpha-synuclein aggregation into Lewy bodies in the neurons. Cerebrospinal fluid (CSF) considered most suited source for investigating PD pathogenesis and identifying biomarkers. While microRNA (miRNA) profiling can aid investigation of post-transcriptional regulation diseases, information on miRNAs CSF patients with remains limited. This review combines miRNA analysis proteomic to explore collective impact mechanisms PD. We constructed separate networks altered proteomes using bioinformatics method. Altered were poorly linked biological functions owing limited information; however, changes protein expression strongly associated functions. Subsequently, integrated further analysis. In silico prediction from network revealed relationships between proteins, highlighting increased reactive oxygen species generation, neuronal loss, neurodegeneration suppressed ATP synthesis, mitochondrial function, neurotransmitter release The approach suggests potential as biomarkers critical underlying combined strategy could enhance our understanding complex biochemical support development diagnostic therapeutic strategies precision medicine.

Язык: Английский

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Differences in Blood and Cerebrospinal Fluid Between Parkinson’s Disease and Related Diseases

Cellular and Molecular Neurobiology, Год журнала: 2024, Номер 45(1)

Опубликована: Дек. 27, 2024

It is difficult to distinguish Parkinson's disease (PD) in the early stage from those of various disorders including atypical syndrome (APS), vascular parkinsonism (VP), and even essential tremor (ET), because overlap symptoms. Other, more challenging problems will arise when develops into dementia (PDD) middle late stages. At this time, differential diagnosis PDD DLB becomes thorny. These complicate diagnostic process for PD, which traditionally heavily relies on symptomatic assessment treatment response. Recent advances have identified several biomarkers blood cerebrospinal fluid (CSF), α-synuclein, lysosomal enzymes, fatty acid-binding proteins, neurofilament light chain, whose concentration differs PD related diseases. However, not all these molecules can effectively discriminate disorders. This review advocates a paradigm shift toward biomarker-based between similar conditions. may reflect diversity that exist among different diseases provide an effective way accurately understand their mechanisms. focused CSF value molecular measurement methods with high performance due emerging technologies.

Язык: Английский

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Neurological Disorders and Challenges in Their Theranostics

Опубликована: Янв. 1, 2023

Neurological disorders encompass multifaceted and heterogeneous ailments that influence the whole body though central peripheral nervous systems. These conditions give rise to a variety of incapacitating symptoms, thereby exerting an important impact on overall well-being countless individuals across globe. This book chapter provides overview complex realm neurological formidable obstacles they present within framework theranostics, with objective tailoring personalized treatment. The foremost common will be covered, including but not limited Alzheimer's disease, Parkinson's multiple sclerosis, epilepsy, accentuating their clinical pathophysiology profound onus impose upon patients, carers, healthcare For timely identification disease onset precise categorization, diagnostic methodologies included, encompassing neuroimaging, cerebrospinal fluid biomarkers, genetic profiling. However, encountered in development efficacious theranostic strategies for also mentioned (e.g., drug delivery blood-brain barrier). elucidation emerging technologies, nanomedicine, gene therapy, precision medicine, presents promising avenues surmounting these challenges heralding novel epoch therapeutics. By establishing connection between diagnosis treatment, researchers clinicians can strive offer more effective interventions address nature disorders.

Язык: Английский

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Rasagiline Confers Neuroprotection to PC12 Cell Cultures Exposed to Oxygen-Glucose Deprivation/Reoxygenation Insult by Activating the Akt/Nrf2 Redox-Signaling Pathway and by Decreasing the Nuclear Translocation of Glyceraldehyde-3-Phosphate Dehydroge

Опубликована: Июль 1, 2024

Rasagiline (Azilect®) is a selective monoamine oxidase B (MAO-B) inhibitor that provides symp-tomatic benefit in Parkinson’s disease (PD) treatment and found to exert preclinical neuroprotec-tive effects. Here, we investigated the neuroprotective signaling pathways of rasagiline PC12 neuronal cultures exposed an ischemic-like insult. Exposure neurons oxy-gen-glucose deprivation for 4 h followed by 18 reoxygenation caused 40% aponecrotic cell death. induced dose-dependent neuroprotection decreasing death produc-tion neurotoxic reactive oxygen species, reducing nuclear translocation glycer-aldehyde-3-phosphate dehydrogenase (GAPDH). increased protein kinase (Akt) phosphorylation decreased expression ischemia-induced α-synuclein protein, correlation effect. Treatment with shuttling transcription factor Nrf2 mRNA levels antioxidant enzymes heme oxy-genase-1, (NAD (P) H- quinone dehydrogenase, catalase. These results indicate ischemic inhibition GAPDH-mediated death, via mitochondrial protection, as increasing mi-tochondria-specific mechanism involving Akt/Nrf2 redox-signaling pathway. findings may be exploited drug development PD stroke therapy.

Язык: Английский

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Rasagiline Exerts Neuroprotection towards Oxygen–Glucose-Deprivation/Reoxygenation-Induced GAPDH-Mediated Cell Death by Activating Akt/Nrf2 Signaling

Biomedicines, Год журнала: 2024, Номер 12(7), С. 1592 - 1592

Опубликована: Июль 17, 2024

Rasagiline (Azilect

Язык: Английский

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Vascular Contributions to Healthy Aging and Dementia

Aging and Disease, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Vascular pathologies are among the most common contributors to neurodegenerative changes across spectrum of normal aging dementia. Cerebral small vessel disease (SVD) encompasses a wide range conditions affecting capillaries, arteries, and arterioles, as well perivascular spaces fluid dynamics in brain, playing significant role vascular contributions cognitive impairment dementia (VCID). These factors can accelerate progression SVD neuronal degeneration. Since is primary risk factor for Alzheimer's (AD) AD-related dementias (ADRD), this Research Topic aims gather recent research better understand healthy age-related impairment. Other include diabetes, lifestyle factors, high cholesterol, inflammation, immune remodeling, all which dysfunction progression. This special issue includes total 21 articles comprising Reviews, Perspectives, Original articles. The cover various technical biological aspects related progress research. We aim promote exchange different fields, including imaging, VCID, molecular biology, neuroinflammation, immunology. Most papers focus on understanding mechanisms AD/ADRD developing new therapeutic strategies.

Язык: Английский

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Blood and cerebrospinal fluid differences between Parkinson's disease and related diseases

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 27, 2024

Accurately diagnosing Parkinson’s disease (PD) in its early stages is difficult due to symptoms overlapping with those of various disorders, including atypical Parkinsonian syndromes, dementia Lewy bodies (DLB), and even essential tremor. This complicates the diagnostic process for PD, which traditionally heavily relies on symptomatic assessment treatment response. Recent advances have identified several biomarkers blood cerebrospinal fluid (CSF), α-synuclein, lysosomal enzymes, fatty acid-binding proteins, neurofilament light chain, that may potentially be used diagnosed PD. However, not all can effectively distinguish PD from related disorders or identify subtypes. review advocates a paradigm shift towards biomarker-based diagnosis between similar conditions categorize into These reflect differences exist among different diseases provide an effective way accurately understand their mechanisms. focused CSF differential value molecular measurement methods high performance emerging technologies.

Язык: Английский

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Exploring and Validating Key Genetic Biomarkers for Diagnosis of Parkinson's Disease

Brain Research Bulletin, Год журнала: 2024, Номер unknown, С. 111165 - 111165

Опубликована: Дек. 1, 2024

Parkinson's disease (PD) is a neurological condition characterized by complex genetic basic, and the reliable diagnosis of PD remained limited.

Язык: Английский

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