Clinical efficacy and pharmacological mechanism of Er Zhi Tian Gui formula in enhancing IVF-ET outcomes for diminished ovarian reserve DOI Creative Commons
Wen Chen, Fang Lian, Jing‐Yan Song

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Март 21, 2025

Objective To investigate the efficacy of Er Zhi Tian Gui Formula (EZTGF) in diminished ovarian reserve (DOR) patients undergoing vitro fertilization-embryo transfer (IVF-ET); to examine pharmacological mechanism EZTGF inhibiting granulosa cell (OGC) apoptosis. Methods A total 120 DOR IVF-ET Affiliated Hospital Shandong University TCM from March December 2021 were randomly assigned (n = 60) or Placebo groups. All participants followed a gonadotropin-releasing hormone antagonist protocol for controlled stimulation, with interventions starting on days 2–3 preceding menstrual cycle and continuing until trigger day. outcomes measured included number oocytes retrieved, embryo quality, pregnancy rates. NEAT1, miR-10b-3p, FOXO3a OGCs expression was analyzed via qRT-PCR. Mechanistic studies conducted using KGN cells, dual-luciferase reporter assays confirming regulatory relationship between FOXO3a. Results 116 completed trial (58 each group). The group showed significant increase both (especially at cleavage stage) rate high-quality embryos, compared (P < 0.05). No statistically differences observed > Cellular experiments that miR-10b-3p inhibits proliferation promotes apoptosis oocyte cells (OGCs). Dual luciferase gene assay confirms target-regulatory NEAT1 treatment partially reversed low FOXO3a, high patients. Conclusion Treatment enhances quality women IVF-ET, which can be attributed modulation NEAT1/miR-10b-3p/FOXO3a pathway. Trial registration: ChiCTR2100052522.

Язык: Английский

Clinical efficacy and pharmacological mechanism of Er Zhi Tian Gui formula in enhancing IVF-ET outcomes for diminished ovarian reserve DOI Creative Commons
Wen Chen, Fang Lian, Jing‐Yan Song

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Март 21, 2025

Objective To investigate the efficacy of Er Zhi Tian Gui Formula (EZTGF) in diminished ovarian reserve (DOR) patients undergoing vitro fertilization-embryo transfer (IVF-ET); to examine pharmacological mechanism EZTGF inhibiting granulosa cell (OGC) apoptosis. Methods A total 120 DOR IVF-ET Affiliated Hospital Shandong University TCM from March December 2021 were randomly assigned (n = 60) or Placebo groups. All participants followed a gonadotropin-releasing hormone antagonist protocol for controlled stimulation, with interventions starting on days 2–3 preceding menstrual cycle and continuing until trigger day. outcomes measured included number oocytes retrieved, embryo quality, pregnancy rates. NEAT1, miR-10b-3p, FOXO3a OGCs expression was analyzed via qRT-PCR. Mechanistic studies conducted using KGN cells, dual-luciferase reporter assays confirming regulatory relationship between FOXO3a. Results 116 completed trial (58 each group). The group showed significant increase both (especially at cleavage stage) rate high-quality embryos, compared (P < 0.05). No statistically differences observed > Cellular experiments that miR-10b-3p inhibits proliferation promotes apoptosis oocyte cells (OGCs). Dual luciferase gene assay confirms target-regulatory NEAT1 treatment partially reversed low FOXO3a, high patients. Conclusion Treatment enhances quality women IVF-ET, which can be attributed modulation NEAT1/miR-10b-3p/FOXO3a pathway. Trial registration: ChiCTR2100052522.

Язык: Английский

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