Novel Sensitive Electrochemical Immunosensor Development for the Selective Detection of HopQ H. pylori Bacteria Biomarker

Biosensors, Год журнала: 2023, Номер 13(5), С. 527 - 527

Опубликована: Май 8, 2023

Helicobacter pylori (H. pylori) is a highly contagious pathogenic bacterium that can cause gastrointestinal ulcers and may gradually lead to gastric cancer. H. expresses the outer membrane HopQ protein at earliest stages of infection. Therefore, reliable candidate as biomarker for detection in saliva samples. In this work, an immunosensor based on detecting saliva. The was developed by surface modification screen-printed carbon electrodes (SPCE) with MWCNT-COOH decorated gold nanoparticles (AuNP) followed capture antibody grafting SPCE/MWCNT/AuNP using EDC/S-NHS chemistry. sensor performance investigated utilizing various methods, such cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscope (SEM) coupled energy-dispersive X-ray (EDX). spiked samples evaluated square wave (SWV). suitable excellent sensitivity linearity 10 pg/mL-100 ng/mL range, 2.0 pg/mL limit (LOD) 8.6 quantification (LOQ). tested ng/mL, recovery 107.6% obtained SWV. From Hill's model, dissociation constant Kd HopQ/HopQ interaction estimated be 4.60 × 10-10 mg/mL. fabricated platform shows high selectivity, good stability, reproducibility, cost-effectiveness early due proper choice biomarker, nanocomposite material utilization boost SPCE electrical performance, intrinsic selectivity antibody-antigen approach. Additionally, we provide insight into possible future aspects researchers are recommended focus on.

Язык: Английский

---

Effects of miR-145-5p on cardiomyocyte proliferation and apoptosis, GIGYF1 expression and oxidative stress response in rats with myocardial ischemia-reperfusion

Cellular and Molecular Biology, Год журнала: 2022, Номер 68(1), С. 147 - 159

Опубликована: Май 22, 2022

This study aimed to investigate the effects of miR-145-5p on cardiomyocyte proliferation and apoptosis, GIGYF1 expression, inflammation, oxidative stress in rats with myocardial ischemia-reperfusion injury (IRI). For this purpose, SPF male SD were used for IRI modeling. Experimental animals subjected specimen sampling HE staining. The relative expression was detected by qRT-PCR; protein expressions GIGYF1, p-AKT, p53, Bax, p38MAPK, ERK1/2 Western blot. Mouse embryonic cardiomyocytes H9C2 H/R modeling, which then cell transfection according different grouping protocols. target confirmed be dual-luciferase reporter assay. Further experiments performed detect survival rate transfected cells, apoptosis SOD activity determination, as well IL-1β IL-6 concentrations. results showed that level downregulated H2C2 cells (P < 0.05). After 24h transfection, there a significant increase H/R+miR-145-5p mimic group 0.05), but an evident decrease inhibitor > Compared H/R+NC group, had significantly increased proliferation, improved release SOD, upregulated p-AKT; concentrations IL-6, decreased P38MAPK, Bax (all P Also, IRI+miR-145-5p agomir degree heart tissue improved; Dual-luciferase assay identified gene miR-145-5p. Furthermore, through stimulated overexpression miR-145-5p, while above trends reversed following simultaneous upregulation In general, our or model vivo vitro. Overexpression can downregulate further promote inhibit alleviate inflammation stress, hence exert protective role infarction IRI.

Язык: Английский

---

The role of circRNA polyribonucleotide nucleoside transferase 1 on gestational diabetes mellitus

Cellular and Molecular Biology, Год журнала: 2022, Номер 68(6), С. 148 - 154

Опубликована: Июнь 30, 2022

This study aimed to focus on the mechanism of circRNA polyribonucleotide nucleoside transferase 1 (circ-PNPT1)-mediated miR-889-3p/PAK1 gestational diabetes mellitus (GDM). Placental tissues from normal pregnancy and GDM patients were collected detect levels circ-PNPT1, miR-889-3p, PAK1. The high glucose-induced human trophoblast cells HTR-8/SVneo adopted stimulate model in vitro (HG group) transfected with lentivirus silence circ-PNPT1 (si-circ-PNPT1 mimic overexpress miR-889-3p (miR-889-3p group). Cell proliferation, apoptosis, migration, invasion detected by CKK-8, flow cytometry, Transwell, scratch assay, respectively. results showed that expressions PAK1 up-regulated, was down-regulated (P< 0.05). Compared control group, HG group cell abilities weakened, apoptosis rate increased E-cadherin protein elevated, N-cadherin Vimentin decreased other two groups decreased, enhanced, E-cadherin, N-cadherin, There targeted binding sites for PAK1, indicating promoted HG-induced dysfunction through axis.

Язык: Английский

---

Effect of Solanum lyratum Polysaccharide on Malignant Behaviors of Lung Cancer Cells by Regulating the Circ_UHRF1/miR-513b-5p Axis

Cellular and Molecular Biology, Год журнала: 2022, Номер 67(6), С. 191 - 199

Опубликована: Фев. 27, 2022

This study aimed to investigate the effect of Solanum lyratum polysaccharide on malignant behavior lung cancer cells and its possible mechanism. For this purpose, A549 were cultured in vitro treated with different doses (0.4, 0.8, 1.2 mg/mL) for 24 h. Then cell proliferation was detected by CCK-8 method clone formation test. Transwell test used detect migration invasion, flow cytometry apoptosis. The protein expressions Bax Bcl-2 Western blotting, circ_UHRF1 miR-513b-5p qRT-PCR method. Pearson correlation analyze between tissues. Results showed that compared control group, inhibition rate apoptosis intervened expression all increased (P<0.05), but number clones, invasion decreased dose-dependent. S. (P<0.05). tissues higher than adjacent lower negatively correlated (r=-0.861, P<0.05). Circ_UHRF1 could target miR-513b-5p, knocking down increased. After circ_UHRF1, Up-regulation reduced effects proliferation, migration, cells. In general, inhibit cells, induce Its mechanism may be related regulation circ_UHRF1/miR-513b-5p axis.

Язык: Английский

---

MiR-140 targets Wnt1 to inhibit the proliferation and enhance drug sensitivity in osteosarcoma cells

Cellular and Molecular Biology, Год журнала: 2022, Номер 68(1), С. 140 - 146

Опубликована: Май 22, 2022

MicroRNAs (miRNAs) have been documented to function differently in numerous human cancers. Our study planned investigate the role of microRNA-140 (miR-140) and identify its possible target osteosarcoma (OS) predict their mechanism OS. The miR-140 was down-regulated OS, high expression decreased MG63 cell proliferation. At molecular level, Wnt1 a miR-140, could be suppressed by miR-140. Besides, overexpression drug resistance OS cells treated doxorubicin. Collectively, may inhibit proliferation enhance sensitivity direct regulation Wnt/β-catenin signaling.

Язык: Английский

---

Helicobacter pylori infection in Africa: comprehensive insight into its pathogenesis, management, and future perspectives

Journal of Umm Al-Qura University for Applied Sciences, Год журнала: 2024, Номер unknown

Опубликована: Май 30, 2024

Abstract Helicobacter pylori is a widespread bacterium that has effectively colonized half of the global population, with Africa having over 70% total burden H. infections (HPI). Considering its acknowledged classification as bacterial carcinogens and their significant contribution to development gastrointestinal disorders such gastritis, peptic ulcers, gastric neoplasia, together growing resistance antibiotics. Gaining insight into etiology this organism crucial in order investigate develop appropriate treatment strategies. Furthermore, rise bacteria are resistant antibiotics presents an extra danger managing detrimental bacterium. Our review focuses on investigating presence analyzing various factors contribute extensive prevalence. We simplified complex mechanisms utilizes flourish human body, specific emphasis virulence antibiotic resistance. These variables pose challenges conventional In addition, we analyze both developing diagnostic methods, well current approaches implemented African nations. tackle distinct healthcare obstacles region put-up practical remedies. The main goal improve formulation more efficient methods for management HPI Africa.

Язык: Английский

---

Effect of lncRNA SNHG15 on LPS-induced vascular endothelial cell apoptosis, inflammatory factor expression and oxidative stress by targeting miR-362-3p

Cellular and Molecular Biology, Год журнала: 2022, Номер 67(6), С. 220 - 227

Опубликована: Фев. 27, 2022

This study aimed to investigate the effect of lncRNA SNHG15 targeting miR-362-3p on LPS-induced vascular endothelial cell apoptosis, inflammatory factor expression and oxidative stress. For this purpose, human umbilical vein cells (HUVECs) were treated with 100 ng/mL LPS for 24 hours establish a injury model. HUVECs divided into control, LPS, LPS+si-NC, LPS+si-SNHG15, LPS+miR-NC, LPS+miR-362-3p, LPS+si-SNHG15+anti-miR-NC LPS+si-SNHG15+anti-miR-362-3p groups. RT-qPCR was used determine miR-362-3pexpression. The inhibition rate measured by CCK-8 method; Cell apoptosis detected flow cytometry; kits employed detect intracellular SOD activity release LDH; ELISA method applied detcet levels TNF-α, IL-6 IL-10 in culture medium. Results showed that compared control group, rate, level group increased (P<0.05), LDH medium activity, level, reduced (P<0.05). LPS+si-SNHG15 LPS+miR-362-3p directly bound SNHG15. Compared In general, silencing inhibited stress response up-regulating expression.

Язык: Английский

---

Immunomodulatory effect of Ganoderma lucidum polysaccharide extract on peritoneal macrophage function of BALB/c mice

Cellular and Molecular Biology, Год журнала: 2022, Номер 68(4), С. 31 - 34

Опубликована: Апрель 30, 2022

Ganoderma lucidum has been suggested as a natural immunomodulator. It is not yet clear exactly which combination of this extract responsible for its immunomodulatory effects. Still, it appears that the 3-complement (CR3) receptor on surface immune cells acts beta-glucans (glucan-8), main component extract. Since glucose-6-phosphate dehydrogenase (G6PD) plays vital role in regulating macrophage functions, including nitric oxide production, we considered effect viability, G6PD enzyme activity, and (NO) production peritoneal BALB/c macrophages. First, macrophages mice were isolated treated with concentrations (0.001, 0.01, 0.1, 1, 10, 100 g/ml) polysaccharide (GL-PS). After 24 hours incubation by MTT test, evaluated viability macrophages, effective dose was determined to be 0.1g/ml. To determine specific activity glucose-6-phosphates, they incubated GI-PS at 0.1 mg/ml dose. Determination protein concentration obtained Bradford method cell supernatant Also, after 18 incubation, amount measured using Grace colorimetric method. According results, 0.1μg/ml had most significant (stimulation coefficient) compared other amounts (p <0.05). also found GL-PS increases NO lucidum, medicinal fungus, widely used East Asian countries, especially China, increase quality life longevity. study, concluded an activity. Therefore, fungus can strengthening agent phagocytic system against infectious agents pathogens such Leishmania parasite because essential defense them.

Язык: Английский

---

Usefulness of Molecular Methods for Helicobacter pylori Detection in Pediatric Patients and Their Correlation with Histopathological Sydney Classification

International Journal of Molecular Sciences, Год журнала: 2022, Номер 24(1), С. 179 - 179

Опубликована: Дек. 22, 2022

Helicobacter pylori infections, as one of the most prevalent among humans, are generally acquired during childhood, and main causes chronic gastritis peptic ulcer disease. A bacterial culture from a gastric biopsy is gold standard only method that has 100% specificity. However, its sensitivity varies, depending on experience laboratory staff, applied media, specimen transport conditions, site, quality sample. The same factors compromise all invasive methods culture-based H. infection diagnostic, well recent intake antibiotics, bismuth-containing compounds, proton pump inhibitors. Molecular have been used for clinical microbiology investigation since beginning 21st century. their usefulness infections diagnosis remains unclear, especially in pediatric patients. aim study was to assess incidence group 104 patients compare results PCR test with corresponding histopathological effects. Among samples collected children, 44 (42.3%) were positive PCR, while 43 (41.3%) 39 (37.5%) presented histologically-confirmed signs inflammation colonization, respectively. Moreover, mean grades parameters examination higher PCR-positive samples. compatibility both research confirmed, emphasizing molecular detecting Considering PCR-based gives reliable less time-consuming costly, it worth discussing this new at least patients, which diagnostics not sufficient or negative, observed macroscopically.

Язык: Английский

---

The effect of some trace elements on the expression of telomerase gene in lung cancer

Cellular and Molecular Biology, Год журнала: 2022, Номер 68(9), С. 86 - 90

Опубликована: Сен. 30, 2022

Lung cancer is a disorder that begins due to genetic and epigenetic changes. These changes cause the activation of oncogenes inactivation tumor suppressor genes. Various factors influence expression these In this research, we investigated relationship between number trace elements zinc copper ratio two in serum with telomerase enzyme gene lung cancer. For purpose, included 50 people study as case group 20 patients non-tumor diseases control group. The TRAP assay method measured activity biopsy samples tissue. Also, were by atomic absorption spectrometry. results showed mean concentration significantly higher than controls (120.8 ± 5.7 versus 107.2 6.5μg/dL)(P<0.05). However, there no significant difference groups (p > 0.05). patients' (1.6 0.4 1.1 0.2)(p < average level from 32.8 16.1 vs. zero percent) 0.01). There direct correlation (r = 0.36 p A positive was observed total increasing age 0.39 0.05), but amount negative -0/ 72 small cell carcinoma (123.7 2.8 μg/dL) compared non-small (117.6 4.8 high (P< Patients had (112 0.57%) carcinomas (6.4 2.5%)(P This situation regarding element evaluated non-significant (P>0.05). Based on obtained results, it can be assumed determining have biological role initiation progression tissue, which requires more studies.

Язык: Английский

---