Biology,
Год журнала:
2024,
Номер
13(10), С. 796 - 796
Опубликована: Окт. 5, 2024
Muscle
tissue
is
an
important
target
of
sex
steroids,
and
particularly,
testosterone
plays
essential
roles
in
muscle
cell
metabolism.
Wide
ranges
studies
have
reported
differences
basal
steroidogenesis,
recently
several
genes
been
identified
to
be
regulated
by
androgen
response
elements
that
show
innate
muscle.
However,
accounting
for
demonstrating
sexual
dimorphism
vitro
are
still
scarce
not
well
characterized.
Here,
we
demonstrated
the
ability
46XX
46XY
human
primary
skeletal
cells
differently
activate
steroidogenesis
vitro,
likely
related
sex-chromosome
onset,
induce
hormone
release
after
increasing
doses
exposure.
Cells
were
treated
with
at
concentrations
0.5,
2,
5,
10,
32,
100
nmol/L
24
h.
Variations
17β-HSD,
5α-R2,
CYP-19
expression,
DHT,
estradiol,
androstenedione
release,
as
IL6
IL8
analyzed,
respectively,
RT-PCR,
ELISA,
luminex-assay.
Following
treatments,
potentially
any
concentration
level,
increase
expression
was
observed
cells,
accompanied
elevated
levels
androstenedione,
IL6/IL8
release.
same
treatment,
exhibited
5α-R2
a
conversion
androgens
estrogens,
reduction
In
conclusion,
this
study
may
influence
homeostasis,
which
pivotal
Frontiers in Cell and Developmental Biology,
Год журнала:
2023,
Номер
11
Опубликована: Авг. 4, 2023
Neuromuscular
junctions
(NMJs)
are
a
special
type
of
chemical
synapse
that
transmits
electrical
stimuli
from
motor
neurons
(MNs)
to
their
innervating
skeletal
muscle
induce
response.
They
an
ideal
model
for
the
study
synapses,
given
manageable
size
and
easy
accessibility.
Alterations
in
morphology
or
function
lead
neuromuscular
disorders,
such
as
congenital
myasthenic
syndromes,
which
caused
by
mutations
proteins
located
NMJ.
In
this
review,
we
highlight
novel
potential
candidate
genes
may
cause
modify
NMJs-related
pathologies
humans
exploring
phenotypes
hundreds
mouse
models
available
literature.
We
also
underscore
fact
NMJs
differ
between
species,
muscles
even
sexes.
Hence
importance
choosing
good
organism
NMJ-related
diseases:
only
taking
into
account
specific
features
mammalian
NMJ,
experimental
results
would
be
efficiently
translated
clinic.
Journal of Oral Rehabilitation,
Год журнала:
2023,
Номер
50(7), С. 587 - 595
Опубликована: Март 23, 2023
Learning
and
memory
deficits
pathologic
changes
in
the
hippocampus
caused
by
toothlessness
soft
diet
feeding
are
related
to
reduced
masseter
muscle
(MM)
function.Myosin
heavy
chain
(MyHC)
isoform
expression
MM
also
under
different
chewing
conditions.
The
neurotransmitter
calcitonin
gene-related
peptide
(CGRP)
vascular
endothelial
growth
factor
A
(VEGF-A)
involved
formation.
However,
relationship
between
CGRP,
VEGF-A
MyHC
isoforms
senescence-accelerated
mouse
prone
8
(SAMP8)
strain,
a
model
of
learning
deficits,
remains
unclear.Changes
VEGF-A,
vasculogenesis
marker
mRNA
MMs
ageing
SAMP8
resistant
1
(SAMR1)
mice
was
investigated
through
quantitative
real-time
polymerase
reaction
(qRT-PCR)
situ
hybridization.qRT-PCR
revealed
obviously
high
CGRP
levels
(p
<
.001).
MyHC-IId/x
higher
24-week-old
than
SAMR1
.001)
but
lower
slow-MyHC
observed
on
fibres
not
hybridization.
Principal
component
analysis
(PCA)
strong
positive
contributions
SAMP8-MyHC-IId/x,
SAMP8-CGRP,
SAMR1-MyHC-emb,
SAMR1-CGRP,
SAMR1-VEGF-A,
SAMR1-CD31,
SAMP8-VEGF-A,
SAMP8-CD31
at
12
24
weeks.Calcitonin
is
key
for
patterns
mice.
Journal of Developmental Origins of Health and Disease,
Год журнала:
2024,
Номер
15
Опубликована: Янв. 1, 2024
Abstract
The
aim
of
this
study
was
to
analyse
the
expression
genes
related
regulation
energy
metabolism
in
skeletal
muscle
tissue
by
comparing
male
offspring
two
age
groups
[at
110
and
245
postnatal
days
(pnd)]
from
a
mother
with
obesity
induced
high-fat
diet
(-)-epicatechin
(Epi)
administration.
Four
six
different
litters
were
randomly
selected
for
control
[C
mothers
maternal
(MO)]
or
Epi
intervention
groups.
We
evaluated
effect
on
gastrocnemius
analysing
mRNA
protein
levels
Fndc5/irisin,
Pgc-1α,
Ucp3,
Sln.
significantly
increased
Pgc-1α
MO
group
at
pnd
(
p
<
0.036,
vs
.
MO+Epi),
while
pnd,
Fndc5/irisin
MO+Epi
versus
=
0.006).
No
differences
detected
Ucp3
Sln
(including
mRNA)
protein)
among
experimental
In
conclusion,
treatment
Pgc-α
245.
Furthermore,
it
is
suggested
that
flavonoid
model
its
impact
thermogenesis
are
regulated
pathway
than
Fndc5/irisin.
Biology,
Год журнала:
2024,
Номер
13(10), С. 796 - 796
Опубликована: Окт. 5, 2024
Muscle
tissue
is
an
important
target
of
sex
steroids,
and
particularly,
testosterone
plays
essential
roles
in
muscle
cell
metabolism.
Wide
ranges
studies
have
reported
differences
basal
steroidogenesis,
recently
several
genes
been
identified
to
be
regulated
by
androgen
response
elements
that
show
innate
muscle.
However,
accounting
for
demonstrating
sexual
dimorphism
vitro
are
still
scarce
not
well
characterized.
Here,
we
demonstrated
the
ability
46XX
46XY
human
primary
skeletal
cells
differently
activate
steroidogenesis
vitro,
likely
related
sex-chromosome
onset,
induce
hormone
release
after
increasing
doses
exposure.
Cells
were
treated
with
at
concentrations
0.5,
2,
5,
10,
32,
100
nmol/L
24
h.
Variations
17β-HSD,
5α-R2,
CYP-19
expression,
DHT,
estradiol,
androstenedione
release,
as
IL6
IL8
analyzed,
respectively,
RT-PCR,
ELISA,
luminex-assay.
Following
treatments,
potentially
any
concentration
level,
increase
expression
was
observed
cells,
accompanied
elevated
levels
androstenedione,
IL6/IL8
release.
same
treatment,
exhibited
5α-R2
a
conversion
androgens
estrogens,
reduction
In
conclusion,
this
study
may
influence
homeostasis,
which
pivotal
