The dependence of the ploidic status of embryos on the state of the spindle of oocyte division, assessed using polarization microscopy in IVF programs in women with ovarian endometriosis

Russian Bulletin of Obstetrician-Gynecologist, Год журнала: 2025, Номер 25(1), С. 44 - 44

Опубликована: Фев. 20, 2025

Objective. In infertile women with and without ovarian endometriosis, according to preimplantation genetic testing for aneuploidy, the ploidic status of embryos generated from mature oocytes (MII oocytes) a visually detectable division spindle should be clarified. Material methods. Using polarizing optical system OCTAX PolarAID, state was studied in 345 younger than 36 years healthy gonads (control group) 219 same age suffering endometriosis (main group). The influence presence/absence MII on frequency their correct fertilization, blastulation resulting evaluated. Preimplantation aneuploidy performed by Next Generation Sequencing (NGS). Results. cohort blastocysts that were obtained an unvisualized fission spindle, embryonic 2—3 times higher among normal state. This pattern reliable manifested itself extent endometriosis. Conclusion. When performing embryological stage IVF programs gonads, it is advisable clarify, using polarization microscopy, presence all isolated adult subsequently check pre-implantation testing, eggs undefined spindle.

Язык: Английский

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Beef cows with larger vulvar width have greater antral follicle count, viable oocytes, and higher circulating AMH

Animal Reproduction, Год журнала: 2025, Номер 22(1)

Опубликована: Янв. 1, 2025

Abstract Owing to the low heritability of reproductive traits, search for markers and their interrelationship that could indicate reproductively superior individuals is important in selection process bovine efficiency. This study aimed investigate possible interrelationships between antral follicle count (AFC), vulvar-width (VW), anti-Müllerian hormone (AMH) concentrations, fertility Bos Taurus Indicus females. Brahman (Bos Taurus-Indicus, n = 126) Simmental Angus Taurus-Taurus, 155) cows were classified as having large (≥86 mm) small (<86 VW. From each group, one blood sample per animal was collected determine AMH serum concentrations. The GLIMMIX procedure SAS® used whether vulva width (VW) classes, associated or not with breed, influence age at first calving (FCA), service interval (CFSI), (CI), number services pregnancy (SP), viable oocytes (VO). Antral (AFC) (36.10 ± 1.90 vs. 22.78 1.64, VW, respectively), (1.17 0.07 0.48 0.007 ng/mL), VO (18.86 1.76 10.15 1.49) greater (P < 0.05) VW than had AFC (36.30 1.34 22.09 1.67), (106.94 15.83 69.78 14.11 mm), (1.18 0.42 0.05 ng/mL) compared taurine cows. In conclusion, an efficient predictor concentrations both genetic groups, but under conditions this trial no link be detected these variables indices studied.

Язык: Английский

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Follicular development of fetal gonads under the skin of adult mice

Life Medicine, Год журнала: 2025, Номер 4(3)

Опубликована: Фев. 24, 2025

Adult ovarian tissues or biopsies isolated from patients prior to chemotherapy irradiation can reconstitute functions when transplanted either in the abdomen subcutaneously. Subcutaneously transplantation avoids invasive surgery and potential risks associated with internal procedures. We investigated whether functional ovaries could develop subcutaneously early E12.5 fetal gonads without entering meiosis mouse model. Unexpectedly, failed undergo folliculogenesis recipient mice. The experienced meiotic deficiency exhibited significant defects DNA repair recombination, increased apoptosis levels. Meiotic subcutaneous grafts were partly attributable variations temperature oxygen concentration. However, completion of prophase I was effectively achieved through vitro culture at 37°C. Subsequently, cultured gonads, following transplantation, became competent folliculogenesis, restoring endocrine functions. This finding may have implications for rejuvenating ovarioids gonad-like cells using pluripotent stem cell technologies, as well enhancing recovery health span.

Язык: Английский

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CCDC134 enhances ovarian reserve function and angiogenesis by directly interacting with INHA in a mouse model of premature ovarian insufficiency

APOPTOSIS, Год журнала: 2025, Номер unknown

Опубликована: Март 5, 2025

Язык: Английский

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Unveiling the Multifaceted Pharmacological Actions of Indole-3-Carbinol and Diindolylmethane: A Comprehensive Review

Plants, Год журнала: 2025, Номер 14(5), С. 827 - 827

Опубликована: Март 6, 2025

Cruciferae family vegetables are remarkably high in phytochemicals such as Indole-3-carbinol (I3C) and Diindolylmethane (DIM), which widely known nutritional supplements. I3C DIM have been studied extensively different types of cancers like breast, prostate, endometrial, colorectal, gallbladder, hepatic, cervical, well other tissues. In this review, we summarized the protective effects against cardiovascular, neurological, reproductive, metabolic, bone, respiratory, liver, immune diseases, infections, drug- radiation-induced toxicities. Experimental evidence suggests that offer protection due to their antioxidant, anti-inflammatory, antiapoptotic, immunomodulatory, xenobiotic properties. Apart from beneficial effects, present review also discusses possible toxicities reported various preclinical investigations. So far, most reports about diseases only studies; emphasizes dire need for large-scale clinical trials on these human diseases. Further, in-depth research is required improve bioavailability two achieve desirable effects. Overall, our may become potential drug candidates combating dreadful

Язык: Английский

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FERTILITY IN WOMEN WITH HEREDITARY BREAST AND OVARIAN CANCER

International Journal of Research -GRANTHAALAYAH, Год журнала: 2025, Номер 13(3)

Опубликована: Апрель 15, 2025

Among the causes of breast cancer and ovarian in women, mutations BRCA1/2 genes have been characterized detail. However, effect gene on female fertility remains controversial. The purpose this review is to assess relationship between BRCA1 BRCA2 mutation status its various manifestations. A number scientists consider as a negative factor that reduces reproductive abilities women. study oocytes showed possibility spontaneous inactivation X chromosome, which can lead infertility offspring, well significantly reduced reserve low oocyte yield. There are studies devoted assessment expression young old oocytes, decrease protein by DNA repair parallel with age rodents, cattle humans. series confirm significant follicles women carriers BRCA1/2. an opinion do not affect these mutations. These based epidemiological demographic data. attribute presence BRCA1\2 factors increase fertility. They base their point view longer telomeres cells carriers. In carriers, average telomere length than relatives who (P = 0.0018), especially families 0.0016). authors rely such well-known phenomenon antagonistic pleiotropy. mechanisms linking remain subject debate fully studied. Animal model may reveal new

Язык: Английский

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From TCM “Shen-nourishing” and “Yang-strengthening” theory to Blood-Testis Barrier Reorganization,GuiLuBuShen Attenuates Age-Related Male Reproductive Dysfunction

Journal of Ethnopharmacology, Год журнала: 2025, Номер unknown, С. 119899 - 119899

Опубликована: Май 1, 2025

Язык: Английский

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A case-control study on the link between trace element exposure in follicular fluid and premature ovarian insufficiency

Reproductive Toxicology, Год журнала: 2025, Номер unknown, С. 108947 - 108947

Опубликована: Май 1, 2025

Язык: Английский

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Dysregulation of AR and ERα caused ovarian alterations in gerbils prenatally exposed to 17α-ethinylestradiol and pequi oil

Histochemistry and Cell Biology, Год журнала: 2025, Номер 163(1)

Опубликована: Май 22, 2025

Язык: Английский

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Pleiotropic Signaling by Reactive Oxygen Species Concerted with Dietary Phytochemicals and Microbial-Derived Metabolites as Potent Therapeutic Regulators of the Tumor Microenvironment

Antioxidants, Год журнала: 2023, Номер 12(5), С. 1056 - 1056

Опубликована: Май 6, 2023

The excessive generation of reactive oxygen species (ROS) plays a pivotal role in the pathogenesis diseases. ROS are central to cellular redox regulation and act as second messengers activate redox-sensitive signals. Recent studies have revealed that certain sources can be beneficial or harmful human health. Considering essential pleiotropic roles basic physiological functions, future therapeutics should designed modulate state. Dietary phytochemicals, microbiota, metabolites derived from them expected developed drugs prevent treat disorders tumor microenvironment.

Язык: Английский

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