Active avoidance recruits the anterior cingulate cortex regardless of social context in male and female rats. DOI Creative Commons

Shannon Ruble,

Cassandra Kramer,

Lexe West

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июнь 7, 2024

Abstract Actively avoiding danger is necessary for survival. Most research has focused on the behavioral and neurobiological processes when individuals avoid alone, under solitary conditions. Therefore, little known about how social context affects active avoidance. Using a modified version of platform-mediated avoidance task in rats, we investigated whether presence partner attenuates conditioned freezing enhances learning compared to learned Rats spent similar percentage time during tone both conditions; however, rats trained conditions exhibited greater as well lower rates darting food seeking rats. Under conditions, observed higher levels females males, which was not present To gain mechanistic insight, optogenetically inactivated glutamatergic projection neurons anterior cingulate cortex (ACC) following training. Photoinactivation ACC reduced expression absence partner. photoinactivation delayed males but blocked females. Our findings suggest that mediated by ACC, regardless context, may be dysfunctional those suffering from trauma-related disorders. Furthermore, sex differences prefrontal circuits mediating warrant further investigation, given experience risk developing anxiety

Язык: Английский

Transformations in prefrontal ensemble activity underlying rapid threat avoidance learning DOI Creative Commons
Christopher Gabriel, Tanya Gupta,

Asai Sánchez-Fuentes

и другие.

Current Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

2
D-LMBmap: a fully automated deep-learning pipeline for whole-brain profiling of neural circuitry DOI Creative Commons
Zhongyu Li, Zengyi Shang, Jingyi Liu

и другие.

Nature Methods, Год журнала: 2023, Номер 20(10), С. 1593 - 1604

Опубликована: Сен. 28, 2023

Recent proliferation and integration of tissue-clearing methods light-sheet fluorescence microscopy has created new opportunities to achieve mesoscale three-dimensional whole-brain connectivity mapping with exceptionally high throughput. With the rapid generation large, high-quality imaging datasets, downstream analysis is becoming major technical bottleneck for connectomics. Current computational solutions are labor intensive limited applications because exhaustive manual annotation heavily customized training. Meanwhile, data always requires combining multiple packages secondary development by users. To address these challenges, we developed D-LMBmap, an end-to-end package providing integrated workflow containing three modules based on deep-learning algorithms mapping: axon segmentation, brain region segmentation registration. D-LMBmap does not require achieves quantitative projectome in a single superior accuracy cell types all modalities tested.

Язык: Английский

Процитировано

19
Behavioral outputs and overlapping circuits between conditional fear and active avoidance DOI
María M. Diehl, Justin M. Moscarello, Sydney Trask

и другие.

Neurobiology of Learning and Memory, Год журнала: 2024, Номер 213, С. 107943 - 107943

Опубликована: Май 29, 2024

Язык: Английский

Процитировано

5
A history of avoidance does not impact extinction learning in male rats DOI Creative Commons
Alba López-Moraga, Laura Luyten, Tom Beckers

и другие.

npj Science of Learning, Год журнала: 2024, Номер 9(1)

Опубликована: Фев. 24, 2024

Pervasive avoidance is one of the central symptoms all anxiety-related disorders. In treatment, behaviors are typically discouraged because they assumed to maintain anxiety. Yet, it not clear if engaging in always detrimental. this study, we used a platform-mediated task investigate influence history on extinction learning male rats. Our results show that having opportunity avoid during fear acquisition training does significantly auditory-cued rats subjected procedure, which constitutes realistic approach/avoidance conflict. This holds true irrespective whether or was possible phase. suggests imposing cost behavior prevents adverse effects has been claimed have extinction. However, appear positive nor retention either.

Язык: Английский

Процитировано

4
Microglia regulate nucleus accumbens synaptic development and circuit function underlying threat avoidance behaviors DOI Creative Commons
Michael W. Gongwer, Fanny Etienne,

Eric N. Moca

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 16, 2025

Abstract While CNS microglia have well-established roles in synapse pruning during neurodevelopment, only a few studies identified for formation. These focused on the cortex and primary sensory circuits restricted developmental time periods, leaving substantial gaps our understanding of early functions microglia. Here we investigated how absence impacts synaptic development nucleus accumbens (NAc), region critical emotional regulation motivated behaviors where dysfunction is implicated psychiatric disorders that arise life. Using genetically modified mouse lacks ( Csf1r ΔFIRE/ΔFIRE ), found blunted excitatory formation NAc. This effect was most prominent second third postnatal weeks, when previously to be overproduced, accompanied by an increase presynaptic release probability alterations postsynaptic kinetics. Tissue-level NAc proteomics confirmed microglial impacted numerous proteins involved structure, trans-synaptic signaling, pre-synaptic function. However, did not perturb levels astrocyte-derived cues adhesive promote synaptogenesis, suggesting reduced number may caused microglial-derived synaptogenic cue. Although observed electrophysiological changes were largely normalized adulthood, lasting effects threat avoidance behavior, these behavioral directly associated with neuronal activity. Together, results indicate role regulating landscape developing establishing functional underlying adult repertoires.

Язык: Английский

Процитировано

0
A cell type-specific mechanism driving the rapid antidepressant effects of transcranial magnetic stimulation DOI Creative Commons
Michael W. Gongwer,

Alex Qi,

Alexander S. Enos

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 30, 2025

Abstract Repetitive transcranial magnetic stimulation (rTMS) is an emerging treatment for brain disorders, but its therapeutic mechanism unknown. We developed a novel mouse model of rTMS with superior clinical face validity and investigated the neural by which accelerated intermittent theta burst (aiTBS) – first rapid-acting antidepressant protocol reversed chronic stress-induced behavioral deficits. Using fiber photometry, we showed that aiTBS drives distinct patterns activity in intratelencephalic (IT) pyramidal tract (PT) projecting neurons dorsomedial prefrontal cortex (dmPFC). However, only IT exhibited persistently increased during both subsequent depression-related behaviors. Similarly, stress-related loss dendritic spines on IT, not PT neurons, further demonstrating cell type-specific effects stimulation. Finally, chemogenetic inhibition dmPFC blocked antidepressant-like aiTBS. Thus, demonstrate linking rapid aiTBS-driven to circuit plasticity.

Язык: Английский

Процитировано

0
Distinct prelimbic cortex neuronal responses to emotional states of others drive emotion recognition in adult mice DOI
Renad Jabarin, Alok Nath Mohapatra,

Natali Ray

и другие.

Current Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Basal Forebrain Projections to the Retrosplenial and Cingulate Cortex in Rats DOI Creative Commons
Hideki Kondo, László Záborszky

The Journal of Comparative Neurology, Год журнала: 2025, Номер 533(2)

Опубликована: Фев. 1, 2025

The basal forebrain (BF) plays a crucial role in modulating cortical activation through its widespread projections across the mantle. Previous anatomical studies have demonstrated that each region receives specific projection from BF. In this study, we examined BF cholinergic and non-cholinergic to retrosplenial cortex (RSC) anterior cingulate (ACC) using two retrograde tracers, Fast Blue (FB) Fluoro-Gold (FG), combination with choline acetyltransferase (ChAT) immunostaining rats. RSC ACC receive mainly medial part of horizontal limb diagonal band (HDB) vertical (VDB). main difference RSC, ACC, prelimbic (PL) is PL rostral half globus pallidus (GP), whereas stronger VDB septum (MS). As injection site shifts caudal strong GP weak MS/VDB neurons are reversed. Cholinergic make up similar proportion total both (37%) (33%) injections. Double tracer injections revealed small number double-labeled HDB. These findings indicate spatially overlapping differential BF, varying between subregions different rostrocaudal regions.

Язык: Английский

Процитировано

0
Developmentally distinct architectures in top–down pathways controlling threat avoidance DOI

Cassandra B. Klune,

Caitlin M. Goodpaster, Michael W. Gongwer

и другие.

Nature Neuroscience, Год журнала: 2025, Номер unknown

Опубликована: Фев. 19, 2025

Язык: Английский

Процитировано

0
Developmental changes in brain-wide fear memory networks DOI Creative Commons
Benita Jin, Michael W. Gongwer, Laura A. DeNardo

и другие.

Neurobiology of Learning and Memory, Год журнала: 2025, Номер unknown, С. 108037 - 108037

Опубликована: Март 1, 2025

Memory retrieval involves coordinated activity across multiple brain regions. Yet how the organization of memory networks evolves throughout development remains poorly understood. In this study, we compared whole-brain functional that are active during contextual fear recall in infant, juvenile, and adult mice. Our analyses revealed long-term change significantly postnatal development. Infant dense heterogeneous, whereas sparse have a small-world topology. While hippocampal subregions were highly connected nodes at all ages, cortex gained many connections Different matured different rates, but their developmental timing fell into three major categories: stepwise between two linear or inverted-U, with elevated connectivity juveniles. work highlights subset regions likely maintain important roles encoding, undergoes significant reorganization Together, these results provide blueprint for studying correlated cellular key areas distinctly regulates storage

Язык: Английский

Процитировано

0