Regional and cell-type-specific afferent and efferent projections of the mouse claustrum

Cell Reports, Год журнала: 2023, Номер 42(2), С. 112118 - 112118

Опубликована: Фев. 1, 2023

The claustrum (CLA) is a conspicuous subcortical structure interconnected with cortical and regions. Its regional anatomy cell-type-specific connections in the mouse remain not fully determined. Using multimodal reference datasets, we confirmed delineation of CLA as single group neurons embedded agranular insular cortex. We quantitatively investigated brain-wide inputs outputs using bulk anterograde retrograde viral tracing data neuron data. found that prefrontal module has more cell types projecting to than other modules, layer 5 IT predominating. nine morphological principal topographically innervate functionally linked targets, preferentially midline areas, secondary motor area, entorhinal area. Together, this study provides detailed wiring diagram CLA, laying foundation for studying its functions at cellular level.

Язык: Английский

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Neural activity in the mouse claustrum in a cross-modal sensory selection task

Neuron, Год журнала: 2021, Номер 110(3), С. 486 - 501.e7

Опубликована: Дек. 3, 2021

Язык: Английский

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Influence of claustrum on cortex varies by area, layer, and cell type

Neuron, Год журнала: 2022, Номер 111(2), С. 275 - 290.e5

Опубликована: Ноя. 10, 2022

Язык: Английский

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Single-cell spatial transcriptome atlas and whole-brain connectivity of the macaque claustrum

Cell, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Claustrum orchestrates brain functions via its connections with numerous regions, but molecular and cellular organization remains unresolved. Single-nucleus RNA sequencing of 227,750 macaque claustral cells identified 48 transcriptome-defined cell types, most glutamatergic neurons similar to deep-layer insular neurons. Comparison macaque, marmoset, mouse transcriptomes revealed macaque-specific types. Retrograde tracer injections at 67 cortical 7 subcortical regions defined four distinct distribution zones retrogradely labeled Joint analysis whole-brain connectivity single-cell spatial transcriptome showed that these containing compositions (but not GABAergic) types preferentially connected specific a strong ipsilateral bias. Several in ventral vs. dorsal selectively co-projected two functionally related areas-entorhinal cortex hippocampus motor putamen, respectively. These data provide the basis for elucidating neuronal underlying diverse functions.

Язык: Английский

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Claustral Neurons Projecting to Frontal Cortex Mediate Contextual Association of Reward

Current Biology, Год журнала: 2020, Номер 30(18), С. 3522 - 3532.e6

Опубликована: Июль 23, 2020

The claustrum is a small nucleus, exhibiting vast reciprocal connectivity with cortical, subcortical, and midbrain regions. Recent studies, including ours, implicate the in salience detection attention. In current study, we develop an iterative functional investigation of claustrum, guided by quantitative spatial transcriptional analysis. Using this approach, identify circuit involving dopamine-receptor expressing claustral neurons projecting to frontal cortex necessary for context association reward. We describe recruitment cocaine their role drug sensitization. order characterize within which these are embedded, apply chemo- opto-genetic manipulation increasingly specified subpopulations. This strategy resolves defined network dopamine D1 receptors acquisition conditioned-place preference real-time optogenetic preference. sum, our results suggest claustrum-to-frontal attribution incentive salience, allocating attention reward-related contextual cues.

Язык: Английский

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Repetitive mild traumatic brain injury in mice triggers a slowly developing cascade of long-term and persistent behavioral deficits and pathological changes

Acta Neuropathologica Communications, Год журнала: 2021, Номер 9(1)

Опубликована: Апрель 6, 2021

Abstract We have previously reported long-term changes in the brains of non-concussed varsity rugby players using magnetic resonance spectroscopy (MRS), diffusion tensor imaging (DTI) and functional (fMRI). Others cognitive deficits contact sport athletes that not met diagnostic criteria for concussion. These results suggest repetitive mild traumatic brain injuries (rmTBIs) are severe enough to meet threshold concussion, produce consequences. sought characterize neuroimaging, cognitive, pathological metabolomic a mouse model rmTBI. Using closed-skull mTBI when scaled human leads rotational linear accelerations far below what has been sports concussion athletes, we found 5 daily mTBIs triggered two temporally distinct types changes. First, during first days weeks after injury, rmTBI produced diffuse axonal transient inflammatory response resolved with time. Second, led were evident months injury including: altered levels synaptic proteins, behavioural attention spatial memory, accumulations pathologically phosphorylated tau, blood profiles white matter ultrastructural abnormalities. indicate exceedingly rmTBI, mice, triggers processes consequences observable initial injury.

Язык: Английский

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Pain-induced adaptations in the claustro-cingulate pathway

Cell Reports, Год журнала: 2023, Номер 42(5), С. 112506 - 112506

Опубликована: Май 1, 2023

Persistent pain is a prevalent medical concern correlating with hyperexcitable anterior cingulate cortex (ACC). Its activity modulated by inputs from several brain regions, but the maladjustments that these afferent circuits undergo during transition acute to chronic still require clarification. We focus on ACC-projecting claustrum (CLAACC) neurons and their responses sensory aversive stimuli in mouse model of inflammatory pain. Using chemogenetics, vivo calcium imaging, ex electrophysiological approaches, we reveal suppression CLAACC acutely attenuates allodynia preferentially transmits information ACC. With prolonged pain, claustro-cingulate functional impairment develops, which mediated weakened excitatory drive onto ACC pyramidal neurons, resulting diminished claustral influence These findings support an instrumental role processing nociceptive its susceptibility persistent states.

Язык: Английский

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Claustrum neurons projecting to the anterior cingulate restrict engagement during sleep and behavior

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Июнь 26, 2024

Abstract The claustrum has been linked to attention and sleep. We hypothesized that this reflects a shared function, determining responsiveness stimuli, which spans the axis of engagement. To test hypothesis, we recorded population dynamics from male mice during both sleep an attentional task (‘ENGAGE’). Heightened activity in neurons projecting anterior cingulate cortex (ACCp) corresponded reduced sensory Similarly, ENGAGE task, heightened ACCp correlated with disengagement behavioral lapses, while low hyper-engagement impulsive errors. Chemogenetic elevation awakenings errors task. Furthermore, employing exploration strategy showed stronger correlation between performance compared exploitation complexity. Our results implicate restricting engagement goal-directed behavior.

Язык: Английский

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Topographic gradients define the projection patterns of the claustrum core and shell in mice

The Journal of Comparative Neurology, Год журнала: 2020, Номер 529(7), С. 1607 - 1627

Опубликована: Сен. 25, 2020

The claustrum is densely connected to the cortex and participates in brain functions such as attention sleep. Although some studies have reported widely divergent organization of projections, others describe parallel claustrocortical connections different cortical regions. Therefore, details underlying how neurons broadcast information networks remain incompletely understood. Using multicolor retrograde tracing we determined density, topography, co-projection pattern 14 pathways, mice. We spatially registered these pathways a common coordinate space found that system topographically organized series overlapping spatial modules, continuously distributed across dorsoventral axis. core projects predominantly frontal-midline regions, whereas dorsal ventral shell project motor temporal lobe, respectively. Anatomically regions receive input from subset shared by neighboring separated are innervated modules. each output module exhibits unique position within overlaps substantially with other modules projecting functionally related Claustrum inhibitory cells containing parvalbumin, somatostatin, neuropeptide Y also show topographical distributions, suggesting controlled distinct circuit motifs. topographic excitatory cell types may enable outputs independently networks.

Язык: Английский

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The claustrum-medial prefrontal cortex network controls attentional set-shifting

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2020, Номер unknown

Опубликована: Окт. 15, 2020

SUMMARY In various mental disorders, dysfunction of the prefrontal cortex contributes to cognitive deficits. Here we studied how claustrum (CLA), a nucleus sharing reciprocal connections with cortex, may participate in these impairments. We show that specific ensembles CLA and medial (mPFC) neurons are activated during task requiring control such as attentional set-shifting, i.e. ability shift attention towards newly relevant stimulus-reward associations while disengaging from irrelevant ones. exert direct excitatory input on mPFC pyramidal cells, chemogenetic inhibition suppresses formation assemblies set-shifting. Furthermore, impairing recruitment through opto/chemogenetic manipulations prevents conclusion, propose controls reorganization enable emphasizing potential role CLA-mPFC network dysfunctions.

Язык: Английский

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