Bidirectional plasticity of GABAergic tonic inhibition in hippocampal somatostatin- and parvalbumin-containing interneurons

Frontiers in Cellular Neuroscience, Год журнала: 2023, Номер 17

Опубликована: Июнь 28, 2023

GABA A receptors present in extrasynaptic areas mediate tonic inhibition hippocampal neurons regulating the performance of neural networks. In this study, we investigated effect NMDA-induced plasticity on somatostatin- and parvalbumin-containing interneurons. Using pharmacological methods transgenic mice (SST-Cre/PV-Cre x Ai14), induced GABAergic transmission interneurons by a brief (3 min) application NMDA. whole-cell patch-clamp configuration, measured currents enhanced specific agonists (etomidate or gaboxadol). Furthermore, both control NMDA-treated groups, examined to what extent these changes depend regulation distinct subtypes receptors. Tonic conductance somatostatin-containing (SST+) is after NMDA application, observed associated with an increased content α5-containing Rs. Both fast-spiking non–fast-spiking parvalbumin-positive (PV+) cells showed reduction induction. This was accompanied PV+ interneuron types strongly reduced proportion δ-subunit-containing Rs relatively small increase mediated show cell type-dependent opposite sign inhibition. The underlying mechanisms cell-specific balance plastic contents α5 δ subunit-containing

Язык: Английский

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Mechanism of Antidepressant Action of (2R,6R)-6-Hydroxynorketamine (HNK) and Its Compounds: Insights from Proteomic Analysis

Molecular Neurobiology, Год журнала: 2023, Номер unknown

Опубликована: Авг. 26, 2023

Язык: Английский

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Extracellular matrix integrity regulates GABAergic plasticity in the hippocampus

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 30, 2024

Abstract The brain’s extracellular matrix (ECM) is crucial for neural circuit functionality, synaptic plasticity, and learning. While the role of ECM in excitatory synapses has been extensively studied, its influence on inhibitory synapses, particularly GABAergic long-term remains poorly understood. This study aims to elucidate effects components transmission plasticity hippocampal CA1 region. We focus roles chondroitin sulfate proteoglycans (CSPGs) hyaluronic acid modulating postsynaptic currents (IPSCs) at two distinct formed by somatostatin (SST)-positive parvalbumin (PV)-positive interneurons onto pyramidal cells (PCs). Using optogenetic stimulation brain slices, we observed that acute degradation constituents hyaluronidase or chondroitinase-ABC did not affect basal transmission. However, short-term burst-induced depression, was enhanced PV→PC following enzymatic treatments. Long-term experiments demonstrated CSPGs are essential NMDA-induced iLTP SST→PC whereas digestion impaired synapses. indicates a synapse-specific regulating plasticity. Additionally, report presence cryptic induced prolonged NMDA application, which became evident after CSPG absent under control conditions. Our results underscore differential impact highlighting interplay between specific offers new perspectives studies learning critical period timing.

Язык: Английский

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Cholecystokinin-expressing GABA neurons elicit long-term potentiation in the cortical inhibitory synapses and attenuate sound-shock associative memory

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 8, 2024

Abstract Neuronal interactions between inhibitory and excitatory neurons play a pivotal role in regulating the balance of excitation inhibition central nervous system (CNS). Consequently, efficacy inhibitory/excitatory synapses profoundly affects neural network processing overall neuronal functions. Here, we describe novel form long-term potentiation (LTP) induced at cortical its behavioral consequences. We show that high-frequency laser stimulation (HFLS) GABAergic elicit LTP (i-LTP) pyramidal auditory cortex (AC). The selective activation cholecystokinin-expressing GABA (GABA CCK ) is essential for formation HFLS-induced i-LTP, rather than classical parvalbumin (PV) somatostatin (SST) neurons. Intriguingly, i-LTP can be evoked AC by adding exogenous neuropeptide when PV SST are selectively activated PV-Cre SST-Cre mice, respectively. Additionally, discovered low-frequency (LFLS) paired with HFLS potentiates effect interneurons on neurons, thereby generating heterosynaptic AC. Notably, light CCK-Cre mice significantly attenuates sound-shock associative memory, while does not affect this memory mice. In conclusion, these results demonstrate critical mechanism excitation-inhibition modulating learning circuits. This might serve as potential target treatment neurological disorders, including epilepsy Alzheimer’s disease.

Язык: Английский

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Extracellular matrix integrity regulates GABAergic plasticity in the hippocampus

Matrix Biology, Год журнала: 2024, Номер 134, С. 184 - 196

Опубликована: Ноя. 2, 2024

Язык: Английский

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