A marmoset brain cell census reveals regional specialization of cellular identities DOI Creative Commons
Fenna M. Krienen, Kirsten M. Levandowski, Heather Zaniewski

и другие.

Science Advances, Год журнала: 2023, Номер 9(41)

Опубликована: Окт. 12, 2023

The mammalian brain is composed of many structures, each with its own ontogenetic and developmental history. We used single-nucleus RNA sequencing to sample over 2.4 million cells across 18 locations in the common marmoset, a New World monkey primed for genetic engineering, examined gene expression patterns cell types within structures. adult transcriptomic identity most neuronal shaped more by origin than neurotransmitter signaling repertoire. Quantitative mapping GABAergic single-molecule FISH (smFISH) reveals that interneurons striatum neocortex follow distinct spatial principles, lateral prefrontal other higher-order cortical association areas are distinguished high proportions

Язык: Английский

Integrated Morphoelectric and Transcriptomic Classification of Cortical GABAergic Cells DOI Creative Commons
Nathan W. Gouwens, Staci A. Sorensen, Fahimeh Baftizadeh

и другие.

Cell, Год журнала: 2020, Номер 183(4), С. 935 - 953.e19

Опубликована: Ноя. 1, 2020

Язык: Английский

Процитировано

451

Innovations present in the primate interneuron repertoire DOI
Fenna M. Krienen, Melissa Goldman, Qiangge Zhang

и другие.

Nature, Год журнала: 2020, Номер 586(7828), С. 262 - 269

Опубликована: Сен. 30, 2020

Язык: Английский

Процитировано

313

Phenotypic variation of transcriptomic cell types in mouse motor cortex DOI Creative Commons
Federico Scala, Dmitry Kobak, Matteo Bernabucci

и другие.

Nature, Год журнала: 2020, Номер 598(7879), С. 144 - 150

Опубликована: Ноя. 12, 2020

Abstract Cortical neurons exhibit extreme diversity in gene expression as well morphological and electrophysiological properties 1,2 . Most existing neural taxonomies are based on either transcriptomic 3,4 or morpho-electric 5,6 criteria, it has been technically challenging to study both aspects of neuronal the same set cells 7 Here we used Patch-seq 8 combine patch-clamp recording, biocytin staining, single-cell RNA sequencing more than 1,300 adult mouse primary motor cortex, providing a annotation almost all transcriptomically defined cell types. We found that, although broad families types (those expressing Vip , Pvalb Sst so on) had distinct essentially non-overlapping phenotypes, individual within family were not separated space. Instead, there was continuum variability morphology electrophysiology, with neighbouring showing similar features, often without clear boundaries between them. Our results suggest that neocortex do always form discrete entities. hierarchy consists branches at level families, but can continuous correlated morpho-electrical landscapes families.

Язык: Английский

Процитировано

301

Circuit organization of the rodent medial prefrontal cortex DOI
Paul G. Anastasiades, Adam G. Carter

Trends in Neurosciences, Год журнала: 2021, Номер 44(7), С. 550 - 563

Опубликована: Май 7, 2021

Язык: Английский

Процитировано

208

Untangling the cortico-thalamo-cortical loop: cellular pieces of a knotty circuit puzzle DOI
Gordon M. Shepherd, Naoki Yamawaki

Nature reviews. Neuroscience, Год журнала: 2021, Номер 22(7), С. 389 - 406

Опубликована: Май 6, 2021

Язык: Английский

Процитировано

191

All-Optical Electrophysiology Reveals the Role of Lateral Inhibition in Sensory Processing in Cortical Layer 1 DOI Creative Commons
Linlin Z. Fan, Simon Kheifets, Urs L. Böhm

и другие.

Cell, Год журнала: 2020, Номер 180(3), С. 521 - 535.e18

Опубликована: Янв. 23, 2020

Язык: Английский

Процитировано

148

Comprehensive Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional Payloads to Cells of Behaving Mammals DOI Creative Commons
Lief E. Fenno, Charu Ramakrishnan, Yoon Seok Kim

и другие.

Neuron, Год журнала: 2020, Номер 107(5), С. 836 - 853.e11

Опубликована: Июнь 22, 2020

Язык: Английский

Процитировано

147

Human prefrontal cortex gene regulatory dynamics from gestation to adulthood at single-cell resolution DOI Creative Commons
Charles A. Herring, Rebecca K. Simmons, Saskia Freytag

и другие.

Cell, Год журнала: 2022, Номер 185(23), С. 4428 - 4447.e28

Опубликована: Окт. 31, 2022

Human brain development is underpinned by cellular and molecular reconfigurations continuing into the third decade of life. To reveal cell dynamics orchestrating neural maturation, we profiled human prefrontal cortex gene expression chromatin accessibility at single-cell resolution from gestation to adulthood. Integrative analyses define dynamic trajectories each type, revealing major reconfiguration prenatal-to-postnatal transition in all types followed continuous adulthood identifying regulatory networks guiding developmental programs, states, functions. We uncover links between milestones, characterize diverse timing when cells acquire adult-like identify convergence distinct origins. further their regulators implicated neurological disorders. Finally, using this reference, benchmark identities maturation states organoid models. Together, captures landscape cortical development.

Язык: Английский

Процитировано

131

Neocortical Layer 1: An Elegant Solution to Top-Down and Bottom-Up Integration DOI Open Access
Benjamin Schuman,

Shlomo S. Dellal,

Alvar Prönneke

и другие.

Annual Review of Neuroscience, Год журнала: 2021, Номер 44(1), С. 221 - 252

Опубликована: Март 17, 2021

Many of our daily activities, such as riding a bike to work or reading book in noisy cafe, and highly skilled professional playing tennis match violin concerto, depend upon the ability brain quickly make moment-to-moment adjustments behavior response results actions. Particularly, they neocortex integrate information provided by sensory organs (bottom-up information) with internally generated signals expectations attentional (top-down information). This integration occurs pyramidal cells (PCs) their long apical dendrite, which branches extensively into dendritic tuft layer 1 (L1). The outermost neocortex, L1 is conserved across cortical areas species. Importantly, predominant input for top-down information, relayed rich, dense mesh long-range projections that provide PCs. Here, we discuss recent progress understanding composition review evidence processing contributes functions perception, cross-modal integration, controlling states consciousness, attention, learning.

Язык: Английский

Процитировано

128

A transcriptomic axis predicts state modulation of cortical interneurons DOI Creative Commons
Stéphane Bugeon, Joshua Duffield, Mario Dipoppa

и другие.

Nature, Год журнала: 2022, Номер 607(7918), С. 330 - 338

Опубликована: Июль 6, 2022

Abstract Transcriptomics has revealed that cortical inhibitory neurons exhibit a great diversity of fine molecular subtypes 1–6 , but it is not known whether these have correspondingly diverse patterns activity in the living brain. Here we show primary visual cortex (V1) correlates with brain state, which are organized by single factor: position along main axis transcriptomic variation. We combined vivo two-photon calcium imaging mouse V1 method to identify mRNA for 72 selected genes ex slices. classified imaged layers 1–3 into three-level hierarchy 5 subclasses, 11 types and 35 using previously defined clusters 3 . Responses stimuli differed significantly only between cells Sncg subclass uniformly suppressed, other subclasses predominantly excited. Modulation state at all hierarchical levels could be largely predicted from first principal component, also correlations simultaneously recorded cells. Inhibitory fired more resting, oscillatory states had smaller fraction their axonal projections layer 1, narrower spikes, lower input resistance weaker adaptation as determined vitro 7 expressed cholinergic receptors. Subtypes during arousal opposite properties. Thus, simple principle may explain how shape state-dependent processing.

Язык: Английский

Процитировано

125