Integrated Morphoelectric and Transcriptomic Classification of Cortical GABAergic Cells

Cell, Год журнала: 2020, Номер 183(4), С. 935 - 953.e19

Опубликована: Ноя. 1, 2020

Язык: Английский

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Innovations present in the primate interneuron repertoire

Nature, Год журнала: 2020, Номер 586(7828), С. 262 - 269

Опубликована: Сен. 30, 2020

Язык: Английский

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Phenotypic variation of transcriptomic cell types in mouse motor cortex

Nature, Год журнала: 2020, Номер 598(7879), С. 144 - 150

Опубликована: Ноя. 12, 2020

Abstract Cortical neurons exhibit extreme diversity in gene expression as well morphological and electrophysiological properties 1,2 . Most existing neural taxonomies are based on either transcriptomic 3,4 or morpho-electric 5,6 criteria, it has been technically challenging to study both aspects of neuronal the same set cells 7 Here we used Patch-seq 8 combine patch-clamp recording, biocytin staining, single-cell RNA sequencing more than 1,300 adult mouse primary motor cortex, providing a annotation almost all transcriptomically defined cell types. We found that, although broad families types (those expressing Vip , Pvalb Sst so on) had distinct essentially non-overlapping phenotypes, individual within family were not separated space. Instead, there was continuum variability morphology electrophysiology, with neighbouring showing similar features, often without clear boundaries between them. Our results suggest that neocortex do always form discrete entities. hierarchy consists branches at level families, but can continuous correlated morpho-electrical landscapes families.

Язык: Английский

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Circuit organization of the rodent medial prefrontal cortex

Trends in Neurosciences, Год журнала: 2021, Номер 44(7), С. 550 - 563

Опубликована: Май 7, 2021

Язык: Английский

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Untangling the cortico-thalamo-cortical loop: cellular pieces of a knotty circuit puzzle

Nature reviews. Neuroscience, Год журнала: 2021, Номер 22(7), С. 389 - 406

Опубликована: Май 6, 2021

Язык: Английский

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All-Optical Electrophysiology Reveals the Role of Lateral Inhibition in Sensory Processing in Cortical Layer 1

Cell, Год журнала: 2020, Номер 180(3), С. 521 - 535.e18

Опубликована: Янв. 23, 2020

Язык: Английский

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Comprehensive Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional Payloads to Cells of Behaving Mammals

Neuron, Год журнала: 2020, Номер 107(5), С. 836 - 853.e11

Опубликована: Июнь 22, 2020

Язык: Английский

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Human prefrontal cortex gene regulatory dynamics from gestation to adulthood at single-cell resolution

Cell, Год журнала: 2022, Номер 185(23), С. 4428 - 4447.e28

Опубликована: Окт. 31, 2022

Human brain development is underpinned by cellular and molecular reconfigurations continuing into the third decade of life. To reveal cell dynamics orchestrating neural maturation, we profiled human prefrontal cortex gene expression chromatin accessibility at single-cell resolution from gestation to adulthood. Integrative analyses define dynamic trajectories each type, revealing major reconfiguration prenatal-to-postnatal transition in all types followed continuous adulthood identifying regulatory networks guiding developmental programs, states, functions. We uncover links between milestones, characterize diverse timing when cells acquire adult-like identify convergence distinct origins. further their regulators implicated neurological disorders. Finally, using this reference, benchmark identities maturation states organoid models. Together, captures landscape cortical development.

Язык: Английский

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Neocortical Layer 1: An Elegant Solution to Top-Down and Bottom-Up Integration

Annual Review of Neuroscience, Год журнала: 2021, Номер 44(1), С. 221 - 252

Опубликована: Март 17, 2021

Many of our daily activities, such as riding a bike to work or reading book in noisy cafe, and highly skilled professional playing tennis match violin concerto, depend upon the ability brain quickly make moment-to-moment adjustments behavior response results actions. Particularly, they neocortex integrate information provided by sensory organs (bottom-up information) with internally generated signals expectations attentional (top-down information). This integration occurs pyramidal cells (PCs) their long apical dendrite, which branches extensively into dendritic tuft layer 1 (L1). The outermost neocortex, L1 is conserved across cortical areas species. Importantly, predominant input for top-down information, relayed rich, dense mesh long-range projections that provide PCs. Here, we discuss recent progress understanding composition review evidence processing contributes functions perception, cross-modal integration, controlling states consciousness, attention, learning.

Язык: Английский

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A transcriptomic axis predicts state modulation of cortical interneurons

Nature, Год журнала: 2022, Номер 607(7918), С. 330 - 338

Опубликована: Июль 6, 2022

Abstract Transcriptomics has revealed that cortical inhibitory neurons exhibit a great diversity of fine molecular subtypes 1–6 , but it is not known whether these have correspondingly diverse patterns activity in the living brain. Here we show primary visual cortex (V1) correlates with brain state, which are organized by single factor: position along main axis transcriptomic variation. We combined vivo two-photon calcium imaging mouse V1 method to identify mRNA for 72 selected genes ex slices. classified imaged layers 1–3 into three-level hierarchy 5 subclasses, 11 types and 35 using previously defined clusters 3 . Responses stimuli differed significantly only between cells Sncg subclass uniformly suppressed, other subclasses predominantly excited. Modulation state at all hierarchical levels could be largely predicted from first principal component, also correlations simultaneously recorded cells. Inhibitory fired more resting, oscillatory states had smaller fraction their axonal projections layer 1, narrower spikes, lower input resistance weaker adaptation as determined vitro 7 expressed cholinergic receptors. Subtypes during arousal opposite properties. Thus, simple principle may explain how shape state-dependent processing.

Язык: Английский

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