Synaptic enrichment and dynamic regulation of the two opposing dopamine receptors within the same neurons

Опубликована: Янв. 20, 2025

Dopamine can play opposing physiological roles depending on the receptor subtype. In fruit fly Drosophila melanogaster , Dop1R1 and Dop2R encode D 1 - 2 -like receptors, respectively, are reported to oppositely regulate intracellular cAMP levels. Here, we profiled expression subcellular localization of endogenous in specific cell types mushroom body circuit. For cell-type-specific visualization proteins, employed reconstitution split-GFP tagged proteins. We detected dopamine receptors at both presynaptic postsynaptic sites multiple types. Quantitative analysis revealed enrichment sites, with showing a greater degree than Dop1R1. The neurons suggests dual feedback regulation as autoreceptors. Furthermore, discovered starvation-dependent, bidirectional modulation PAM PPL1 clusters, two distinct subsets neurons, suggesting appetitive behaviors. Our results highlight significance co-expression spatial conditional responses neurons.

Язык: Английский

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Synaptic enrichment and dynamic regulation of the two opposing dopamine receptors within the same neurons

eLife, Год журнала: 2025, Номер 13

Опубликована: Янв. 30, 2025

Dopamine can play opposing physiological roles depending on the receptor subtype. In fruit fly Drosophila melanogaster , Dop1R1 and Dop2R encode D 1 - 2 -like receptors, respectively, are reported to oppositely regulate intracellular cAMP levels. Here, we profiled expression subcellular localization of endogenous in specific cell types mushroom body circuit. For cell-type-specific visualization proteins, employed reconstitution split-GFP tagged proteins. We detected dopamine receptors at both presynaptic postsynaptic sites multiple types. Quantitative analysis revealed enrichment sites, with showing a greater degree than Dop1R1. The neurons suggests dual feedback regulation as autoreceptors. Furthermore, discovered starvation-dependent, bidirectional modulation protocerebral anterior medial (PAM) posterior lateral (PPL1) clusters, two distinct subsets neurons, suggesting their regulating appetitive behaviors. Our results highlight significance co-expression spatial conditional responses neurons.

Язык: Английский

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Cell-type-specific fluorescent tagging of endogenous target proteins reveals synaptic enrichment and dynamic regulations of dopamine receptors

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 30, 2024

Abstract Dopamine can play opposing physiological roles depending on the receptor subtype. In fruit fly Drosophila melanogaster , Dop1R1 and Dop2R encode D 1 - 2 -like receptors, respectively, are reported to oppositely regulate intracellular cAMP levels. Here, we profiled expression subcellular localization of endogenous in specific cell types mushroom body circuit. For cell-type-specific visualization proteins, employed reconstitution split-GFP tagged proteins. We detected dopamine receptors at both presynaptic postsynaptic sites multiple types. Quantitative analysis revealed enrichment sites, with showing a greater degree than Dop1R1. The neurons suggests dual feedback regulation as autoreceptors. Furthermore, discovered starvation-dependent, bidirectional modulation PAM PPL1 clusters, two distinct subsets neurons, suggesting appetitive behaviors. Our results highlight significance co-expression spatial conditional responses neurons.

Язык: Английский

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Cell-type-specific fluorescent tagging of endogenous target proteins reveals synaptic enrichment and dynamic regulations of dopamine receptors

eLife, Год журнала: 2024, Номер 13

Опубликована: Июль 18, 2024

Dopamine can play opposing physiological roles depending on the receptor subtype. In fruit fly Drosophila melanogaster , Dop1R1 and Dop2R encode D 1 - 2 -like receptors, respectively, are reported to oppositely regulate intracellular cAMP levels. Here, we profiled expression subcellular localization of endogenous in specific cell types mushroom body circuit. For cell-type-specific visualization proteins, employed reconstitution split-GFP tagged proteins. We detected dopamine receptors at both presynaptic postsynaptic sites multiple types. Quantitative analysis revealed enrichment sites, with showing a greater degree than Dop1R1. The neurons suggests dual feedback regulation as autoreceptors. Furthermore, discovered starvation-dependent, bidirectional modulation protocerebral anterior medial (PAM) posterior lateral (PPL1) clusters, two distinct subsets neurons, suggesting their regulating appetitive behaviors. Our results highlight significance co-expression spatial conditional responses neurons.

Язык: Английский

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Serotonin acts through multiple cellular targets during an olfactory critical period.

iScience, Год журнала: 2024, Номер 27(11), С. 111083 - 111083

Опубликована: Окт. 9, 2024

Язык: Английский

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