RMI1 is essential for maintaining rice genome stability at high temperature DOI
Kangwei Liu,

Mengna Wang,

Li Wang

и другие.

The Plant Journal, Год журнала: 2024, Номер 120(5), С. 1735 - 1750

Опубликована: Ноя. 21, 2024

SUMMARY Heat is a critical environmental stress for plant survival. One of its harmful effects on the cells disruption genome integrity. However, mechanisms by which plants cope with heat‐induced DNA damage remain largely unknown. RMI1, component RTR (RECQ4‐TOP3α‐RMI1) complex, plays pivotal role in maintaining stability. In this study, we identified target gene RMI1 characterizing high‐temperature‐sensitive mutant. The growth and development rmi1‐1 seedlings carrying non‐frameshift mutation were hindered at 38°C. Abnormal mitotic chromosome behaviours ultimately led to cell death root tips. Additionally, presence fragments during anaphase I caused pollen abortion sterility plants. Yeast two‐hybrid assays revealed that interactions between RMI1‐1 RECQ4 or TOP3α weakened increasing temperature entirely ceased 36°C. contrast, functional maintained under same conditions. These results indicate disrupts formation complex high temperatures, leading defects repair increased sensitivity heat stress. embryos rmi1‐cr2 mutant frameshift exhibited complete lethality. addition, overexpression enhanced tolerance rice. findings provide insights into molecular responds temperatures stability

Язык: Английский

Mechanistic insights into DNA damage recognition and checkpoint control in plants DOI
Josephine Herbst,

Q. Q. Li,

Lieven De Veylder

и другие.

Nature Plants, Год журнала: 2024, Номер 10(4), С. 539 - 550

Опубликована: Март 19, 2024

Язык: Английский

Процитировано

12
The lowdown on breakdown: Open questions in plant proteolysis DOI Creative Commons
Nancy A. Eckardt, Tamar Avin‐Wittenberg, Diane C. Bassham

и другие.

The Plant Cell, Год журнала: 2024, Номер 36(9), С. 2931 - 2975

Опубликована: Июль 9, 2024

Abstract Proteolysis, including post-translational proteolytic processing as well protein degradation and amino acid recycling, is an essential component of the growth development living organisms. In this article, experts in plant proteolysis pose discuss compelling open questions their areas research. Topics covered include role cell cycle, DNA damage response, mitochondrial function, generation N-terminal signals (degrons) that mark many proteins for (N-terminal acetylation, Arg/N-degron pathway, chloroplast N-degron pathway), developmental metabolic signaling (photomorphogenesis, abscisic strigolactone signaling, sugar metabolism, postharvest regulation), responses to environmental (endoplasmic-reticulum-associated degradation, chloroplast-associated drought tolerance, growth-defense trade-off), functional diversification peptidases. We hope these thought-provoking discussions help stimulate further

Язык: Английский

Процитировано

9
KNO1‐mediated autophagic degradation of the Bloom syndrome complex component RMI1 promotes homologous recombination DOI Creative Commons
Po‐Yu Chen, Nancy De Winne, Geert De Jaeger

и другие.

The EMBO Journal, Год журнала: 2023, Номер 42(10)

Опубликована: Март 27, 2023

Abstract Homologous recombination (HR) is a key DNA damage repair pathway that tightly adjusted to the state of cell. A central regulator homologous conserved helicase‐containing Bloom syndrome complex, renowned for its crucial role in maintaining genome integrity. Here, we show Arabidopsis thaliana , complex activity controlled by selective autophagy. We find recently identified KNO1 facilitates K63‐linked ubiquitination RMI1, structural component thereby triggering RMI1 autophagic degradation and resulting increased recombination. Conversely, reduced makes plants hypersensitive damage. itself also at level proteolysis, this case mediated ubiquitin–proteasome system, becoming stabilized upon via two redundantly acting deubiquitinases, UBP12 UBP13. These findings uncover regulatory cascade interconnected protein steps fine‐tuned HR response

Язык: Английский

Процитировано

15
Cortical determinants of loudness perception and auditory hypersensitivity DOI Creative Commons
Kameron K. Clayton, Matthew McGill, Bshara Awwad

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 30, 2024

Parvalbumin-expressing inhibitory neurons (PVNs) stabilize cortical network activity, generate gamma rhythms, and regulate experience-dependent plasticity. Here, we observed that activation or inactivation of PVNs functioned like a volume knob in the mouse auditory cortex (ACtx), turning neural behavioral classification sound level up down over 20dB range. PVN loudness adjustments were "sticky", such single bout 40Hz stimulation sustainably suppressed ACtx responsiveness, potentiated feedforward inhibition, behaviorally desensitized mice to loudness. Sensory sensitivity is cardinal feature autism, aging, peripheral neuropathy, prompting us ask whether can persistently desensitize with hyperactivity, hypofunction, hypersensitivity triggered by cochlear sensorineural damage. We found 16-minute session restored normal perception for one week, showing perceptual deficits irreversible injuries be reversed through targeted circuit interventions.

Язык: Английский

Процитировано

3
The role ofSMURFsin non‐cancerous diseases DOI Creative Commons
Dong Wang, Yuanming Zou, Xinyue Huang

и другие.

The FASEB Journal, Год журнала: 2023, Номер 37(8)

Опубликована: Июль 25, 2023

Abstract The ubiquitin‐proteasome system is a crucial mechanism for regulating protein levels in cells, with substrate‐specific E3 ubiquitin ligases serving as an integral component of this system. Among these are SMAD‐specific ubiquitin‐protein ligase 1 (SMURF1) and 2 (SMURF2), which belong to the neural precursor cell‐expressed developmentally downregulated 4 (NEDD4) subfamily Homologous E6‐AP COOH terminus (HECT)‐type ligases. As ligases, SMURFs have critical functions stability multiple proteins, thereby maintaining physiological processes such cell migration, proliferation, apoptosis. occurrence many diseases attributed abnormal physiology imbalance homeostasis. It noteworthy that play pivotal roles disease progression, regulatory being complex either facilitative or inhibitory. In review, we elucidate mechanisms by SMURF1 SMURF2 can regulate progression non‐cancerous diseases. These significant findings offer potential novel therapeutic targets various new avenues research on SMURF proteins.

Язык: Английский

Процитировано

8
Insights into a functional model of key deubiquitinases UBP12/13 in plants DOI Open Access
Shiqi Zhang, Ningning Hu, Feifei Yu

и другие.

New Phytologist, Год журнала: 2024, Номер 242(2), С. 424 - 430

Опубликована: Фев. 26, 2024

Understanding the complexities of protein ubiquitination is crucial, as it plays a multifaceted role in controlling stability, activity, subcellular localization, and interaction, which are central to diverse biological processes. Deubiquitinases (DUBs) serve reverse ubiquitination, but research progress plant DUBs noticeably limited. Among existing studies, UBIQUITIN-SPECIFIC PROTEASE 12 (UBP12) UBP13 have garnered attention for their extensive processes plants. This review systematically summarizes recent advancements UBP12/13 emphasizing function, substrate specificity, relationship with E3 ubiquitin ligases, similarities differences mammalian orthologue, USP7. By unraveling molecular mechanisms UBP12/13, this offers in-depth insights into ubiquitin-proteasome system (UPS) plants aims catalyze further explorations comprehensive understanding field.

Язык: Английский

Процитировано

3
Erasing marks: Functions of plant deubiquitylating enzymes in modulating the ubiquitin code DOI
Karin Vogel,

Erika Isono

The Plant Cell, Год журнала: 2024, Номер 36(9), С. 3057 - 3073

Опубликована: Апрель 24, 2024

Plant cells need to respond environmental stimuli and developmental signals accurately promptly. Ubiquitylation is a reversible posttranslational modification that enables the adaptation of cellular proteostasis internal or external factors. The different topologies ubiquitin linkages serve as structural basis for code, which can be interpreted by ubiquitin-binding proteins readers in specific processes. ubiquitylation status target regulated ubiquitylating enzymes writers, well deubiquitylating (DUBs) erasers. DUBs remove molecules from proteins. Arabidopsis (A. thaliana) belong 7 protein families exhibit wide range functions play an important role regulating selective degradation processes, including proteasomal, endocytic, autophagic degradation. also shape epigenetic landscape modulate DNA damage repair In this review, we summarize current knowledge on plants, their functions, molecular mechanisms involved regulation plant DUBs.

Язык: Английский

Процитировано

2
The Alexander Disease Protein GFAP Drives Mitochondrial Fission DOI Creative Commons
Ding Xiong, Linghai Kong,

Ye Sing Tan

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 21, 2024

Abstract Mitochondrial plasticity, coordinated by fission and fusion, is crucial to ensure cellular functions. mediated the GTPase Drp1 at constriction site, which proposed be driven actin-myosin contractile force. However, mechanism that propels remains unclear, potential involvement of additional mechanisms in this process an open question. Here, using structured illumination microscopy electron microscopy, we show type-III intermediate filament glial fibrillary acidic protein (GFAP) closely surrounds mitochondria sites associates with accumulated molecules. Remarkably, loss GFAP results hyperfused under physiological condition even Ca 2+ -induced mitochondrial fission. Additionally, mutations GFAP, cause Alexander disease, result elevated recruitment leading significantly increased fissions. Taking together, these findings suggest a novel division filaments.

Язык: Английский

Процитировано

0
Phosphorylation of KRP3, a KIP-related protein by MPK3 modulates rice tiller and seed number by fine-tuning cell division DOI Open Access
Gopal Banerjee, Sarvesh Jonwal, Uttam Pal

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 19, 2024

Abstract During the cell cycle process, multiple inhibitors function as checkpoint regulators ensuring an impeccable duplication of genetic material. Kip-Related Proteins (KRPs) are a group plant-specific and known to regulate plant architecture yield by differentially regulating division. KRPs, though functionally conserved, dynamic in their amino acid composition. Interestingly, some KRPs specific dicots, for monocots. In this study, we have identified that presence KRP3 KRP6 is Poaceae family An in-depth study showed strict regulation its expression actively dividing cells during G1-S phase progression Our phosphorylation target MPK3. The enhances protein stability, which leads strong inhibition proliferation. By generating knock-out lines krp3 , mpk3 double krp3mpk3 our demonstrated MPK3-KRP3 module regulates rice root shoot development well tiller seed numbers. It was observed rate division dose-dependent manner. nutshell, establishes role important regulator vigor yield.

Язык: Английский

Процитировано

0
Crowder induced conformational fluctuations modulate the phase separation of yeast SUP35 NM domain DOI Creative Commons
Sumangal Roychowdhury, Sneha Menon, Narattam Mandal

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 29, 2024

Abstract Intrinsically disordered proteins (IDPs) like Sup35NM can undergo liquid-liquid phase separation (LLPS) to form biomolecular condensates, a process influenced by their conformational flexibility and the crowded intracellular environment. This study investigates how molecular crowding, specifically size shape of crowders Dextran Ficoll, modulates states behavior Sup35NM. Using fluorescence correlation spectroscopy (FCS) dynamics simulations, we observed that Dextran, depending on its weight, induces both compaction expansion Sup35NM, driving at certain thresholds. Notably, rod-like promote separation, while spherical Ficoll does not, highlighting impact crowder geometry IDP behavior. Computational modelling further revealed influences Sup35NM’s ensemble modulating intra- inter-domain interactions. These findings elucidate role crowding agents in behavior, suggesting cellular may regulate functionality through control.

Язык: Английский

Процитировано

0