Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Май 26, 2025
Abstract
We
investigated
the
role
and
mechanism
of
hsa-HLA-DRB1
in
development
progression
diabetic
foot
ulcers.
High-throughput
sequencing
was
performed
on
three
normal
trauma
tissues
ulcer
tissues.
The
circRNAs
with
significant
differences
were
identified.
downstream
miRNAs
predicted
by
miRanda
RNAhybrid
databases,
mRNAs
TargetScan
database.
Validation
CCK8,
flow
cytometry,
trabecular
scratch
assay,
tubule
generation
Western
blot,
dual
luciferase
RT-qPCR.
identified
461
significantly
different
circRNAs,
which
260
up-regulated
201
down-regulated.
Compared
to
tissue,
highly
expressed
hsa-HLA-DRB1/miRNA_12118/FLT-1
axis
constructed.
In
vitro,
we
found
that
HLA-DRB1
overexpression
inhibited
cell
viability,
wound
healing,
formation,
promoted
apoptosis,
enhanced
FLT-1
expression
HUVECs.
upregulation
may
promote
hsa
-
HLA
DRB1
inhibit
biological
function
endothelial
cells
targeting
miRNA_12118
acting
FLT
−
1.
FLT-1.
Therefore,
our
study
highlights
key
trauma.
The
progression
and
metastasis
of
breast
cancer
patients
are
regulated
by
genetics
epigenetics.
Circular
RNA
(circRNA)
plays
a
pivotal
role
in
modulating
the
advancement
tumors.
study
aimed
to
explore
clinical
performance
regulatory
hsa-circVIM
its
modulatory
effect
on
tamoxifen
resistance
hormone
receptor
(HR)
positive
cells.
RT-qPCR
was
performed
detect
expression
tissues
cells.CCK-8
assay,
Transwell
flow
cytometric
analyses
were
evaluate
effects
cellular
activities
cells
TAM
sensitivity
MCF7/TR
Bioinformatic
conducted
make
function
pathway
enrichment
analyses.
raised
predicted
unsatisfactory
overall
survival
outcomes.
Silencing
suppressed
cell
viability,
migration
capacities,
while
simultaneously
enhancing
inducing
apoptosis
HR-positive
targeting
miR-1294.
Elevated
suggested
potential
as
prognostic
biomarker.
functions
both
cancer-promoting
molecule
regulator
responsiveness
regulating
miR-1294
expression.
Therefore,
serves
potent
biomarker
for
prognosis,
promoting
progression,
may
offer
therapeutic
avenue
overcome
resistance.
BIO Web of Conferences,
Год журнала:
2025,
Номер
174, С. 01018 - 01018
Опубликована: Янв. 1, 2025
As
a
prevalent
metabolic
disorder
arising
in
pregnancy,
gestational
diabetes
mellitus
(GDM)
poses
substantial
risks
to
maternal
and
fetal
well-being.
The
development
of
GDM
is
substantially
influenced
by
hereditary
components,
with
variations
observed
across
different
racial
groups.
Genome
wide
association
studies
(GWAS)
are
an
effective
tool
that
provides
new
insights
into
the
genetic
mechanism
GDM.
This
review
details
current
application
GWAS
research.
It
sums
up
identified
susceptibility
gene
loci
their
potential
functions
Chinese
population
Moreover,
it
underscores
importance
independent
By
establishing
relatively
comprehensive
reference
framework,
this
study
intends
enhance
exploration
basis
GDM,
improve
capacity
precise
risk
assessment,
develop
customized
treatment
strategies
clinical
context.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(10), С. 4842 - 4842
Опубликована: Май 19, 2025
Circular
RNAs
(circRNAs)
are
increasingly
recognized
as
significant
regulators
in
multiple
cancers,
such
hepatocellular
carcinoma
(HCC),
frequently
affecting
microRNA
(miRNA)
expression.
The
diagnostic
and
prognostic
roles
of
circRNAs,
specifically
circ_0009910
circ_0027478,
conjunction
with
miR-1236-3p,
HCC,
have
not
yet
been
fully
investigated.
In
this
pilot
study,
we
assessed
the
expression
levels
circ_0009910,
miR-1236-3p
plasma
samples
from
100
patients
diagnosed
HCC
50
healthy
controls
through
reverse
transcriptase–quantitative
polymerase
chain
reaction
(RT-qPCR).
performance
was
evaluated
using
receiver
operating
characteristic
(ROC)
curve
analysis,
correlations
clinicopathological
features
were
examined.
Circ_0009910
circ_0027478
exhibited
upregulation
(p
<
0.05),
whereas
demonstrated
downregulation
0.05).
accuracy
(area
under
[AUC]
=
0.90),
effectively
differentiating
showing
a
correlation
tumor
size,
metastasis,
alpha-fetoprotein
(AFP)
Both
positive
features,
an
inverse
correlation.
Logistic
regression
validated
capabilities
these
biomarkers.
results
indicate
that
AFP
three,
present
promising
profile
for
HCC.
Additional
validation
larger
cohorts
is
required
to
establish
their
clinical
utility.
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Май 26, 2025
Abstract
We
investigated
the
role
and
mechanism
of
hsa-HLA-DRB1
in
development
progression
diabetic
foot
ulcers.
High-throughput
sequencing
was
performed
on
three
normal
trauma
tissues
ulcer
tissues.
The
circRNAs
with
significant
differences
were
identified.
downstream
miRNAs
predicted
by
miRanda
RNAhybrid
databases,
mRNAs
TargetScan
database.
Validation
CCK8,
flow
cytometry,
trabecular
scratch
assay,
tubule
generation
Western
blot,
dual
luciferase
RT-qPCR.
identified
461
significantly
different
circRNAs,
which
260
up-regulated
201
down-regulated.
Compared
to
tissue,
highly
expressed
hsa-HLA-DRB1/miRNA_12118/FLT-1
axis
constructed.
In
vitro,
we
found
that
HLA-DRB1
overexpression
inhibited
cell
viability,
wound
healing,
formation,
promoted
apoptosis,
enhanced
FLT-1
expression
HUVECs.
upregulation
may
promote
hsa
-
HLA
DRB1
inhibit
biological
function
endothelial
cells
targeting
miRNA_12118
acting
FLT
−
1.
FLT-1.
Therefore,
our
study
highlights
key
trauma.
