Translational Oncology,
Год журнала:
2024,
Номер
41, С. 101860 - 101860
Опубликована: Янв. 22, 2024
Nutrient
restriction
in
cancer
cells
can
activate
a
number
of
stress
response
pathways
for
cell
survival.
We
aimed
to
determine
mechanistically
how
nutrient
depletion
colorectal
(CRC)
leads
cellular
adaptation.
Cell
survival
under
(ND)
was
evaluated
by
colony
formation
and
vivo
tumor
assays.
Lysosomes
are
activated
with
ND;
therefore,
we
incubated
the
ND
V-ATPase
inhibitor
Bafilomycin
A1
(ND+Baf).
The
expression
epithelial
mesenchymal
markers
ND+Baf
determined
RNA
sequencing
RT-qPCR
while
motility
an
Chorioallantoic
membrane
(CAM)
assay.
Reorganization
cytoskeletal
network
lysosomal
positioning
immunocytochemistry.
4
different
lines
showed
high
viability,
forming
ability
increased
one
or
more
markers,
suggesting
activation
partial
(p)-EMT.
observed
further
increase
p-EMT
numerous
protrusions,
decreased
cell-cell
adhesion
3D,
cells.
protrusions
were
primarily
mediated
microtubules
enabled
relocalization
lysosomes
from
perinuclear
region
periphery.
CRC
cells,
which
exacerbated
alkalinization.
also
containing
lysosomes,
may
lead
exocytosis
enhanced
motility.
Biomolecules,
Год журнала:
2023,
Номер
13(11), С. 1614 - 1614
Опубликована: Ноя. 4, 2023
The
skin
is
the
most-extensive
and
-abundant
tissue
in
human
body.
Like
many
organs,
as
we
age,
experiences
gradual
atrophy
both
epidermis
dermis.
This
can
be
primarily
attributed
to
diminishing
population
of
epidermal
stem
cells
reduction
collagen,
which
primary
structural
protein
alterations
occurring
dermis
due
aging
process
result
disruptions
structure
functionality
skin.
creates
a
microenvironment
conducive
age-related
conditions
such
compromised
barrier,
slowed
wound
healing,
onset
cancer.
review
emphasizes
recent
molecular
discoveries
related
evaluates
preventive
approaches,
use
topical
retinoids.
Topical
retinoids
have
demonstrated
promise
enhancing
texture,
fine
lines,
augmenting
thickness
dermal
layers.
Journal of Neuroinflammation,
Год журнала:
2024,
Номер
21(1)
Опубликована: Авг. 8, 2024
Astrocytes
respond
and
contribute
to
neuroinflammation
by
adopting
inflammatory
reactive
states.
Although
recent
efforts
have
characterized
the
gene
expression
signatures
associated
with
these
states,
cell
biology
underlying
astrocyte
phenotypes
remains
under-explored.
Here,
we
used
CRISPR-based
screening
in
human
iPSC-derived
astrocytes
identify
mTOR
activation
a
driver
of
cytokine-induced
endolysosomal
system
remodeling,
manifesting
as
alkalinization
compartments,
decreased
autophagic
flux,
increased
exocytosis
certain
cargos.
Through
proteomics,
identified
focused
on
one
such
cargo-IL-32,
disease-associated
pro-inflammatory
cytokine
not
present
rodents,
whose
secretion
mechanism
is
well
understood.
We
found
that
IL-32
was
partially
secreted
extracellular
vesicles
likely
be
exosomes.
Furthermore,
involved
polarization
states
upregulated
multiple
sclerosis
lesions.
believe
our
results
advance
understanding
biological
pathways
potential
therapeutic
targets.
Cells,
Год журнала:
2025,
Номер
14(3), С. 183 - 183
Опубликована: Янв. 24, 2025
Lysosomes
in
mammalian
cells
are
recognized
as
key
digestive
organelles,
containing
a
variety
of
hydrolytic
enzymes
that
enable
the
processing
both
endogenous
and
exogenous
substrates.
These
organelles
digest
various
macromolecules
recycle
them
through
autophagy–lysosomal
system.
Recent
research
has
expanded
our
understanding
lysosomes,
identifying
not
only
centers
degradation
but
also
crucial
regulators
nutrient
sensing,
immunity,
secretion,
other
vital
cellular
functions.
The
lysosomal
pathway
plays
significant
role
vascular
regulation
is
implicated
diseases
such
atherosclerosis.
During
atherosclerotic
plaque
formation,
macrophages
initially
engulf
large
quantities
lipoproteins,
triggering
pathogenic
responses
include
dysfunction,
foam
cell
subsequent
atherosclerosis
development.
Lysosomal
along
with
inefficient
apoptotic
accumulation
modified
low-density
negatively
impacts
lesion
progression.
studies
have
highlighted
dysfunction
contributes
critically
to
cell-
stage-specific
manner.
In
this
review,
we
discuss
mechanisms
biogenesis
its
regulatory
lesions.
Based
on
these
functions,
propose
targeting
lysosomes
could
offer
novel
therapeutic
approach
for
atherosclerosis,
shedding
light
connection
between
disease
progression
while
offering
new
insights
into
potential
anti-atherosclerotic
strategies.
ABSTRACT
The
establishment
of
various
molecular,
physiological,
and
genetic
markers
for
cellular
senescence
aging‐associated
conditions
has
progressed
the
aging
study.
To
identify
such
markers,
a
combination
optical,
proteomic‐,
sequencing‐based
tools
is
primarily
used,
often
accompanying
extrinsic
labels.
Yet,
clinical
detection
at
cellular,
tissue
levels
are
still
lacking
which
profoundly
hinders
advancements
in
specific
timely
prevention
aging‐related
diseases
pathologies.
Raman
spectroscopy,
with
its
capability
rapid,
label‐free,
non‐invasive
analysis
molecular
compositions
alterations
cells
tissues,
holds
considerable
promise
vivo
applications.
In
this
review,
we
present
recent
application
spectroscopy
to
study
tissues.
We
explore
use
related
techniques
detecting
senescence,
focusing
on
that
accompany
these
processes.
Subsequently,
provide
review
identifying
changes
molecules
within
tissues
organs.
Journal of Parkinson s Disease,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 2, 2025
Parkinson's
disease
(PD)
is
the
most
common
neurodegenerative
movement
disorder,
affecting
1–2%
of
people
over
age
65.
The
risk
developing
PD
dramatically
increases
with
advanced
age,
indicating
that
aging
likely
a
driving
factor
in
neuropathogenesis.
Several
age-associated
biological
changes
are
also
hallmarks
neuropathology,
including
mitochondrial
dysfunction,
oxidative
stress,
and
neuroinflammation.
Accumulation
senescent
cells
an
important
feature
contributes
to
age-related
diseases.
How
cellular
senescence
affects
brain
health
whether
this
phenomenon
neuropathogenesis
not
yet
fully
understood.
In
review,
we
highlight
aging,
loss
proteostasis,
genomic
instability
telomere
attrition
relation
well
established
neuropathological
pathways.
We
then
discuss
context
neuroscience
review
studies
directly
examine
PD.
Studying
presents
challenges
holds
promise
for
advancing
our
understanding
mechanisms,
which
could
contribute
development
effective
disease-modifying
therapeutics.
Targeting
or
modulating
senescence-associated
secretory
phenotype
(SASP)
requires
comprehensive
complex
relationship
between
pathogenesis
senescence.
