A defined diet for pre-adult Drosophila melanogaster
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Март 23, 2024
Abstract
Drosophila
melanogaster
is
unique
among
animal
models
because
it
has
a
fully
defined
synthetic
diet
available
to
study
nutrient-gene
interactions.
However,
use
of
this
limited
adult
studies
due
impaired
larval
development
and
survival.
Here,
we
provide
an
adjusted
formula
that
reduces
the
developmental
period,
restores
fat
levels,
enhances
body
mass,
rescues
survivorship
without
compromise
lifespan.
To
demonstrate
application
formula,
explored
pre-adult
compositions
therapeutic
potential
in
model
inherited
metabolic
disorder
affecting
metabolism
branched-chain
amino
acids.
We
reveal
rapid,
specific,
predictable
nutrient
effects
on
disease
state
consistent
with
observations
from
mouse
patient
studies.
Together,
our
provides
powerful
means
which
examine
interplay
between
across
all
life
stages
model.
Язык: Английский
The Three-Dimensional Structure of the Genome of the Dark Septate Endophyte Exophiala tremulae and Its Symbiosis Effect on Alpine Meadow Plant Growth
Journal of Fungi,
Год журнала:
2025,
Номер
11(4), С. 246 - 246
Опубликована: Март 24, 2025
The
establishment
of
artificial
grassland
is
a
good
pathway
for
resolving
serious
social
and
economic
problems
in
the
Qinghai–Tibet
Plateau.
Some
beneficial
indigenous
microbes
may
be
used
to
improve
productivity
grassland.
genome
dark
septate
fungus,
Exophiala
tremulae
CICC2537,
was
sequenced
assembled
at
chromosome
level
using
PacBio
sequencing
platform,
with
assistance
Hi-C
technique
scaffolding,
its
3D
structures
were
investigated.
size
E.
51.903848
Mb,
it
contains
eight
chromosomes.
A
total
12,277
protein-coding
genes
predicted,
11,932
(97.19%)
annotated.
As
distribution
exon
intron
number
gene
GC
CDS
GC,
showed
similar
patterns
other
investigated
members
genus
Exophiala.
analysis
carbohydrate-active
enzymes
that
possesses
greatest
auxiliary
activities
lowest
carbohydrate-binding
modules
among
fungi.
candidate
effector
proteins
3337,
out
which
cytoplasmic
apoplastic
made
up
3100
163,
respectively.
whole
contained
40
compartment
76
Bs,
there
no
significant
difference
content
Bs.
predicted
contain
155
topologically
associating
domains
(TADs),
their
average
length
250,000
bp,
but
differences
numbers
per
bin
localized
within
boundaries
interiors
TADs.
Comparative
diverged
from
mesophila
about
34.1
(30.0–39.1)
Myr
ago,
calicioides
85.6
(76.1–90.6)
ago.
Compared
all
fungi,
contraction
expansion
families
13
89,
respectively,
14
670,
Our
work
provides
basis
use
fungus
alpine
further
research
into
symbiosis
mechanisms,
growth
plant
species
Язык: Английский
Patterns of protein synthesis in the budding yeast cell cycle: variable or constant?
Microbial Cell,
Год журнала:
2024,
Номер
11, С. 321 - 327
Опубликована: Авг. 20, 2024
Abstract
Proteins
are
the
principal
macromolecular
constituent
of
proliferating
cells,
and
protein
synthesis
is
viewed
as
a
primary
metric
cell
growth.
While
there
celebrated
examples
proteins
whose
levels
periodic
in
cycle
(e.g.,
cyclins),
concentration
most
was
not
thought
to
change
cycle,
but
some
recent
results
challenge
this
notion.
The
‘bulk’
focus
article,
specifically
rate
its
synthesis,
budding
yeast
Saccharomyces
cerevisiae
.
Язык: Английский
Branched‐chain amino acid synthesis is coupled to TOR activation early in the cell cycle in yeast
EMBO Reports,
Год журнала:
2023,
Номер
24(9)
Опубликована: Июль 27, 2023
Abstract
How
cells
coordinate
their
metabolism
with
division
determines
the
rate
of
cell
proliferation.
Dynamic
patterns
metabolite
synthesis
during
cycle
are
unexplored.
We
report
first
isotope
tracing
analysis
in
synchronous,
growing
budding
yeast
cells.
Synthesis
leucine,
a
branched‐chain
amino
acid
(BCAA),
increases
through
G1
phase
cycle,
peaking
later
DNA
replication.
Cells
lacking
Bat1,
mitochondrial
aminotransferase
that
synthesizes
BCAAs,
grow
slower,
smaller,
and
delayed
phase,
phenocopying
which
growth‐promoting
kinase
complex
TORC1
is
moderately
inhibited.
Loss
Bat1
lowers
levels
BCAAs
reduces
activity.
Exogenous
provision
valine
and,
to
lesser
extent,
leucine
promotes
division.
Valine
addition
also
In
wild‐type
cells,
activity
dynamic
starting
low
early
but
increasing
cycle.
These
results
suggest
link
between
BCAA
from
glucose
activation
Язык: Английский
TOR Complex 1: Orchestrating Nutrient Signaling and Cell Cycle Progression
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(21), С. 15745 - 15745
Опубликована: Окт. 30, 2023
The
highly
conserved
TOR
signaling
pathway
is
crucial
for
coordinating
cellular
growth
with
the
cell
cycle
machinery
in
eukaryotes.
One
of
two
complexes
budding
yeast,
TORC1,
integrates
environmental
cues
and
promotes
growth.
While
cells
grow,
they
need
to
copy
their
chromosomes,
segregate
them
mitosis,
divide
all
components
during
cytokinesis,
finally
physically
separate
mother
daughter
start
a
new
apart
from
each
other.
To
maintain
size
homeostasis
chromosome
stability,
it
that
mechanisms
control
are
connected
coordinated
cycle.
Successive
periods
high
low
TORC1
activity
would
participate
adequate
progression.
Here,
we
review
known
molecular
through
which
regulates
yeast
Saccharomyces
cerevisiae
have
been
extensively
used
as
model
organism
understand
role
its
mammalian
ortholog,
mTORC1.
Язык: Английский