Swine clones: potential application for animal production and animal models

Animal Reproduction, Год журнала: 2025, Номер 22(1)

Опубликована: Янв. 1, 2025

Abstract Somatic cell nuclear transfer (SCNT), or cloning, is used to reprogram cells and generate genetically identical embryos animals. However, the cloning process inefficient, limiting its application producing valuable In swine, mainly utilized produce modified Indeed, recombinant DNA technologies have evolved considerably in recent years, with homologous recombination gene editing becoming more efficient capable of recombining both alleles a single cell. The selection appropriate their use as donors for SCNT most common method generating edited animals commercial research purposes. This article reviews current applications swine shares our personal experiences procedure this species.

Язык: Английский

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Potentials, prospects and applications of genome editing technologies in livestock production

Saudi Journal of Biological Sciences, Год журнала: 2021, Номер 29(4), С. 1928 - 1935

Опубликована: Ноя. 24, 2021

In recent years, significant progress has been achieved in genome editing applications using new programmable DNA nucleases such as zinc finger (ZFNs), transcription activator-like endonucleases (TALENs) and the clustered regularly interspaced short palindromic repeats/Cas9 system (CRISPR/Cas9). These tools are capable of nicking precisely by targeting specific sequences, enable addition, removal or substitution nucleotides via double-stranded breakage at genomic loci. CRISPR/Cas system, one most tools, affords ability to efficiently generate multiple nicks single experiment. Moreover, systems relatively easy cost effective when compared other technologies. This is part because rely on RNA-DNA binding, unlike that protein-DNA interactions, which higher flexibility more fidelity. Genome have significantly contributed different aspects livestock production disease resistance, improved performance, alterations milk composition, animal welfare biomedicine. However, despite these contributions future potential, technologies also inherent risks, therefore, ethics social acceptance crucial factors associated with implementation review emphasizes impact development breeding numerous species cattle, pigs, sheep goats. discusses mechanisms behind technologies, their potential applications, risks should be considered context livestock.

Язык: Английский

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Genome editing: An insight into disease resistance, production efficiency, and biomedical applications in livestock

Functional & Integrative Genomics, Год журнала: 2024, Номер 24(3)

Опубликована: Май 6, 2024

Язык: Английский

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An overview of recent technological developments in bovine genomics

Veterinary and Animal Science, Год журнала: 2024, Номер 25, С. 100382 - 100382

Опубликована: Июль 23, 2024

Cattle are regarded as highly valuable animals because of their milk, beef, dung, fur, and ability to draft. The scientific community has tried a number strategies improve the genetic makeup bovine germplasm. To ensure higher returns for dairy beef industries, researchers face greatest challenge in improving commercially important traits. One biggest developments last few decades creation instruments cattle improvement is discovery genome. Breeding livestock being revolutionized by genomic selection made possible availability medium- high-density single nucleotide polymorphism (SNP) arrays coupled with sophisticated statistical techniques. It becoming easier access high-dimensional data cattle. Continuously declining genotyping costs an increase services that use return on investment have both significant contribution this. field genomics come long way thanks groundbreaking discoveries such radiation-hybrid mapping, situ hybridization, synteny analysis, somatic cell genetics, cytogenetic maps, molecular markers, association studies quantitative trait loci, high-throughput SNP genotyping, whole-genome shotgun sequencing genome editing. These advancements had positive impact genomics. This manuscript aimed review recent advances technologies breeding future prospects this field.

Язык: Английский

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The public you want, the public you get: Exploring the relationship between the public and science in the debate on xenotransplantation

Public Understanding of Science, Год журнала: 2024, Номер 33(8), С. 961 - 977

Опубликована: Март 4, 2024

The debate that followed the first-in-human cardiac transplantation of a genetically modified pig organ emerged as discussion social justice when patient's criminal record was revealed. This article aims to make sense this by understanding role 'public' today, particularly in relation governance biotechnology. relationship between public and science is increasingly mediated through citizen participation. However, unfolded on matters can be seen an unmediated discourse, which carries risk producing unpredictable outcomes. content gains significance due functional differentiation society. medical subsystem does not consider history terms their involvement legal sphere, is, record. Nevertheless, normative judgements are transferred across systems, allowing for influence opinion potential scorn.

Язык: Английский

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Establishment of a male fertility prediction model with sperm RNA markers in pigs as a translational animal model

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Год журнала: 2022, Номер 13(1)

Опубликована: Июль 7, 2022

Male infertility is an important issue that causes low production in the animal industry. To solve male fertility crisis industry, prediction of sperm quality most step. Sperm RNA potential marker for prediction. We hypothesized expression functional genes related to fertilization will be best target markers. investigate optimum marker, we compared level and a wide range field data acquired from artificial insemination boar semen.Among acrosomal vesicle exocytosis sperm-oocyte fusion, equatorin (EQTN), zona pellucida sperm-binding protein 4 (ZP4), acrosome membrane-associated 3 exhibited high accuracy (70%, 90%, 70%, respectively) as markers evaluate fertility. Combinations EQTN-ZP4, ZP4-protein unc-13 homolog B, ZP4-regulating synaptic membrane 1 (RIMS1) showed highest value, all these are involved reaction.The EQTN-ZP4 model was efficient clustering high-fertility group may useful selection has superior livestock Compared model, ZP4-RIMS1 more low-fertility diagnosis humans other animals. The appointed translational established biomarker combination can widely used various scientific fields such biomedical science.

Язык: Английский

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25 years after Dolly: Update on long‐term effects of embryo biotechnologies

Reproduction in Domestic Animals, Год журнала: 2023, Номер 58(4), С. 473 - 480

Опубликована: Янв. 16, 2023

Since the announcement of birth Dolly, world's first mammal produced by cloning, it was demonstrated for time that somatic cells could be reprogrammed to produce a whole individual. This represented considerable change in paradigm field embryo technologies both humans and animals which led an intense burst research on nuclear transfer but also establishment pluripotency directed edition genome. As such, induced pluripotent gene editing tools, best known being CRISPR-Cas9, are now available scientific community. Nevertheless, cloning associated with important developmental abnormalities variable proportion pregnancies, raising concern about long-term effects at when concept origins health disease had emerged, together better understanding underlying epigenetic modifications. The focus this article is review current knowledge artificial reproduction mammals, leading globally reassuring information although differences present caution remains necessary taking increasing number vitro-produced ruminant equine embryos into account their potential intergenerational consequences.

Язык: Английский

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Regulatory Policies of Genome Editing Products around the World

Journal of Biomedical Research & Environmental Sciences, Год журнала: 2023, Номер 4(10), С. 1447 - 1454

Опубликована: Окт. 1, 2023

Genome editing is one of the powerful tools that has been applied to improvement plants, animals and microorganisms, human therapy. The regulation genome products vary among nations. Here we summarize legislation in different nations let people know how have regulated Additionally, departments responsible for each nation are provided this review.

Язык: Английский

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The Prospect and Application Research Progress of Precision Gene Editing Technology Based on CRISPR/Cas9 in Livestock Genetics and Breeding

Опубликована: Дек. 15, 2023

With the continuous update of gene editing technology, great progress has been made in livestock breeding, researchers have produced edited pigs, cattle, sheep and other improved livestock. Gene technology is a genetic means to knock in, out, delete, inhibit, activate or replace specific bases DNA RNA sequences at genome level achieve accurate modification, can edit genes fixed point without need for templates. In recent years, although regular clusters interspaced short palindromic repeats (CRISPR)/Cas9 system mediated widely used animal breeding research, but so far, system's precise insertion efficiency foreign not high enough, there are certain off-target effects, it enough be large such as cashmere goats. this paper, development status, challenges, application prospects look into distance CRISPR/ Cas9-mediated precisiongene were reviewed, order provide theoretical reference function analysis, improvement with local economic characteristics.

Язык: Английский

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Dolly at 25… is she ‘… still goin’ strong?’

Reproduction, Год журнала: 2021, Номер 162(1), С. E1 - E3

Опубликована: Июнь 21, 2021

Язык: Английский

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