Light regulation of secondary metabolism in fungi

Journal of Biological Engineering, Год журнала: 2023, Номер 17(1)

Опубликована: Авг. 31, 2023

Abstract Fungi have evolved unique metabolic regulation mechanisms for adapting to the changing environments. One of key features fungal adaptation is production secondary metabolites (SMs), which are essential survival and beneficial organism. Many these SMs produced in response environmental cues, such as light. In all species studied, Velvet complex transcription factor VeA a central player light regulatory network. addition growth development, intensity wavelength affects formation broad range metabolites. Recent studies, mainly on genus Aspergillus , revealed that dimer VeA-VelB LaeA does not only regulate gene expression light, but can also be involved regulating SMs. Furthermore, complexes wide effect different types this review, we discussed role metabolism. addition, reviewed photoreceptors, factors, signaling pathways light-dependent The effects factors metabolites, well potential applications pharmaceuticals other products were discussed. Finally, provided an overview current research field suggested areas future research.

Язык: Английский

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The phosducin-like protein PhLP1 impacts regulation of glycoside hydrolases and light response in Trichoderma reesei

BMC Genomics, Год журнала: 2011, Номер 12(1)

Опубликована: Дек. 1, 2011

Abstract Background In the biotechnological workhorse Trichoderma reesei ( Hypocrea jecorina ) transcription of cellulase genes as well efficiency secreted mixture are modulated by light. Components heterotrimeric G-protein pathway interact with light-dependent signals, rendering this a key regulator gene expression. Results As regulators signaling, class I phosducin-like proteins, assumed to act co-chaperones for beta-gamma folding and exert their function in response light higher eukaryotes. Our results revealed responsive T. protein phlp1 indicate dependent PhLP1 also fungi. We showed functions PhLP1, GNB1 GNG1 same pathway, one major output being regulation glycoside hydrolase including . found no direct correlation between growth rate global hydrolases, which suggests that does not occur only at level substrate degradation efficiency. Additionally, all important proper responsiveness during long term exposure. absence, amount regulated increased from 2.7% wild type 14% Δ Besides degradative enzymes, was impact on involved sexual development, accordance decreased fruiting body formation The lack drastically diminished ascospore discharge Conclusions is crucial interconnection nutrient signaling , acting nodes, influence responsiveness, development.

Язык: Английский

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Development stage-specific proteomic profiling uncovers small, lineage specific proteins most abundant in the Aspergillus Fumigatus conidial proteome

Proteome Science, Год журнала: 2012, Номер 10(1)

Опубликована: Апрель 30, 2012

The pathogenic mold Aspergillus fumigatus is the most frequent infectious cause of death in severely immunocompromised individuals such as leukemia and bone marrow transplant patients. Germination inhaled conidia (asexual spores) host critical for initiation infection, but little known about underlying mechanisms this process.To gain insights into early germination events facilitate identification potential stage-specific biomarkers vaccine candidates, we have used quantitative shotgun proteomics to elucidate patterns protein abundance changes during fungal development. Four different stages were examined: dormant conidia, isotropically expanding hyphae which germ tube emergence has just begun, pre-septation hyphae. To enrich glycan-linked cell wall proteins an alkaline extraction method. Shotgun proteomic resulted 375 unique gene products with high confidence, no evidence enrichment wall-immobilized secreted proteins. interesting discovery was 52 enriched including 28 that never been detected A. conidial proteome signaling Pil1, chaperones BipA calnexin, transcription factor HapB. Additionally found many small, specific unknown function 17 hypothetical Thus, abundant protein, Grg1 (AFUA_5G14210), also one smallest study (M.W. 7,367). Among previously characterized melanin pigment pseurotin A biosynthesis enzymes, histones H3 H4.1, other involved conidiation response oxidative or hypoxic stress. In contrast, tubes, samples responsible housekeeping functions, particularly translation, respiratory metabolism, amino acid carbohydrate biosynthesis, tricarboxylic cycle.The observed temporal expression suggest are dominated by lineage-specific Some them may play key roles host-pathogen interactions, signal transduction germination, survival hostile environments.

Язык: Английский

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Seeing the world differently: variability in the photosensory mechanisms of two model fungi

Environmental Microbiology, Год журнала: 2015, Номер 18(1), С. 5 - 20

Опубликована: Сен. 16, 2015

Light plays an important role for most organisms on this planet, serving either as a source of energy or information the adaptation biological processes to specific times day. The fungal kingdom is estimated contain well over million species, possibly 10-fold more, and it that majority fungi respond light, eliciting changes in several physiological characteristics including pathogenesis, development secondary metabolism. Two model photobiological studies have taken centre-stage last few decades--Neurospora crassa Aspergillus nidulans. In review, we will first discuss our understanding light response N. crassa, about which known, then juxtapose with A. nidulans, which, be described below, provides excellent template photosensory cross-talk. Finally, end commentary variability among other relevant fungi, how molecular aforementioned still strong base dissecting responses such species.

Язык: Английский

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Nitric oxide synthesis by nitrate reductase is regulated during development in Aspergillus

Molecular Microbiology, Год журнала: 2015, Номер 99(1), С. 15 - 33

Опубликована: Сен. 10, 2015

Summary Nitric oxide ( NO ) is a signalling molecule involved in many biological processes bacteria, plants and mammals. However, little known about the role biosynthesis of fungi. Here we show that production increased at early stages transition from vegetative growth to development A spergillus nidulans . Full requires functional nitrate reductase NR gene nia D upregulated upon induction conidiation, even under N ‐repressing conditions presence ammonium. At this stage, homeostasis achieved by balancing catabolism (flavohaemoglobins). flavohaemoglobin fhb are transiently both regulators AreA NirA necessary for transcriptional response. The second B shows different expression profile being moderately expressed during phase but it strongly induced 24 h later. levels influence balance between conidiation sexual reproduction because artificial strong elevation reduced formation cleistothecia. nitrate‐independent nitrogen metabolite repression‐insensitive upregulation suggests novel linking metabolism development.

Язык: Английский

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