Schizosaccharomyces lindneri sp. nov., a fission yeast occurring in honey

Yeast, Год журнала: 2023, Номер 40(7), С. 237 - 253

Опубликована: Май 27, 2023

Abstract Two strains of fission yeast were isolated from honey. They differ the type strain Schizosaccharomyces octosporus by three substitutions in D1/D2 domain nuclear 26S large subunit ribosomal RNA (rRNA) gene sequence, resulting a 99.5% identity. In internal transcribed spacer (ITS) region (consisting ITS1, 5.8S rDNA, and ITS2), S. 16 gaps 91 substitutions, which is equivalent to an identity 88.1%. Genome sequencing on one new revealed that average nucleotide (ANI) between its genome reference 90.43% there exist major rearrangements two genomes. Mating analysis are completely reproductively separated. A strong prezygotic barrier exists few mating products consist diploid hybrids do not form recombinant ascospores. strains, asci either zygotic, arising conjugation, or they develop without conjugation asexual cells (azygotic). Compared currently recognized species, spectrum nutrients assimilated restricted. Of 43 carbohydrates included physiological standard tests, only 7 assimilated. According results sequence analysis, trials, phenotypic characterization, species lindneri described accommodate (holotype: CBS 18203 T ex‐type: MUCL 58363; MycoBank no.: MB 847838).

Язык: Английский

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Fast label-free microscopy technique for 3D dynamic quantitative imaging of living cells

Biomedical Optics Express, Год журнала: 2017, Номер 8(12), С. 5507 - 5507

Опубликована: Ноя. 9, 2017

The refractive index (RI) is an important optical characteristic that often exploited in label-free microscopy for analysis of biological objects.A technique 3D RI reconstruction living cells has to be fast enough capture the cell dynamics and preferably needs compatible with standard wide-field microscopes.To solve this challenging problem, we present a provides measurement processing data required real-time visualization object RI.Specifically, reconstructed from bright-field intensity images, axially scanned by high-speed focus tunable lens mounted front sCMOS camera, using direct deconvolution approach designed partially coherent light non-paraxial regime.Both system illumination, sectioning speckle-noise suppression, enable compatibility microscopes resulting competitive affordable alternative current holographic laser microscopes.Our experimental demonstrations show video-rate bacteria both freely swimming optically manipulated freestyle traps allowing their trapping transport along trajectories.These results prove possible conduct simultaneous 4D quantitative imaging manipulation cells, which promising study biophysics biology.

Язык: Английский

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A Brief History ofSchizosaccharomyces pombeResearch: A Perspective Over the Past 70 Years

Genetics, Год журнала: 2016, Номер 203(2), С. 621 - 629

Опубликована: Июнь 1, 2016

Abstract Since its humble start as a model organism in two European laboratories the 1940s and 1950s, fission yeast Schizosaccharomyces pombe has grown to become one of best-studied eukaryotes today. This article outlines way which interest S. developed spread from Europe Japan, North America, elsewhere beginnings up first International Meeting devoted this 1999. We describe expansion research during period with an emphasis on many individual researchers involved their interactions that resulted development today’s vibrant community.

Язык: Английский

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Zinc sensing and regulation in yeast model systems

Archives of Biochemistry and Biophysics, Год журнала: 2016, Номер 611, С. 30 - 36

Опубликована: Март 3, 2016

Язык: Английский

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3D patterning/manipulating microparticles and yeast cells using ZnO/Si thin film surface acoustic waves

Sensors and Actuators B Chemical, Год журнала: 2019, Номер 299, С. 126991 - 126991

Опубликована: Авг. 17, 2019

Язык: Английский

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