Arranged
in
a
spatial-temporal
gradient
for
germ
cell
development,
the
adult
germline
of
Caenorhabditis
elegans
is
an
excellent
system
understanding
generation,
differentiation,
function,
and
maintenance
cells.
Imaging
whole
C.
germlines
along
distal-proximal
axis
enables
powerful
cytological
analyses
nuclei
as
they
progress
from
pre-meiotic
tip
through
all
stages
meiotic
prophase
I.
To
enable
high-content
image
analysis
gonads,
we
developed
custom
algorithm
pipelines
to
function
with
processing
software
that
enables:
(1)
quantification
features
at
single
nucleus
resolution
immunofluorescence
images;
(2)
assessment
these
individual
based
on
their
position
within
germline.
We
show
capability
our
quantitative
approach
by
analyzing
multiple
germlines.
First,
quantify
double-strand
DNA
breaks
(DSBs)
per
DNA-associated
foci
recombinase
RAD-51
single-nucleus
context
progression.
Second,
DSBs
are
licensed
crossover
repair
MSH-5
COSA-1
when
associate
synaptonemal
complex
during
Finally,
P-granule
composition
across
colocalization
PGL-1
ZNFX-1
foci.
Our
pipeline
adaptable
useful
method
researchers
spanning
fields
using
model
system.
Abstract
Life
history
is
defined
by
traits
that
reflect
key
components
of
fitness,
especially
those
relating
to
reproduction
and
survival.
Research
in
life
seeks
unravel
the
relationships
among
these
understand
how
strategies
evolve
maximize
fitness.
As
such,
research
integrates
study
genetic
developmental
mechanisms
underlying
trait
determination
with
evolutionary
ecological
context
Darwinian
a
leading
model
organism
for
molecular
genetics,
Caenorhabditis
elegans
unmatched
characterization
history-related
processes,
including
timing
plasticity,
reproductive
behaviors,
sex
determination,
stress
tolerance,
aging.
Building
on
recent
studies
natural
populations
ecology,
combination
C.
elegans’
historical
strengths
new
insights
into
variation
now
positions
it
as
uniquely
valuable
research.
In
this
review,
we
summarize
contributions
related
species
its
evolution.
We
begin
reviewing
characteristics
history,
an
emphasis
distinctive
notable
cycle
plasticity.
Next,
explore
intraspecific
architecture.
Finally,
provide
overview
has
guided
major
transitions
both
within
genus
across
broader
phylum
Nematoda.
While
relatively
research,
significant
progress
been
made
leveraging
biological
traits,
establishing
highly
cross-disciplinary
system
studies.
Genetics,
Год журнала:
2020,
Номер
214(4), С. 869 - 893
Опубликована: Фев. 15, 2020
Mutations
affecting
spliceosomal
proteins
are
frequently
found
in
hematological
malignancies,
including
myelodysplastic
syndromes
and
acute
myeloid
leukemia
(AML).
DDX41/Abstrakt
is
a
metazoan-specific
DEAD-box
RNA
helicase
that
recurrently
mutated
inherited
relapsing
cases
of
AML.
The
genetic
properties
genomic
impacts
disease-causing
missense
mutations
DDX41
other
have
been
uncertain.
Here,
we
conduct
comprehensive
analysis
the
Caenorhabditis
elegans
ortholog,
SACY-1
Biochemical
analyses
defined
as
component
C.
spliceosome,
revealed
synthetic
lethal
interactions
with
components.
We
used
auxin-inducible
degradation
system
to
analyze
consequence
depletion
on
transcriptome
using
sequencing.
through
splicing-dependent
splicing-independent
mechanisms.
Altered
3'
splice
site
usage
represents
predominant
splicing
defect
observed
upon
depletion,
consistent
role
for
second
step
splicing.
Missplicing
events
appear
more
prevalent
soma
than
germline,
suggesting
surveillance
mechanisms
protect
germline
from
aberrant
changes
after
suggest
disruption
spliceosome
induces
stress
response,
which
could
contribute
cellular
phenotypes
conferred
by
sacy-1
mutant
alleles.
Multiple
sacy-1/ddx41
mutations,
R525H
human
oncogenic
variant,
confer
antimorphic
activity,
their
incorporation
into
detrimental.
Antagonistic
variants
perturb
function
may
be
relevant
DDX41,
highly
conserved
components
humans.
Nature Communications,
Год журнала:
2021,
Номер
12(1)
Опубликована: Ноя. 18, 2021
Abstract
Communication
between
the
soma
and
germline
optimizes
germ
cell
fate
programs.
Notch
receptors
are
key
determinants
of
but
how
somatic
signals
direct
Notch-dependent
behavior
is
undefined.
Here
we
demonstrate
that
SDN-1
(syndecan-1),
a
transmembrane
proteoglycan,
controls
expression
GLP-1
(germline
proliferation-1)
receptor
in
Caenorhabditis
elegans
germline.
We
find
control
TRP
calcium
channel
governs
calcium-dependent
binding
an
AP-2
transcription
factor
(APTF-2)
to
glp-1
promoter.
Hence,
signaling
promotes
mitotic
fate.
Together,
these
data
reveal
as
putative
communication
nexus
its
environment
decisions.
Arranged
in
a
spatial-temporal
gradient
for
germ
cell
development,
the
adult
germline
of
Caenorhabditis
elegans
is
an
excellent
system
understanding
generation,
differentiation,
function,
and
maintenance
cells.
Imaging
whole
C.
germlines
along
distal-proximal
axis
enables
powerful
cytological
analyses
nuclei
as
they
progress
from
pre-meiotic
tip
through
all
stages
meiotic
prophase
I.
To
enable
high-content
image
analysis
gonads,
we
developed
custom
algorithm
pipelines
to
function
with
processing
software
that
enables:
(1)
quantification
features
at
single
nucleus
resolution
immunofluorescence
images;
(2)
assessment
these
individual
based
on
their
position
within
germline.
We
show
capability
our
quantitative
approach
by
analyzing
multiple
germlines.
First,
quantify
double-strand
DNA
breaks
(DSBs)
per
DNA-associated
foci
recombinase
RAD-51
single-nucleus
context
progression.
Second,
DSBs
are
licensed
crossover
repair
MSH-5
COSA-1
when
associate
synaptonemal
complex
during
Finally,
P-granule
composition
across
colocalization
PGL-1
ZNFX-1
foci.
Our
pipeline
adaptable
useful
method
researchers
spanning
fields
using
model
system.
