Enzymatic Modification of Apple Pomace and Its Application in Conjunction with Probiotics for Jelly Candy Production
Applied Sciences,
Год журнала:
2025,
Номер
15(2), С. 599 - 599
Опубликована: Янв. 9, 2025
This
study
aimed
to
evaluate
the
applicational
possibilities
of
enzymatically
modified
apple
pomace
(AP)
in
conjunction
with
probiotics
as
value-added
ingredients
for
production
jelly
candies.
AP
was
Pectinex®
Ultra
Tropical,
Viscozyme®
L,
and
Celluclast®
1.5
L
(Novozyme
A/S,
Bagsværd,
Denmark),
soluble
insoluble
dietary
fibre
content
determined
using
Megazyme
kit
(Megazyme
International
Ireland
Ltd.,
Wicklow,
Ireland),
reducing
sugar
3,5-dinitrosalicylic
acid
assay.
The
technological
properties
AP,
such
its
swelling
capacity,
water-retention
oil-retention
bulk
density,
static
thermal
emulsion
stability,
were
evaluated.
Enzymatically
hydrolysed
used
candies
supplemented
Bifidobacterium
animalis
DSM
20105.
survival
during
vitro
digestion,
viability
candy
storage,
quality
characteristics
analysed.
had
different
carbohydrate
compositions
properties,
depending
on
enzyme
preparation
used.
Although
decreased
a
significantly
higher
B.
compared
control
made
without
after
digestion
saline,
gastric,
intestine
phases.
shows
that
can
be
increasing
(18.4%),
which
further
applied,
animalis,
added-value
production.
Язык: Английский
Advances in understanding therapeutic mechanisms of probiotics in cancer management, with special emphasis on breast cancer: a comprehensive review
European Journal of Pharmacology,
Год журнала:
2025,
Номер
unknown, С. 177410 - 177410
Опубликована: Фев. 1, 2025
Язык: Английский
SUPPRESSION OF PROLIFERATION AND INCREASED OF PRO-APOPTOTIC PROTEINS EXPRESSION IN HUMAN BREAST CANCER CELLS AFTER THEIR CO-CULTIVATION WITH BIFIDOBACTERIUM ANIMALIS IN VITRO
Oncology,
Год журнала:
2024,
Номер
25(1), С. 29 - 37
Опубликована: Янв. 1, 2024
Summary.
Aim:
to
determine
the
viability,
proliferative
activity,
and
reactive
oxygen
species
(ROS)
production
in
human
breast
cancer
(BC)
cells
after
their
co-cultivation
with
a
microbiota
member
Bifidobacterium
animalis.
Object
methods:
study
was
conducted
vitro
on
BC
of
MCF7,
T47D,
MDA-MB-231
lines.
Cells
were
co-cultured
live
B.
animalis
bacteria
direct
or
metabolite-mediated
(contactless
Insert
system)
interaction
between
eukaryotic
bacterial
cells.
ROS
assessed
by
flow
cytometry.
The
expression
proteins
associated
regulation
proliferation
apoptosis
evaluated
immunocytochemical
analysis.
Results:
most
pronounced
changes
growth
characteristics
are
caused
malignant
cells,
compared
action.
MCF-7
luminal
subtype
sensitive
effects
Co-cultivation
leads
decrease
Ki-67
an
increase
production,
pro-apoptotic
Bax
p21WAF1.
Translocalization
p21WAF1
from
cytoplasm
cell
nucleus
detected
T47D
exposure
Conclusions:
secreted
metabolites
led
inhibition
proliferation,
induction
oxidative
stress,
program
activation
Язык: Английский