Metabolomic Comparison of Guava (Psidium guajava L.) Leaf Extracts Fermented by Limosilactobacillus fermentum and Lactiplantibacillus plantarum and Their Antioxidant and Antiglycation Activities

Nutrients, Год журнала: 2024, Номер 16(6), С. 841 - 841

Опубликована: Март 14, 2024

Probiotic fermentation of plant-based materials can lead to the generation various bioactive substances via bacterial metabolites and biotransformation phenolic compounds. We compared metabolic differences between by Limosilactobacillus fermentum KCTC15072BP (LFG) Lactiplantibacillus plantarum KGMB00831 (LPG) in guava leaf extract (0%, 0.5%, 2% (w/v))-supplemented medium non-targeted metabolite profiling. By performing multivariate statistical analysis comparing different groups, 21 guava-derived 30 were identified. The contents glucogallin, gallic acid, sugar alcohols significantly higher LFG than they LPG. Similarly, pyrogallol, vanillic naringenin, phloretin, aromatic amino acid catabolites obtained with LPG LFG. led antioxidant activities LPG, while antiglycation activity Interestingly, fermentation-induced increase guava-leaf-extract-supplemented group was that control group. Thus, increased bioactivity induced Lactobacillaceae strain may be influenced synergistic effects microbial plant-derived Overall, examining changes food differentiating origin provides a better understanding fermentation.

Язык: Английский

---

Precision medicine for personalized cholecystitis care: integrating molecular diagnostics and biotherapeutics

Bulletin of the National Research Centre/Bulletin of the National Research Center, Год журнала: 2024, Номер 48(1)

Опубликована: Сен. 12, 2024

Abstract Background Acute cholecystitis, inflammation of the gallbladder, can lead to serious complications if not promptly diagnosed and managed. Conventional therapies have limitations, necessitating newer personalized approaches. Main body abstract This review examines recent advances transforming cholecystitis care. Diagnostically, molecular techniques like next-generation sequencing rapidly identify causative microbes from gallbladder specimens, enabling targeted antimicrobial therapy. Regarding treatment, phage therapy uses viruses lyse pathogenic bacteria. RNA interference CRISPR-Cas9 gene editing silence microbial virulence factors. Probiotics competitively exclude pathogens. Robotics fluorescence imaging refine surgical techniques. Additional emerging modalities include biosensors detecting inflammatory mediators, regenerative tissue engineering using stem cells, artificial intelligence for real-time decision support. However, optimal integration novel technologies with current best practices remains unknown. Further research is needed validate optimize diagnostics therapeutics cholecystitis. Short conclusions Advances in sequencing, CRISPR editing, robotics, other biotechnologies promise transform precision management when thoughtfully implemented. controlled trials are still required define conventional supportive care antibiotics.

Язык: Английский

---

An insight into probiotic microorganisms

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 17 - 50

Опубликована: Янв. 1, 2025

Язык: Английский

---

Integrating Metabolomics and Genomics to Uncover Antimicrobial Compounds in Lactiplantibacillus plantarum UTNGt2, a Cacao-Originating Probiotic from Ecuador

Antibiotics, Год журнала: 2025, Номер 14(2), С. 123 - 123

Опубликована: Янв. 24, 2025

Background/Objectives: Lactic acid bacteria (LAB) produce several diverse metabolites during fermentation that play key roles in enhancing health and food quality. These include peptides, organic acids, exopolysaccharides, antimicrobial compounds, which contribute to gut health, immune system modulation, pathogen inhibition. This study analyzed the intracellular (Met-Int) extracellular (Met-Ext-CFS; cell-free supernatant) of Lactiplantibacillus plantarum UTNGt2, a probiotic strain isolated from Theobroma grandiflorum. Methods: The assessment was performed using capillary LC-MS/MS metabolomics with SWATH-based data-independent acquisition approach identify molecules associated activity. Results: integration metabolomic data whole-genome annotation enabled identification metabolites, including amino nucleotides, oligopeptides, terpenes, flavonoids, many were activity UTNGt2. Pathway analysis reveals critical processes such as secondary metabolite biosynthesis, nucleotide galactose metabolism, cofactor biosynthesis. By integrating RiPP (ribosomally synthesized post-translationally modified peptide) cluster gene predictions LC-MS data, this validates production specific RiPPs uncovers novel bioactive compounds encoded within UTNGt2 genome. oligopeptide val-leu-pro-val-pro-gln found both Met-Int (ESI+) Met-Ext-CFS may strain’s strength. It could also enhance fermentation-related functions. Conclusions: While genome-based highlight biosynthetic potential, actual profile is influenced by factors like transcriptional regulation, post-transcriptional post-translational modifications, environmental conditions. findings emphasize value multi-omics approaches providing holistic understanding its role

Язык: Английский

---

Biogenic-Synthesized Silver Nanoparticles Using the Ligilactobacillus salivarius KC27L Postbiotic: Antimicrobial, Anti-Biofilm, and Antioxidant Activity and Cytotoxic Effects

Probiotics and Antimicrobial Proteins, Год журнала: 2025, Номер unknown

Опубликована: Фев. 26, 2025

Abstract This study aimed to synthesize silver nanoparticles (AgNPs) using the postbiotic of Ligilactobacillus salivarius KC27L strain and evaluate their multifunctional biological properties. The use L. , a probiotic bacterium known for its ability produce wide range metabolites, plays crucial role in this process by acting as natural, eco-friendly reducing, stabilizing agent during AgNP synthesis. approach not only eliminates need hazardous chemicals typically used nanoparticle synthesis but also enhances biocompatibility efficacy resulting nanoparticles. Synthesized AgNPs were analyzed Fourier transform infrared spectroscopy, FTIR (metabolites postbiotic); UV–vis (peak 435 nm); scanning electron microscope, SEM; transmission microscopy, TEM (spherical shapes, sizes < 50 nm), energy-dispersive spectrometry, EDS at 3 keV); zeta potential (− 18.6 mV). These (0.156–40 mg/mL) evaluated antimicrobial anti-biofilm activities against Escherichia coli ATCC 11229, Pseudomonas aeruginosa 27853, Staphylococcus aureus 25923, epidermidis 35984, Streptococcus mutans 25175, antioxidant four different methods (2,2-diphenyl-1-picrylhydrazyl free radical scavenging, metal ion chelating, hydroxyl superoxide anion scavenging activities). Also, cytotoxic activity was investigated normal cell line (L929) 24, 48, 72 h. At concentration 40 mg/mL, demonstrated highest efficacy, with inhibition zones measured 14.9 mm P. 9.5 E. 15.7 S. 12.9 . exhibited all Gram-positive Gram-negative bacteria strains studied. According DPPH method, determined mg/mL (80.93%). found have no toxic effect low concentrations (0.39–25 µg/mL). Biogenic synthesized could be biotechnological applications (biomaterials, health, environmental, etc.) antibacterial, anti-biofilm, antioxidant, nontoxic However, further research is needed understand mechanisms action particles fully.

Язык: Английский

---

Behaviors of useful bacterial communities associated with the cultivation of Aurantiochytrium sp. strain L3W using fermented wastes at different pH values

Journal of Material Cycles and Waste Management, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

---

Bioactive Compounds and Antioxidant Properties of Sheep’s Milk Yogurt Enriched with a Postbiotic Extract from Lactiplantibacillus plantarum UCLM56: Effects of In Vitro Digestion and Fermentation

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 14, 2025

Lactic acid bacteria present in yogurts produce bioactive compounds, such as gamma-aminobutyric (GABA) and short-chain fatty acids (SCFAs). Sheep's milk yogurt is an excellent medium to enhance their properties. In this study, the impact of adding extract derived from GABA-producing strain Lactiplantibacillus plantarum UCLM56 sheep's was evaluated terms its antioxidant Samples were subjected vitro digestion fermentation simulate effects gastrointestinal tract. GABA, SCFAs, amino acids, biogenic amines, capacity, organic levels analyzed. Yogurt enriched with postbiotic showed higher GABA propionic acid, increases more than 360 260%, respectively, along improved capacity (FRAP DPPH methods) compared conventional yogurt. After fermentation, lactic significantly (50 41% increases, respectively), well (more 200% improvement). conclusion, use enhances properties yogurt, making it a promising option for developing functional dairy products added value.

Язык: Английский

---

Deciphering the Effect of Millet Type on the Structure of the Bacterial Community in an Indian Fermented Beverage

Food Safety and Health, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

ABSTRACT Millets have garnered widespread popularity due to their nutritional value and gluten‐free nature. Many studies shown the promising effect of fermentation on millets. Despite this, potential in fermented food products remains largely underexplored. Traditionally, millets were widely used prepare naturally beverages. However, not much is known about microbial profiles these Therefore, for very first time, we aimed conduct a comparative investigation composition millet‐based beverage. The beverage was prepared using five different millet varieties, namely, pearl (PM), kodo (KM), barnyard (BM), little (LM), finger (FM). processing beverages inspired by traditional Indian koozh. 16S amplicon sequencing determine bacterial community results indicated dominance Firmicutes Proteobacteria at phylum level all beverages; however, ratios varied depending substrates. genus Weissella appears be most dominant samples, with exception LM‐based differences, PICRUSt functional analysis stable core characteristics This study laid foundational work further explore as substrate, particularly growth lactic acid bacteria.

Язык: Английский

---

Microbial diversity and fitness in the gut–brain axis: influences on developmental risk for Alzheimer’s disease

Gut Microbes, Год журнала: 2025, Номер 17(1)

Опубликована: Апрель 10, 2025

The gut-brain axis (GBA) denotes the dynamic and bidirectional communication system that connects gastrointestinal tract central nervous (CNS). This review explored this axis, focusing on role of microbial diversity fitness in maintaining health preventing neurodegeneration, particularly Alzheimer's disease (AD). Gut dysbiosis, characterized by imbalance populations beneficial harmful bacteria, has been associated with increased systemic inflammation, neuroinflammation, progression AD through pathogenic mechanisms involving amyloid deposition, tauopathy, blood-brain barrier (BBB) permeability. Emerging evidence highlighted therapeutic potential probiotics, dietary interventions, intermittent fasting restoring balance, reducing minimizing neurodegenerative risks. Probiotics synbiotics are promising helping improve cognitive function metabolic health, while patterns like Mediterranean diet were linked to decreased neuroinflammation enhanced communication. Despite significant advancement, further research is needed elucidate specific strains, metabolites, influencing brain health. Future studies employing longitudinal designs advanced omics technologies essential developing targeted microbiome-based therapies for managing AD-related disorders.

Язык: Английский

---

Advancing gut microbiome insights: Graphene quantum dot nanobiosensors for microbial metabolite detection

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 162954 - 162954

Опубликована: Апрель 1, 2025

---