Raman spectral analysis of the brainstem and responses of neuroglia and cytokines in whole-body gamma-irradiated rats after administration of aminothiol-based radioprotector GL2011 DOI Creative Commons

Dušica M Kočović,

Danica Bajuk‐Bogdanović, Irina Maslovarić

и другие.

Archives of Biological Sciences, Год журнала: 2021, Номер 73(2), С. 161 - 173

Опубликована: Янв. 1, 2021

The search for an effective and non-toxic radioprotector is ongoing. We tested a novel, natural aminothiol-based radioprotector, GL2011, that was applied 30 min, 3 h or 6 after the exposure of male albino Wistar rats to 6.7 Gy mild dose gamma radiation. molecular signatures radioprotection were investigated with Raman microspectroscopy brainstem tissue samples. Morphological changes activation astrocytes microglia assessed by immunohistochemistry. Global markers neuroinflammation followed ELISA monitor blood plasma levels proinflammatory (IL-6 TNF-?) anti-inflammatory (IL-10) cytokines. A thirty-day follow-up determined survival unprotected animals 37.5%. increase observed (75%, irrespective time application). spectra revealed slightly deleterious effect radiation on nucleic acids in surviving mitigated as GL2011 preserved morphology both microglia, reduced microglial infiltration. Cytokine assessment immunomodulatory novel radioprotector. overall results point out positive effects single at different times. cellular indicate conferred better protection, which underlines its translation cure accidents.

Язык: Английский

Pre-clinical Research on Bladder Toxicity After Radiotherapy for Pelvic Cancers: State-of-the Art and Challenges DOI Creative Commons
Stefania Zuppone, A. Bresolin, Antonello E. Spinelli

и другие.

Frontiers in Oncology, Год журнала: 2020, Номер 10

Опубликована: Окт. 22, 2020

Despite the dramatic advancements in pelvic radiotherapy, urinary toxicity remains a significant side-effect. The assessment of clinico-dosimetric predictors radiation cystitis (RC) based on clinical data has improved substantially over last decade; however, thorough understanding physiopathogenetic mechanisms underlying onset RC, with its variegated acute and late symptoms, is still largely lacking, from pre-clinical research limited. aim this review to provide an overview main open issues and, ideally, help investigators orienting future research. First, anatomy physiology bladder, as well current knowledge dose dose-volume effects humans, are briefly summarized. Subsequently, radiobiology aspects RC discussed. findings suggest that animal models lively field growing interest development new radioprotective agents. availability high precision micro-irradiators rapid advances small imaging might lead big improvement into field. In particular, studies focusing definition fractionation warranted, especially considering hypo-fractionation ablative therapies for prostate cancer treatment. Moreover, radiotherapy plans optimization by selectively reducing more radiosensitive substructures close bladder would be paramount importance. Finally, thanks platforms, reliable reproducible methods assess severity expected developed.

Язык: Английский

Процитировано

12
Pharmacologic Protection in Distant Space: Current View DOI
И. Б. Ушаков,

M. V. Vasin

Biology Bulletin, Год журнала: 2019, Номер 46(11), С. 1524 - 1532

Опубликована: Дек. 1, 2019

Язык: Английский

Процитировано

4
Looking for the phoenix: the current research on radiation countermeasures DOI Creative Commons
Vojtěch Chmil, Alžběta Filipová, Aleš Tichý

и другие.

International Journal of Radiation Biology, Год журнала: 2023, Номер 99(8), С. 1148 - 1166

Опубликована: Фев. 6, 2023

Purpose Ionizing radiation (IR) is widely applied in radiotherapy for the treatment of over 50% cancer patients. IR also intensively used medical diagnostics on a daily basis imaging. Moreover, recent geopolitical events have re-ignited real threat use nuclear weapons. Medical countermeasures represent one effective protection strategies against effects IR. The aim this review was to summarize most commonly and procedures development evaluate current state their research, with focus those clinical trial phase.Methods Clinical trials were selected accordance preferred reporting items systematic reviews meta-analyses (PRISMA) statement. search performed clinicaltrials.gov database as May 2022.Results Our returned 263 studies, which screened 25 included review. 10 these studies had been completed, 3 promising results: KMRC011 increased G-CSF, IL-6, neutrophil counts suggesting potential hematopoietic acute syndrome (H-ARS); GC4419 reduced number patients severe oral mucositis its duration; combination enoxaparin, pentoxifylline, ursodeoxycholic acid incidence focal radiation-induced liver injury.Conclusion agents discovered so far show significant side or low efficacy, hence tested terminate early stages development. In addition, profitability type drug demotivates private sector invest such research. To overcome problem, there need involve more public resources funding. Among technological opportunities, deeper silico approaches seems be prospective.

Язык: Английский

Процитировано

1
Mitigation of Iron Irradiation-Induced Genotoxicity and Genomic Instability by Postexposure Dietary Restriction in Mice DOI Creative Commons
Bing Wang, Takanori Katsube, Kaoru Tanaka

и другие.

BioMed Research International, Год журнала: 2021, Номер 2021, С. 1 - 12

Опубликована: Ноя. 25, 2021

Background and Purpose. Postexposure onset of dietary restriction (DR) is expected to provide therapeutic nutritional approaches reduce health risk from exposure ionizing radiation (IR) due such as manned space exploration, radiotherapy, or nuclear accidents IR could alleviate radiocarcinogenesis in animal models. However, the underlying mechanisms remain largely unknown. This study aimed at investigating effect postexposure DR on genotoxicity genomic instability (GI) induced by total body irradiation (TBI) mice. Materials Methods. Mice were exposed 2.0 Gy accelerated iron particles with an initial energy 500 MeV/nucleon a linear transfer (LET) value about 200 keV/μm. After TBI, mice either allowed free access standard laboratory chow treated under (25% cut diet). Using micronucleus frequency (MNF) bone marrow erythrocytes, induction acute GI hematopoietic system was, respectively, determined 1 2 months after TBI. Results Conclusions. TBI alone caused significant increase MNF while did not markedly influence MNF. decrease compared treatment alone. demonstrated that relieve elevated high-LET particles. These findings indicated reduction late may be least part decreased DR.

Язык: Английский

Процитировано

2
B-190 (Indralin) in Light of the History of the Formation of Ideas about the Mechanism of Action of Radioprotectors DOI

M. V. Vasin

Biology Bulletin, Год журнала: 2021, Номер 48(11), С. 2045 - 2059

Опубликована: Дек. 1, 2021

Язык: Английский

Процитировано

2
More efficient induction of genotoxicity by high-LET Fe-particle radiation than low-LET X-ray radiation at low doses DOI Creative Commons
Bing Wang, Takanori Katsube, Kaoru Tanaka

и другие.

Radiation Medicine and Protection, Год журнала: 2022, Номер 4(1), С. 11 - 18

Опубликована: Дек. 30, 2022

To understand differential effects on induction of genotoxicity and genomic instability (GI) by high-LET particle radiation low-LET photon radiation, based ground-based experiments using total body irradiation (TBI) mice with Fe-particle X-ray radiation. TBI was delivered to C57BL/6J Jms strain female 8 weeks old at a dose ranging from 0.1 3.0 Gy or 5.0 Induction GI determined respectively 1 2 months after exposure frequency micronuclei in bone marrow erythrocytes as the endpoint. Inhibition cell proliferation measured reduced erythropoiesis. Physiological conditions were also investigated. TBI, regardless type caused statistically significant increase month exposure, but did not induce even higher doses (>1.0 Gy). The dose-response curve for micronucleated polychromatic induced y ​= ​0.7798 ​+ ​1.7889x– 0.5978x2 (R2 = 0.8109) ​0.7421 ​1.3792x – 0.2588 x2 0.8081), respectively. normochromatic ​0.7191 ​1.4545x 0.4978x2 0.7047) ​0.658 ​1.344x 0.2531x2 0.7853), In general, more efficient inducing than lower (<0.5 These results further confirm that does increased erythrocytes. findings indicate radiation-induced is mouse dependent suggest comprehensive studies should be done explore late health consequences low doses.

Язык: Английский

Процитировано

1
Raman spectral analysis of the brainstem and responses of neuroglia and cytokines in whole-body gamma-irradiated rats after administration of aminothiol-based radioprotector GL2011 DOI Creative Commons

Dušica M Kočović,

Danica Bajuk‐Bogdanović, Irina Maslovarić

и другие.

Archives of Biological Sciences, Год журнала: 2021, Номер 73(2), С. 161 - 173

Опубликована: Янв. 1, 2021

The search for an effective and non-toxic radioprotector is ongoing. We tested a novel, natural aminothiol-based radioprotector, GL2011, that was applied 30 min, 3 h or 6 after the exposure of male albino Wistar rats to 6.7 Gy mild dose gamma radiation. molecular signatures radioprotection were investigated with Raman microspectroscopy brainstem tissue samples. Morphological changes activation astrocytes microglia assessed by immunohistochemistry. Global markers neuroinflammation followed ELISA monitor blood plasma levels proinflammatory (IL-6 TNF-?) anti-inflammatory (IL-10) cytokines. A thirty-day follow-up determined survival unprotected animals 37.5%. increase observed (75%, irrespective time application). spectra revealed slightly deleterious effect radiation on nucleic acids in surviving mitigated as GL2011 preserved morphology both microglia, reduced microglial infiltration. Cytokine assessment immunomodulatory novel radioprotector. overall results point out positive effects single at different times. cellular indicate conferred better protection, which underlines its translation cure accidents.

Язык: Английский

Процитировано

1