Molecular Accounting and Profiling of Human Respiratory Microbial Communities: Toward Precision Medicine by Targeting the Respiratory Microbiome for Disease Diagnosis and Treatment

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(4), С. 4086 - 4086

Опубликована: Фев. 17, 2023

The wide diversity of microbiota at the genera and species levels across sites individuals is related to various causes observed differences between individuals. Efforts are underway further understand characterize human-associated its microbiome. Using 16S rDNA as a genetic marker for bacterial identification improved detection profiling qualitative quantitative changes within population. In this light, review provides comprehensive overview basic concepts clinical applications respiratory microbiome, alongside an in-depth explanation molecular targets potential relationship microbiome disease pathogenesis. paucity robust evidence supporting correlation pathogenesis currently main challenge not considering novel druggable target therapeutic intervention. Therefore, studies needed, especially prospective studies, identify other drivers better in lung along with association medications. Thus, finding unfolding significance would be crucial.

Язык: Английский

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Asthma-COPD Overlap in Clinical Practice (ACO_CP 2023): Toward Precision Medicine

Journal of Personalized Medicine, Год журнала: 2023, Номер 13(4), С. 677 - 677

Опубликована: Апрель 18, 2023

Asthma and COPD have characteristic symptoms, yet patients with both are prevalent. Despite this, there is currently no globally accepted definition for the overlap between asthma COPD, commonly referred to as asthma–COPD (ACO). Generally, ACO not considered a distinct disease or symptom from either clinical mechanistic perspectives. However, identifying who present conditions crucial guiding therapy. Similar heterogeneous presumably multiple underlying processes. The variability of led establishment definitions describing condition’s essential clinical, physiological, molecular characteristics. comprises numerous phenotypes, which affects optimal medication choice can serve predictor prognosis. Various phenotypes been suggested based on host factors including but limited demographics, spirometric findings, smoking history, airway inflammation. This review provides comprehensive guide be used in practice available data. Future longitudinal studies must evaluate stability over time explore their predictive powers facilitate more precise effective management approach.

Язык: Английский

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Alteration of the Respiratory Microbiome in Hospitalized Patients with Asthma–COPD Overlap during and after an Exacerbation

Journal of Clinical Medicine, Год журнала: 2023, Номер 12(6), С. 2118 - 2118

Опубликована: Март 8, 2023

The immediate aim of this study was to comparatively examine the bacterial respiratory microbiome patients in a stable state and during an exacerbation asthma–COPD (chronic obstructive pulmonary disease) overlap (ACO). This prospective observational took place Jordan between 1 September 2021 30 April 2022. Sputum samples from with recognized ACO were acquired within 48 h onset again at 3 weeks following exacerbation. next-generation sequencing Illumina MiSeq employed uncovered significantly high diversity sputa. results showed significant decrease taxonomic richness sputum collected episodes compared those (p = 0.008), increase evenness < 0.005). change composition airway community suggests that replacement portion certain microorganisms may play role microbial observed Greater knowledge link could allow for more focused administration antibiotics, especially exacerbations, improving clinical efficacy patient outcomes.

Язык: Английский

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The role of human rhinovirus in COPD exacerbations in Abu Dhabi: molecular epidemiology and clinical significance

Libyan Journal of Medicine, Год журнала: 2024, Номер 19(1)

Опубликована: Янв. 30, 2024

This study aimed to describe the molecular epidemiology and seasonality of human rhinovirus (HRV) in chronic obstructive pulmonary disease (COPD) its association with COPD exacerbations Abu Dhabi, United Arab Emirates (UAE). Sputum specimens were collected for analysis from all patients who visited a medical center November 2021 October 2022. The real-time quantitative polymerase chain reaction (qPCR) test was used detect HRV. Of 78 included study, 58 (74%) presented one or more exacerbation episodes. incidence peaked over winter substantially decreased during summer. HRV positivity (E1) 11/58 (19%) 15/58 (26%) two weeks after episode (E2). There no significant difference load these patients. No statistically observed detection compared stable COPD. is first assess between by qPCR UAE. high sensitivity technology helped collect reliable epidemiologic data. Few studies have provided similar Middle East study’s pattern parallels that temperate countries. information can help future, extensive surveillance respiratory viruses UAE their exacerbations.

Язык: Английский

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Assessing the Pharmacist’s Role in Counseling Asthmatic Adults Using the Correct Inhaler Technique and Its Effect on Asthma Control, Adherence, and Quality of Life

Patient Preference and Adherence, Год журнала: 2023, Номер Volume 17, С. 961 - 972

Опубликована: Апрель 1, 2023

Purpose: This study was designed to assess inhaler technique skills, asthma control level, adherence, and quality of life scores amongst asthmatic patients living in Amman, Jordan, investigating the role pharmacist's educational intervention. Patients Methods: single-blinded randomized parallel-group active-controlled conducted at Jordan University Hospital (JUH) clinic 2019. were assessed baseline follow-up (after three months) on their (based validated published checklists), based test (ACT) questionnaire, adherence treatment, (QoL). active group counseled correct administration orally using adhesive labels delivered by clinical pharmacist. Results: Asthma (n = 157; active, n 79, control, 78) recruited into grouped controller they (Turbohaler (TH) Accuhaler (ACC). There no statistically significant difference between both groups for technique, ACT, QoL. At follow-up, 93.7% used inhalers correctly, while only 16.7% them correctly (P-value < 0.001). Also, ACT mean score significantly higher (21.21± 5.27) versus (15.92± 7.31; P-value The QoL improved compared with (active 63.85± 24.78, Control 38.746± 3.23, Improvement noticed but did not reach a level (P-value=0.691). Conclusion: Through practical intervention, pharmacists found improve patients' scores, asthma. Keywords: techniques,

Язык: Английский

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Knowledge and Practice of Healthcare Professionals in the Medical Care of Asthma Adult Patients in Jordan with a Particular Reference to Adherence to GINA Recommendations

Journal of Multidisciplinary Healthcare, Год журнала: 2024, Номер Volume 17, С. 391 - 404

Опубликована: Янв. 1, 2024

Purpose: This study aimed to evaluate Jordanian healthcare professionals' knowledge, skills, and experience in dealing with treating adult asthmatic patients assess compliance the Global Initiative for Asthma (GINA) guideline recommendations asthma treatment management Jordan. Patients Methods: A cross-sectional survey was conducted Jordan between May June 2023. included physicians, pharmacists, nurses working clinical settings directly interacting Jordan's private governmental facilities who were eligible participate this research. Three primary sections of an online constructed using Google Forms demographic data, a diagnosis illness, list worldwide medical references asthma. Results: total 300 professionals, including pharmacists (103), (87), Doctor Pharmacy (28), physicians (82), took part The respondents' general knowledge nature symptoms is quite high, exceeding 84% 86%, respectively. also showed high degree awareness diagnosis. It revealed that around one-quarter participants consistently reported severity most frequent factor influencing decision take controller or maintenance medicines symptom (reported from 86% participants). GINA guidelines selected as information source by almost half participants. Finally, 47% aware GINA's 2019 adjustments recommendations. Conclusion: professionals understand nature, symptoms, diagnoses disease. However, many lack adherence, awareness, understanding about management. More research required adherence evidence-based impact training courses. Keywords: asthma, GINA, healthcare, practitioners

Язык: Английский

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Investigating the human rhinovirus co-infection in patients with asthma exacerbations and COVID-19

Pharmacy Practice, Год журнала: 2022, Номер 20(2), С. 01 - 10

Опубликована: Июль 10, 2022

To investigate the co-infections with human rhinovirus (HRV) among patients asthma exacerbation and COVID-19 in Jordan. Also, to determine frequency of acute before during pandemic on a matched basis.The data this prospective cohort research consisted clinical variables. During first visit, after 14-days, nasopharyngeal swabs were taken quantitative polymerase chain reaction was performed for HRV SARS-CoV-2 detection.Forty-seven out 175 (26.9%) adult cases have been diagnosed asthma. The number exacerbations study participants higher 2021 than 2020 (p=0.035). Most included asthmatic (61.7%) only positive 38.3% co-infected HRV. cycle threshold value lower samples infected both viruses compared alone, p<0.005.Our findings indicate that significantly more prevalent stable Thus, and/or infections potentially cofactors or contributors cohort. This is study, Jordan, co-infection could be related severity COVID-19.

Язык: Английский

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Molecular Detection of Antibiotic Resistance Genes Using Respiratory Sample from Pneumonia Patients

Antibiotics, Год журнала: 2025, Номер 14(5), С. 502 - 502

Опубликована: Май 13, 2025

Antibiotic resistance makes the treatment of pneumonia challenging. Effective management depends on accurate diagnostic techniques to identify genes and customize drugs. This study primarily aimed antibiotic in respiratory samples from patients with using polymerase chain reaction (PCR) determine prevalence specific analyze clinical factors contributing resistance, as well provide actionable insights into patterns Jordan support efforts improve management. retrospective observational included 114 who were diagnosed pneumonia. Clinical data, including prior exposure history, collected. PCR diagnostics used detect samples. In this study, we evaluated 14 pathogens, highlighting their diverse mechanisms. Mec A was most frequently detected gene, appearing 87 (77.3%). Additionally, Tem 80 (70.2%), Oxa-48-like 15 (13.2%), Ctx-M-1 38 (33.3%) among commonly genes. contrast, Oxa-40-like (7.0%), Vim (8.8%), Imp (4.4%) exhibited a lower prevalence. The Oxa-51-like gene showed only significant association ertapenem (p-value = 0.046). Further analysis revealed statistically associations between methicillin (p < 0.001), underscoring its critical role. However, other genes, such Oxa-48-like, no correlation imipenem meropenem > 0.05). demonstrates utility PCR-based for detecting highlights associated These findings underscore importance integrating molecular routine care outcomes combat growing threat Jordan. PCR's value guiding effective strategies addressing multidrug-resistant

Язык: Английский

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Co‑infection of COVID-19 patients with atypical bacteria: A study based in Jordan

Pharmacy Practice, Год журнала: 2023, Номер 21(1), С. 01 - 05

Опубликована: Апрель 5, 2023

The aim of this work was to know the prevalence Chlamydophila pneumoniae and Mycoplasma in coronavirus disease 2019 (COVID-19) patients Jordan. Also, assess a TaqMan real-time polymerase chain reaction (PCR) assay detecting these two bacteria.This is retrospective study performed over last five months 2021. All nasopharyngeal specimens from COVID-19 were tested for C. pneumonia, M. pneumoniae. Pst-1 gene P1 cytadhesin protein targets.In study, 14 out 175 individuals with confirmed severe acute respiratory syndrome 2 (SARS-CoV-2) infection (8.0%) co-infected or Co-infection SARS-CoV-2 reported 5 (2.9%) patients, while 9 (5.1%) had co-infection. mean (± std) correlation coefficient calibration curve PCR analysis -0.993 0.001) -0.994 0.003) amplification efficiencies Pneumoniae 187.62% 136.86%, respectively.In first based Jordan, infected have low rate atypical bacterial However, clinicians should suspect co-infections both common uncommon bacteria patients. Large prospective investigations are needed give additional insight on true their impact clinical course

Язык: Английский

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Etiologies of Acute Bronchiolitis in Children at Risk for Asthma, with Emphasis on the Human Rhinovirus Genotyping Protocol

Journal of Clinical Medicine, Год журнала: 2023, Номер 12(12), С. 3909 - 3909

Опубликована: Июнь 8, 2023

This research aims to determine acute bronchiolitis’ causative virus(es) and establish a viable protocol classify the Human Rhinovirus (HRV) species. During 2021–2022, we included children 1–24 months of age with bronchiolitis at risk for asthma. The nasopharyngeal samples were taken subjected quantitative polymerase chain reaction (qPCR) in viral panel. For HRV-positive samples, high-throughput assay was applied, directing VP4/VP2 VP3/VP1 regions confirm BLAST searching, phylogenetic analysis, sequence divergence took place identify degree which these appropriate identifying differentiating HRV. HRV ranked second, following RSV, as etiology children. conclusion investigation all available data this study distributed sequences into 7 HRV-A, 1 HRV-B, HRV-C types based on sequences. nucleotide between clinical corresponding reference strains lower region than region. results demonstrated potential utility genotypes. Confirmatory outcomes yielded, indicating how nested semi-nested PCR can practical ways facilitate sequencing genotyping.

Язык: Английский

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