In Vitro Cellular & Developmental Biology - Animal,
Год журнала:
2021,
Номер
57(8), С. 763 - 774
Опубликована: Сен. 1, 2021
Diabetic
kidney
disease
(DKD)
has
become
the
most
common
cause
of
chronic
disease.
Proteinuria
is
generally
considered
one
clinical
indicators
renal
damage,
and
it
also
closely
related
to
progression
DKD.
Accumulating
evidence
indicates
that
proteinuria
induces
an
upregulation
expression
levels
inflammatory
cytokines
fibrosis
markers
in
tubular
epithelial
cells,
but
mechanism
remains
unclear.
Previously,
we
showed
early
growth
response
1
(Egr1)
played
a
key
role
injury.
However,
upstream
Egr1
development
DKD
poorly
understood.
In
this
study,
found
albumin
stimulation
significantly
increased
Egr1,
interleukin
6
(IL-6),
tumor
necrosis
factor-α
(TNF-α),
fibronectin
(FN)
HK-2
cells
decreased
miR-23a-3p
levels.
We
then
identified
targeted
3'
untranslated
region
(UTR)
directly
suppressed
Egr1.
Moreover,
overexpression
inhibition
attenuated
promoted
IL-6,
TNF-α,
FN,
respectively.
Additionally,
silencing
reversed
inflammation
caused
by
inhibitor.
Thus,
conclude
attenuates
through
suggesting
targeting
may
be
novel
therapeutic
approach
for
Cells,
Год журнала:
2020,
Номер
9(7), С. 1574 - 1574
Опубликована: Июнь 28, 2020
MicroRNAs
(miRNAs),
a
class
of
small
non-coding
RNA
molecules,
are
responsible
for
silencing
and
post-transcriptional
regulation
gene
expression.
They
can
mediate
fine-tuned
crosstalk
among
coding
molecules
sharing
miRNA
response
elements
(MREs).
In
suitable
environment,
both
be
targeted
by
the
same
miRNAs
indirectly
regulate
each
other
competing
them.
These
RNAs,
otherwise
known
as
endogenous
RNAs
(ceRNAs),
lead
to
an
additional
regulatory
layer,
where
find
new
significance.
The
miRNA-mediated
interplay
different
types
has
been
observed
in
many
contexts.
analyses
ceRNA
networks
cancer
pathologies,
well
physiological
conditions,
provide
opportunities
interpreting
omics
data
field
personalized
medicine.
development
novel
computational
tools,
providing
putative
predictions
interactions,
is
rapidly
growing
interest.
this
review,
I
discuss
present
current
knowledge
mechanism
its
implications
broad
spectrum
such
cardiovascular
or
autoimmune
diseases,
cancers
neurodegenerative
disorders.
Diabetes Metabolic Syndrome and Obesity,
Год журнала:
2019,
Номер
Volume 12, С. 2609 - 2617
Опубликована: Дек. 1, 2019
Background:
Diabetic
nephropathy
(DN)
is
the
leading
cause
of
end-stage
renal
failure
worldwide.
lncRNAs
are
demonstrated
to
improve
DN
by
changing
expression
miRNAs.
This
study
was
aimed
investigate
effect
lncRNA
GAS5/miR-452-5p
on
inflammation,
oxidative
stress
and
pyroptosis
high-glucose-induced
tubular
cells.
Methods:
HK-2
cells
were
induced
HG
simulate
RT-qPCR
analysis
confirmed
transfection
effects
detected
GAS5,
NLRP3,
caspase1,
IL-1β,
pro-caspase1,
pro-IL-1β,
GSDMD-N
miR-452-5p.
Western
blot
determined
protein
pro-IL-1β
GSDMD-N.
The
also
verified
immunofluorescence.
levels
TNF-α,
IL-6,
MCP-1,
ROS,
MDA
SOD
measured
commercial
assay
kits,
respectively.
Dual-luciferase
reporter
indicated
that
GAS5
could
combine
with
Results:
decreased
in
HG-induced
overexpression
decrease
ROS
increase
SOD.
Moreover,
suppressed
IL-1β
GSDMD-N,
results
immunofluorescence
above
results.
miR-452-5p
interference
same
changes
as
for
cells,
inhibition
reverse
interference.
Conclusion:
inhibited
downregulating
Keywords:
miR-452-5p,
stress,
pyroptosis,
high
glucose,
Infection and Chemotherapy,
Год журнала:
2020,
Номер
52(1), С. 1 - 1
Опубликована: Янв. 1, 2020
Sepsis
is
a
dysregulated
systemic
reaction
to
common
infection,
that
can
cause
lifethreatening
organ
dysfunction.Over
the
last
decade,
mortality
rate
of
patients
with
sepsis
has
decreased
as
long
are
treated
according
recommendations
Surviving
Campaign,
but
still
unacceptably
high.Patients
at
risk
should
therefore
be
identified
prior
onset
dysfunction
and
this
requires
rapid
diagnosis
prompt
initiation
treatment.Unfortunately,
there
no
gold
standard
for
traditional
culture
methods
time-consuming.Recently,
in
order
overcome
these
limitations,
biomarkers
which
could
help
predicting
prognosis
sepsis,
well
being
useful
monitoring
response
treatments,
have
been
identified.In
addition,
recent
advances
led
development
newly
classes
such
microRNAs,
long-non-coding
RNAs,
human
microbiome.This
review
focuses
on
latest
information
used
predict
sepsis.
Clinical Epigenetics,
Год журнала:
2021,
Номер
13(1)
Опубликована: Апрель 21, 2021
Abstract
Diabetic
kidney
disease
(DKD)
is
one
of
the
most
common
microvascular
complication
both
type
1
(T1DM)
and
2
diabetes
mellitus
(T2DM),
leading
cause
end-stage
renal
(ESRD)
worldwide.
Persistent
inflammation
subsequent
chronic
fibrosis
are
major
causes
loss
function,
which
associated
with
progression
DKD
to
ESRD.
In
fact,
affected
by
a
combination
genetic
environmental
factors.
Approximately,
one-third
diabetic
patients
progress
develop
despite
intensive
glycemic
control,
propose
an
essential
concept
“metabolic
memory.”
Epigenetic
modifications,
extensively
studied
mechanism
metabolic
memory,
have
been
shown
contribute
susceptibility
DKD.
modifications
also
play
regulatory
role
in
interactions
between
genes
The
epigenetic
contributions
processes
fibrogenesis
involved
occur
at
different
levels,
including
DNA
methylation,
histone
modification
non-coding
RNA
modulation.
Compared
factors,
epigenetics
represents
new
therapeutic
frontier
understanding
development
may
lead
breakthroughs
due
possibility
reverse
these
therapeutically.
Early
recognition
events
biomarkers
crucial
for
timely
diagnosis
intervention
DKD,
prevention
Herein,
we
will
review
latest
mechanisms
pathology
(T1DN)
nephropathy
(T2DN)
highlight
emerging
possible
strategies
based
on
DKD-associated
fibrogenesis.
Journal of Diabetes Research,
Год журнала:
2020,
Номер
2020, С. 1 - 10
Опубликована: Июнь 22, 2020
The
correlation
between
diabetes
and
systematic
well-being
on
human
life
has
long
established.
As
a
common
complication
of
diabetes,
the
prevalence
diabetic
nephropathy
(DN)
been
increasing
globally.
DN
is
known
to
be
major
cause
end-stage
kidney
disease
(ESKD).
Till
now,
molecular
mechanisms
for
have
not
fully
explored
effective
therapies
are
still
lacking.
Noncoding
RNAs
class
produced
by
genome
transcription
that
cannot
translated
into
proteins.
It
documented
ncRNAs
participate
in
pathogenesis
regulating
inflammation,
apoptosis,
autophagy,
cell
proliferation,
other
pathological
processes.
In
this
review,
roles
diagnostic
therapeutic
potential
three
types
(microRNA,
noncoding
RNA,
circular
RNA)
progression
summarized
illustrated.
Frontiers in Pharmacology,
Год журнала:
2021,
Номер
11
Опубликована: Янв. 26, 2021
Diabetic
kidney
disease
(DKD)
is
the
most
common
diabetic
complication
and
a
leading
cause
of
end-stage
disease.
Increasing
evidence
shows
that
DKD
regulated
not
only
by
many
classical
signaling
pathways
but
also
epigenetic
mechanisms
involving
chromatin
histone
modifications,
DNA
methylation,
non-coding
RNA
(ncRNAs).
In
this
review,
we
focus
on
our
current
understanding
role
ncRNAs,
including
microRNAs
(miRNAs)
long
RNAs
(lncRNAs)
in
pathogenesis
DKD.
Of
them,
regulatory
TGF-β/Smad3-dependent
miRNAs
lncRNAs
highlighted.
Importantly,
as
biomarkers
therapeutic
targets
for
are
described,
perspective
ncRNAs
novel
approach
combating
nephropathy
discussed.
Frontiers in Physiology,
Год журнала:
2021,
Номер
12
Опубликована: Май 5, 2021
Diabetic
nephropathy
(DN)
leads
to
high
morbidity
and
disability.
Inflammation
plays
a
critical
role
in
the
pathogenesis
of
DN,
which
involves
renal
cells
immune
cells,
microenvironment,
as
well
extrinsic
factors,
such
hyperglycemia,
chemokines,
cytokines,
growth
factors.
Epigenetic
modifications
usually
regulate
gene
expression
via
DNA
methylation,
histone
modification,
non-coding
RNAs
without
altering
sequence.
During
past
years,
numerous
studies
have
been
published
reveal
mechanisms
epigenetic
that
inflammation
DN.
This
review
aimed
summarize
latest
evidence
on
interplay
epigenetics
highlight
potential
targets
for
treatment
diagnosis
Frontiers in Physiology,
Год журнала:
2021,
Номер
12
Опубликована: Апрель 29, 2021
Background
and
Objective:
Acute
kidney
injury
(AKI)
is
a
complication
of
sepsis.
Pyroptosis
gasdermin
D
(GSDMD)-mediated
tubular
epithelial
cells
(TECs)
play
important
roles
in
pathogenesis
sepsis-associated
AKI.
Long
non-coding
RNA
(lncRNA)
maternally
expressed
gene
3
(
MEG3
),
an
imprinted
involved
tumorigenesis,
implicated
pyroptosis
occurring
multiple
organs.
Herein,
we
investigated
the
role
mechanisms
regulation
TEC
lipopolysaccharide
(LPS)-induced
Materials
Methods:
Male
C57BL/6
mice
primary
human
TECs
were
treated
with
LPS
for
24
h
to
establish
animal
cell
models,
respectively,
sepsis-induced
Renal
function
was
assessed
by
evaluation
serum
creatinine
urea
levels.
tubule
score
Periodic
acid-Schiff
staining.
evaluating
expression
caspase-1,
GSDMD,
inflammatory
factors
IL-1β
IL-18.
Cellular
analyzing
release
rate
LDH,
IL-1β,
IL-18,
using
EtBr
EthD2
renal
tissues
detected
RT-qPCR.
The
molecular
LPS-induced
AKI
through
bioinformatics
analysis,
RNA-binding
protein
immunoprecipitation,
dual
luciferase
reporter
assays,
rescue
experiment.
Results:
both
these
models
significantly
up-regulated.
-knockdown
showed
decreased
number
pyroptotic
cells,
down-regulated
secretion
compared
those
controls;
however,
there
no
difference
caspase-1
between
knockdown
controls.
Bioinformatics
analysis
screened
out
miR-18a-3P,
further
experiments
demonstrated
that
controls
GSDMD
acting
as
ceRNA
miR-18a-3P
promote
pyroptosis.
Conclusion:
Our
study
demonstrates
lncRNA
promoted
regulating
miR-18a-3p/GSDMD
pathway