Competing Endogenous RNAs, Non-Coding RNAs and Diseases: An Intertwined Story

Cells, Год журнала: 2020, Номер 9(7), С. 1574 - 1574

Опубликована: Июнь 28, 2020

MicroRNAs (miRNAs), a class of small non-coding RNA molecules, are responsible for silencing and post-transcriptional regulation gene expression. They can mediate fine-tuned crosstalk among coding molecules sharing miRNA response elements (MREs). In suitable environment, both be targeted by the same miRNAs indirectly regulate each other competing them. These RNAs, otherwise known as endogenous RNAs (ceRNAs), lead to an additional regulatory layer, where find new significance. The miRNA-mediated interplay different types has been observed in many contexts. analyses ceRNA networks cancer pathologies, well physiological conditions, provide opportunities interpreting omics data field personalized medicine. development novel computational tools, providing putative predictions interactions, is rapidly growing interest. this review, I discuss present current knowledge mechanism its implications broad spectrum such cardiovascular or autoimmune diseases, cancers neurodegenerative disorders.

Язык: Английский

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<p>lncRNA GAS5/miR-452-5p Reduces Oxidative Stress and Pyroptosis of High-Glucose-Stimulated Renal Tubular Cells</p>

Diabetes Metabolic Syndrome and Obesity, Год журнала: 2019, Номер Volume 12, С. 2609 - 2617

Опубликована: Дек. 1, 2019

Background: Diabetic nephropathy (DN) is the leading cause of end-stage renal failure worldwide. lncRNAs are demonstrated to improve DN by changing expression miRNAs. This study was aimed investigate effect lncRNA GAS5/miR-452-5p on inflammation, oxidative stress and pyroptosis high-glucose-induced tubular cells. Methods: HK-2 cells were induced HG simulate RT-qPCR analysis confirmed transfection effects detected GAS5, NLRP3, caspase1, IL-1β, pro-caspase1, pro-IL-1β, GSDMD-N miR-452-5p. Western blot determined protein pro-IL-1β GSDMD-N. The also verified immunofluorescence. levels TNF-α, IL-6, MCP-1, ROS, MDA SOD measured commercial assay kits, respectively. Dual-luciferase reporter indicated that GAS5 could combine with Results: decreased in HG-induced overexpression decrease ROS increase SOD. Moreover, suppressed IL-1β GSDMD-N, results immunofluorescence above results. miR-452-5p interference same changes as for cells, inhibition reverse interference. Conclusion: inhibited downregulating Keywords: miR-452-5p, stress, pyroptosis, high glucose,

Язык: Английский

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An Update on Sepsis Biomarkers

Infection and Chemotherapy, Год журнала: 2020, Номер 52(1), С. 1 - 1

Опубликована: Янв. 1, 2020

Sepsis is a dysregulated systemic reaction to common infection, that can cause lifethreatening organ dysfunction.Over the last decade, mortality rate of patients with sepsis has decreased as long are treated according recommendations Surviving Campaign, but still unacceptably high.Patients at risk should therefore be identified prior onset dysfunction and this requires rapid diagnosis prompt initiation treatment.Unfortunately, there no gold standard for traditional culture methods time-consuming.Recently, in order overcome these limitations, biomarkers which could help predicting prognosis sepsis, well being useful monitoring response treatments, have been identified.In addition, recent advances led development newly classes such microRNAs, long-non-coding RNAs, human microbiome.This review focuses on latest information used predict sepsis.

Язык: Английский

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microRNA-181 serves as a dual-role regulator in the development of human cancers

Free Radical Biology and Medicine, Год журнала: 2019, Номер 152, С. 432 - 454

Опубликована: Дек. 30, 2019

Язык: Английский

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Effects of metabolic memory on inflammation and fibrosis associated with diabetic kidney disease: an epigenetic perspective

Clinical Epigenetics, Год журнала: 2021, Номер 13(1)

Опубликована: Апрель 21, 2021

Abstract Diabetic kidney disease (DKD) is one of the most common microvascular complication both type 1 (T1DM) and 2 diabetes mellitus (T2DM), leading cause end-stage renal (ESRD) worldwide. Persistent inflammation subsequent chronic fibrosis are major causes loss function, which associated with progression DKD to ESRD. In fact, affected by a combination genetic environmental factors. Approximately, one-third diabetic patients progress develop despite intensive glycemic control, propose an essential concept “metabolic memory.” Epigenetic modifications, extensively studied mechanism metabolic memory, have been shown contribute susceptibility DKD. modifications also play regulatory role in interactions between genes The epigenetic contributions processes fibrogenesis involved occur at different levels, including DNA methylation, histone modification non-coding RNA modulation. Compared factors, epigenetics represents new therapeutic frontier understanding development may lead breakthroughs due possibility reverse these therapeutically. Early recognition events biomarkers crucial for timely diagnosis intervention DKD, prevention Herein, we will review latest mechanisms pathology (T1DN) nephropathy (T2DN) highlight emerging possible strategies based on DKD-associated fibrogenesis.

Язык: Английский

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Long non-coding RNA MEG3 regulates CSE-induced apoptosis and inflammation via regulating miR-218 in 16HBE cells

Biochemical and Biophysical Research Communications, Год журнала: 2019, Номер 521(2), С. 368 - 374

Опубликована: Окт. 24, 2019

Язык: Английский

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Noncoding RNAs in Diabetic Nephropathy: Pathogenesis, Biomarkers, and Therapy

Journal of Diabetes Research, Год журнала: 2020, Номер 2020, С. 1 - 10

Опубликована: Июнь 22, 2020

The correlation between diabetes and systematic well-being on human life has long established. As a common complication of diabetes, the prevalence diabetic nephropathy (DN) been increasing globally. DN is known to be major cause end-stage kidney disease (ESKD). Till now, molecular mechanisms for have not fully explored effective therapies are still lacking. Noncoding RNAs class produced by genome transcription that cannot translated into proteins. It documented ncRNAs participate in pathogenesis regulating inflammation, apoptosis, autophagy, cell proliferation, other pathological processes. In this review, roles diagnostic therapeutic potential three types (microRNA, noncoding RNA, circular RNA) progression summarized illustrated.

Язык: Английский

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Non-Coding RNAs as Biomarkers and Therapeutic Targets for Diabetic Kidney Disease

Frontiers in Pharmacology, Год журнала: 2021, Номер 11

Опубликована: Янв. 26, 2021

Diabetic kidney disease (DKD) is the most common diabetic complication and a leading cause of end-stage disease. Increasing evidence shows that DKD regulated not only by many classical signaling pathways but also epigenetic mechanisms involving chromatin histone modifications, DNA methylation, non-coding RNA (ncRNAs). In this review, we focus on our current understanding role ncRNAs, including microRNAs (miRNAs) long RNAs (lncRNAs) in pathogenesis DKD. Of them, regulatory TGF-β/Smad3-dependent miRNAs lncRNAs highlighted. Importantly, as biomarkers therapeutic targets for are described, perspective ncRNAs novel approach combating nephropathy discussed.

Язык: Английский

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Epigenetics and Inflammation in Diabetic Nephropathy

Frontiers in Physiology, Год журнала: 2021, Номер 12

Опубликована: Май 5, 2021

Diabetic nephropathy (DN) leads to high morbidity and disability. Inflammation plays a critical role in the pathogenesis of DN, which involves renal cells immune cells, microenvironment, as well extrinsic factors, such hyperglycemia, chemokines, cytokines, growth factors. Epigenetic modifications usually regulate gene expression via DNA methylation, histone modification, non-coding RNAs without altering sequence. During past years, numerous studies have been published reveal mechanisms epigenetic that inflammation DN. This review aimed summarize latest evidence on interplay epigenetics highlight potential targets for treatment diagnosis

Язык: Английский

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Long Non-coding RNA MEG3 Promotes Renal Tubular Epithelial Cell Pyroptosis by Regulating the miR-18a-3p/GSDMD Pathway in Lipopolysaccharide-Induced Acute Kidney Injury

Frontiers in Physiology, Год журнала: 2021, Номер 12

Опубликована: Апрель 29, 2021

Background and Objective: Acute kidney injury (AKI) is a complication of sepsis. Pyroptosis gasdermin D (GSDMD)-mediated tubular epithelial cells (TECs) play important roles in pathogenesis sepsis-associated AKI. Long non-coding RNA (lncRNA) maternally expressed gene 3 ( MEG3 ), an imprinted involved tumorigenesis, implicated pyroptosis occurring multiple organs. Herein, we investigated the role mechanisms regulation TEC lipopolysaccharide (LPS)-induced Materials Methods: Male C57BL/6 mice primary human TECs were treated with LPS for 24 h to establish animal cell models, respectively, sepsis-induced Renal function was assessed by evaluation serum creatinine urea levels. tubule score Periodic acid-Schiff staining. evaluating expression caspase-1, GSDMD, inflammatory factors IL-1β IL-18. Cellular analyzing release rate LDH, IL-1β, IL-18, using EtBr EthD2 renal tissues detected RT-qPCR. The molecular LPS-induced AKI through bioinformatics analysis, RNA-binding protein immunoprecipitation, dual luciferase reporter assays, rescue experiment. Results: both these models significantly up-regulated. -knockdown showed decreased number pyroptotic cells, down-regulated secretion compared those controls; however, there no difference caspase-1 between knockdown controls. Bioinformatics analysis screened out miR-18a-3P, further experiments demonstrated that controls GSDMD acting as ceRNA miR-18a-3P promote pyroptosis. Conclusion: Our study demonstrates lncRNA promoted regulating miR-18a-3p/GSDMD pathway

Язык: Английский

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