N7-methyladenosine-induced SLC7A7 serves as a prognostic biomarker in pan-cancer and promotes CRC progression in colorectal cancer DOI Creative Commons
Fuqi Wang,

Shiqian Zhang,

Zhuang Chen

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Дек. 28, 2024

Abstract Solute transport family 7A member 7 (SLC7A7) mutations contribute to lysinuric protein intolerance (LPI), which is the mechanism of action that has been extensively studied. In colorectal cancer (CRC), SLC7A7 appears play a role, but features and mechanisms are not yet well understood. Survival was analyzed using Kaplan–Meier analysis. Enrichment analysis performed characterize, immune infiltration, methylation, genetic instability, crucial pathways SLC7A7. Afterward, functional experiments were conducted in vitro investigate how affects tumor metastasis. Mechanistically, quantitative real-time PCR (qRT-PCR), western blot (WB), methylated RNA immunoprecipitation (me-RIP) carried out confirm methylation modification related functions. High levels expression predictive worse prognosis for CRC patients. showed significantly enriched during EMT could be Wnt/β-catenin signaling pathway, infiltration pan-cancer macrophages, affected specific cancers. indicated promote migration invasion cells experiments. observed potentially interact with possibly by influencing adenomatous polyposis coli (APC) expression. Furthermore, we identified undergoes N7-methylguanosine (m7G) modification, may regulate mRNA stability, Quaking (QKI) playing role this process recognizing m7G modification. Our results indicate metastasis through SLC7A7/APC/Wnt/β-catenin pathway. Moreover, might involved regulating highlighting novel layer regulation.

Язык: Английский

Relationship between smoking status and ulcerative colitis: a meta-analysis based on a case–control study DOI Creative Commons

Mi Yong,

Yue Tan, Yangyang R. Yu

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 17, 2025

Prior research has revealed an association between smoking, reduced ulcerative colitis (UC) risk, and improved disease severity among individuals with existing UC. Herein, we analysed the interaction smoking habits UC its potential complexity, focusing on associations incidence of UC, therapeutic outcomes, via a meta-analysis data from case-control studies. The PubMed, China Knowledge, WanFang, Web Science, Science Technology Journal, Embase, Scopus databases were searched, yielding nine studies investigating progression for inclusion. Compared to non-smoking or cessation, had protective effect against indicating that it may reduce risk developing UC; specifically, was associated significant compared cessation (pooled odds ratio (OR): 0.26, 95% confidence interval (CI): 0.21-0.32; P = 0.067, I2 45.2%). Non-smoking also OR: 1.84, CI 1.13-3.30; 0.935, 0%). Further, significantly more than 0.48, 0.40-0.56; 0.002, 66.4%). These results indicate offers some protection onset in patients

Язык: Английский

Процитировано

0

Molecular subtypes of ischemic heart disease based on circadian rhythm DOI Creative Commons
Zhaokai Zhou, Ge Zhang, Zhan Wang

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Июнь 19, 2024

Abstract Coronary atherosclerotic heart disease (CAD) is among the most prevalent chronic diseases globally. Circadian rhythm disruption (CRD) closely associated with progression of various diseases. However, precise role CRD in development CAD remains to be elucidated. The score (CRDscore) was employed quantitatively assess level samples. Our investigation revealed a significant association between high CRDscore and adverse prognosis patients, along substantial correlation progression. Remarkably distinct distributions were also identified subtypes. In summary, we have pioneered revelation relationship at single-cell established reliable markers for development, treatment, CAD. A deeper understanding these mechanisms may offer new possibilities incorporating "the therapy coronary based circadian rhythm" into personalized medical treatment regimens.

Язык: Английский

Процитировано

1

Assessing personalized molecular portraits underlying endothelial-to-mesenchymal transition within pulmonary arterial hypertension DOI Creative Commons

Ruhao Wu,

Ge Zhang, Mingzhou Guo

и другие.

Molecular Medicine, Год журнала: 2024, Номер 30(1)

Опубликована: Окт. 26, 2024

Abstract Pulmonary arterial hypertension (PAH) is a progressive and rapidly fatal disease with an intricate etiology. Identifying biomarkers for early PAH lesions based on the exploration of subtle biological processes significant timely diagnosis treatment. In present study, nine distinct cell populations identified gene expression profiles revealed high heterogeneity in composition ratio, function, distribution preference, communication patterns PAH. Notably, compared to other cells, endothelial cells (ECs) showed prominent variation multiple perspectives. Further analysis demonstrated endothelial-to-mesenchymal transition (EndMT) ECs subgroup exhibiting contrasting phenotype. Based these findings, machine-learning integrated program consisting learners was developed create Endothelial-to-mesenchymal Signature (PETS). This study underlying EndMT furnished potential tool that might be valuable new precise therapies.

Язык: Английский

Процитировано

0

N7-methyladenosine-induced SLC7A7 serves as a prognostic biomarker in pan-cancer and promotes CRC progression in colorectal cancer DOI Creative Commons
Fuqi Wang,

Shiqian Zhang,

Zhuang Chen

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Дек. 28, 2024

Abstract Solute transport family 7A member 7 (SLC7A7) mutations contribute to lysinuric protein intolerance (LPI), which is the mechanism of action that has been extensively studied. In colorectal cancer (CRC), SLC7A7 appears play a role, but features and mechanisms are not yet well understood. Survival was analyzed using Kaplan–Meier analysis. Enrichment analysis performed characterize, immune infiltration, methylation, genetic instability, crucial pathways SLC7A7. Afterward, functional experiments were conducted in vitro investigate how affects tumor metastasis. Mechanistically, quantitative real-time PCR (qRT-PCR), western blot (WB), methylated RNA immunoprecipitation (me-RIP) carried out confirm methylation modification related functions. High levels expression predictive worse prognosis for CRC patients. showed significantly enriched during EMT could be Wnt/β-catenin signaling pathway, infiltration pan-cancer macrophages, affected specific cancers. indicated promote migration invasion cells experiments. observed potentially interact with possibly by influencing adenomatous polyposis coli (APC) expression. Furthermore, we identified undergoes N7-methylguanosine (m7G) modification, may regulate mRNA stability, Quaking (QKI) playing role this process recognizing m7G modification. Our results indicate metastasis through SLC7A7/APC/Wnt/β-catenin pathway. Moreover, might involved regulating highlighting novel layer regulation.

Язык: Английский

Процитировано

0