Journal of Molecular Structure, Год журнала: 2024, Номер unknown, С. 141273 - 141273
Опубликована: Дек. 1, 2024
Язык: Английский
Clinica Chimica Acta, Год журнала: 2024, Номер 565, С. 119983 - 119983
Опубликована: Окт. 3, 2024
Язык: Английский
Процитировано
15
PLoS ONE, Год журнала: 2025, Номер 20(1), С. e0315435 - e0315435
Опубликована: Янв. 2, 2025
The use of granulocyte colony-stimulating factor (GCSF) to control febrile neutropenia (FN) caused by anti-cancer chemotherapy is well documented but it still needs evaluated with respect the specific type cancer and chemotherapeutic agents. present study evaluates efficacy adjunctive GCSF for treating FN after taking anticancer therapy measuring clinical, hematological microbiological outcomes. It a single center conducted at Hayatabad Medical Complex (HMC), Peshawar, Pakistan. Adult patients both genders, suffering from different types sarcomas were included in study. was between January 2023 2024. Baseline data including demographic data, medication history evaluation all recorded time enrolment. Primary outcomes extent absolute neutrophil count (ANC) recovery, duration severity (grade IV), period fever resolution. After (with without GCSF) clinical outcomes, compared evaluated. All statistically analyzed SPSS (IBMS, version 20). A total number 120 investigated out which 109 included. Out patients, 64 (58.72%) received therapy, 45 (41.28%) did not receive GCSF. Comparison showed that receiving had significant improvement ANC recovery time, better free infections. This concluded benefits undergoing treatment carcinoma.
Язык: Английский
Процитировано
0
Scientific Reports, Год журнала: 2025, Номер 15(1)
Опубликована: Янв. 16, 2025
The dysregulation of matrix metalloproteinases (MMPs) in skin cutaneous melanoma (SKCM) represents a critical aspect tumorigenesis. In this study, we investigated the diagnostic, prognostic, and therapeutic aspects MMPs SKCM. Thirteen SKCM cell lines seven normal were cultured under standard conditions for experimental analyses. RNA DNA extracted, followed by RT-qPCR to assess MMP expression promoter methylation analysis determine levels. Functional assays, including proliferation, colony formation, wound healing, conducted post-MMP7 knockdown using siRNA A375 cells. Databases like GEPIA2, HPA, MEXPRESS, miRNet employed expression, survival, methylation, miRNA-mRNA network We landscape lines, revealing significant (p-value < 0.05) up-regulation MMP1, MMP7, MMP9, MMP10, MMP11, MMP12, MMP13, MMP14, MMP25, alongside down-regulation MMP2, MMP3, MMP21. Furthermore, our demonstrated inverse correlation between levels status, suggesting potential regulatory role dysregulation. Notably, MMP14 exhibited associations with overall survival patients, emphasizing their prognostic significance. Additionally, Receiver operating characteristic (ROC) curve highlighted diagnostic distinguishing from individuals. Subsequent validation across multiple cohorts confirmed elevated tissues, particularly advanced disease stages, further tumor progression. elucidated pathways involving miR-22-3p, which targets genes Our findings also revealed immune modulation, drug sensitivity, functional states Lastly, MMP7 cells significantly impacted several characteristics, healing. highlight These could serve as biomarkers early detection therapy. Future efforts should focus on preclinical clinical translate these insights into personalized strategies.
Язык: Английский
Процитировано
0
Hereditas, Год журнала: 2025, Номер 162(1)
Опубликована: Фев. 3, 2025
Gastric cancer (GC) presents a significant global health burden, necessitating deeper understanding of its molecular underpinnings for improved diagnostics and therapeutics. In this study, we investigated the expression profiles clinical implications MAP3K genes in GC using silico vitro experiments. Utilizing RT-qPCR analysis, observed up-regulation MAP3K1, MAP3K4, MAP3K5, MAP3K6, MAP3K7, MAP3K8, MAP3K9, MAP3K10 cell lines, while MAP3K2, MAP3K3, MAP3K11, MAP3K12, MAP3K13, MAP3K14, MAP3K15 exhibited down-regulation. Prognostic evaluation revealed that elevated was associated with shorter overall survival (OS), emphasizing their significance. Furthermore, diagnostic potential demonstrated through robust Receiver operating characteristics (ROC) curve indicating strong discriminatory power these distinguishing patients. Proteomic analysis further confirmed higher GC. Methylation profiling supported idea promoter hypomethylation up-regulation. Single-cell functional elucidated involvement shaping tumor microenvironment. miRNA-mRNA network intricate regulatory mechanisms, hsa-mir-200b-3p emerging as key regulator. Finally, MAP3K1 knockdown has shown impacts on cellular behavior BGC823 cells. This comprehensive assessment provides valuable insights into role GC, offering avenues research therapeutic exploration.
Язык: Английский
Процитировано
0
Drug Discovery Today, Год журнала: 2025, Номер unknown, С. 104314 - 104314
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
0
American Journal of Translational Research, Год журнала: 2025, Номер 17(2), С. 1200 - 1222
Опубликована: Янв. 1, 2025
Laminin subunit alpha 3 (LAMA3) has been implicated in various cellular processes relevant to cancer progression, including cell proliferation, migration, and adhesion. In this study, we explored the expression, prognostic significance, functional role of LAMA3 across multiple types. The silico analyses involve using bioinformatics tools databases, such as Cancer Genome Atlas (TCGA), TIMER2.0, GEPIA2, UALCAN, Kaplan-Meier (KM) plotter, GENT2, Human Protein (HPA), OncoDB, Gene Set Analysis (GSCA), TISIDB. vitro include culture, gene knockdown, assays for colony formation, wound healing. Pan-cancer analysis revealed significant variations with upregulation observed cancers pancreatic adenocarcinoma (PAAD) stomach (STAD), downregulation breast (BRCA) colon (COAD). Prognostic indicated high expression correlated poor overall survival (OS) PAAD STAD, whereas low was associated adverse outcomes BRCA. Validation confirmed differential localized primarily endoplasmic reticulum. clinical features BRCA, PAAD, STAD showed consistent trends different stages, races, age groups. Additionally, mutational copy number (CNVs) prevalent heterozygous amplifications deletions STAD. Promoter methylation inversely although were unaffected. Protein-protein interaction (PPI) enrichment LAMA3's involvement ECM-receptor interactions PI3K-Akt signaling, pathways critical cancer. Finally, following knockdown HT-29 cells demonstrated reduced healing, implicating tumor growth metastasis. Overall, these findings suggest that plays a multifaceted tumorigenesis holds potential biomarker therapeutic target cancers.
Язык: Английский
Процитировано
0
Applied Surface Science, Год журнала: 2025, Номер unknown, С. 162843 - 162843
Опубликована: Март 1, 2025
Процитировано
0
Hereditas, Год журнала: 2025, Номер 162(1)
Опубликована: Март 20, 2025
Abstract Background Glioblastoma (GBM) is a highly aggressive brain tumor characterized by poor prognosis and limited therapeutic options. Understanding the molecular mechanisms driving GBM progression essential for developing more effective diagnostic approaches. Specifically, investigating Cell Division Cycle-Associated (CDCA) genes offers new perspectives on cell cycle regulation proliferation of cells, which are key factors in growth resistance to treatment. These have not been extensively studied GBM, making them promising area targeted research potential interventions. This project was launched elucidate pathogenic, diagnostic, roles CDCA GBM. Methodology Total RNA extracted from lines followed RT-qPCR analyze expression genes. The validation, prognostic significance, mutational analysis were performed using various databases. Functional assays, including gene knockdown, colony formation, proliferation, wound healing, conducted U87MG cells assess role CDCA7 CDCA8 Results 12 6 normal revealed significant overexpression these ROC curve demonstrated excellent potential, with AUC values 1 most indicates that effectively distinguishes cells. Validation additional TCGA data confirmed upregulation tumors, association cancer-related pathways. Survival showed higher correlated patients. Mutation, CNV, methylation analyses alterations genes, further supporting their Additionally, linked immune modulation cycle-related functions, suggesting involvement evasion proliferation. Knockdown experiments reduction migration, highlighting as targets. Conclusion Overall, our findings suggest could serve both biomarkers targets
Язык: Английский
Процитировано
0
International Communications in Heat and Mass Transfer, Год журнала: 2025, Номер 164, С. 108895 - 108895
Опубликована: Март 29, 2025
Язык: Английский
Процитировано
0
Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 209 - 225
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0