Advanced Therapeutics,
Год журнала:
2024,
Номер
7(11)
Опубликована: Авг. 24, 2024
Abstract
Extracellular
vesicles
(EVs)
are
membranous
structures
secreted
by
cells
that
play
important
roles
in
intercellular
communication
and
material
transport.
Due
to
its
excellent
biocompatibility,
lipophilicity,
homing
properties,
EVs
have
been
used
as
a
new
generation
of
drug
delivery
systems
for
the
diagnosis
treatment
tumors.
Despite
potential
clinical
benefits
animal‐derived
extracellular
(AEVs),
their
large‐scale
production
remains
sluggish
due
exorbitant
cost
cell
culture,
challenging
quality
control
measures,
limited
capabilities.
This
constraint
significantly
hinders
widespread
application.
Plant‐derived
(PEVs)
share
similar
functionalities
with
AEVs,
yet
they
hold
several
advantages
including
wide
variety
source
materials,
cost‐effectiveness,
ease
preparation,
enhanced
safety,
more
stable
physicochemical
notable
efficacy.
These
merits
position
PEVs
promising
contenders
broad
biomedical
sector.
review
will
elucidate
PEVs,
delineating
therapeutic
mechanisms
cancer
treatment,
explore
prospective
applications
engineered
targeted
nano‐system
drugs,
microRNAs,
small
interfering
RNAs,
beyond.
The
aim
is
heighten
researchers’
focus
on
expedite
progression
from
fundamental
research
transformation
groundbreaking
discoveries.
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Ноя. 26, 2024
Exosomes,
which
are
small
extracellular
vesicles,
of
particular
interest
in
studies
on
SARS-CoV-2
infection
because
their
crucial
role
intercellular
communication.
These
vesicles
released
by
several
cell
types
and
rich
"cargo"
such
as
proteins,
lipids,
nucleic
acids,
vital
for
regulating
immune
response
viral
pathogenesis.
Exosomes
have
been
reported
to
be
involved
transmission,
escape
mechanisms,
illness
development
infection.
This
review
examines
the
current
research
contribution
exosomes
interplay
between
virus
host
cells,
highlighting
potential
diagnostic
biomarkers
therapeutic
targets
combating
COVID-19.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Сен. 15, 2023
Abstract
Clinical
translation
of
gene
therapy
has
been
challenging,
due
to
limitations
in
current
delivery
vehicles
such
as
traditional
viral
vectors.
Herein,
we
report
the
use
gRNA:Cas9
ribonucleoprotein
(RNP)
complexes
engineered
extracellular
vesicles
(EVs)
for
vivo
therapy.
By
leveraging
a
novel
high-throughput
microfluidic
droplet-based
electroporation
system
(μDES),
achieved
10-fold
enhancement
loading
efficiency
and
more
than
1000-fold
increase
processing
throughput
on
RNP
into
EVs
(RNP-EVs),
compared
with
conventional
bulk
electroporation.
The
flow-through
droplets
serve
enormous
bioreactors
offering
millisecond
pulsed,
low-voltage
continuous-flow
scalable
manner,
which
minimizes
Joule
heating
influence
surface
alteration
retain
natural
EV
stability
integrity.
In
Shaker-1
mouse
model
dominant
progressive
hearing
loss,
demonstrated
effective
RNP-EVs
inner
ear
hair
cells,
clear
reduction
Myo7a
sh1
mRNA
expression
RNP-loaded
lipid-like
nanoparticles
(RNP-LNPs),
leading
significant
recovery
measured
by
auditory
brainstem
responses
(ABR).
One
sentence
summary
enables
vesicles,
leads
clinical
potential
employed
disease
Advanced Therapeutics,
Год журнала:
2024,
Номер
7(11)
Опубликована: Авг. 24, 2024
Abstract
Extracellular
vesicles
(EVs)
are
membranous
structures
secreted
by
cells
that
play
important
roles
in
intercellular
communication
and
material
transport.
Due
to
its
excellent
biocompatibility,
lipophilicity,
homing
properties,
EVs
have
been
used
as
a
new
generation
of
drug
delivery
systems
for
the
diagnosis
treatment
tumors.
Despite
potential
clinical
benefits
animal‐derived
extracellular
(AEVs),
their
large‐scale
production
remains
sluggish
due
exorbitant
cost
cell
culture,
challenging
quality
control
measures,
limited
capabilities.
This
constraint
significantly
hinders
widespread
application.
Plant‐derived
(PEVs)
share
similar
functionalities
with
AEVs,
yet
they
hold
several
advantages
including
wide
variety
source
materials,
cost‐effectiveness,
ease
preparation,
enhanced
safety,
more
stable
physicochemical
notable
efficacy.
These
merits
position
PEVs
promising
contenders
broad
biomedical
sector.
review
will
elucidate
PEVs,
delineating
therapeutic
mechanisms
cancer
treatment,
explore
prospective
applications
engineered
targeted
nano‐system
drugs,
microRNAs,
small
interfering
RNAs,
beyond.
The
aim
is
heighten
researchers’
focus
on
expedite
progression
from
fundamental
research
transformation
groundbreaking
discoveries.
