The Common Hallmarks of Aging, Circadian Rhythms and Cancer: Implications for Therapeutic Strategies

Research, Год журнала: 2025, Номер 8

Опубликована: Янв. 1, 2025

The intricate relationship between cancer, circadian rhythms, and aging is increasingly recognized as a critical factor in understanding the mechanisms underlying tumorigenesis cancer progression. Aging well-established primary risk for while disruptions rhythms are intricately associated with progression of various tumors. Moreover, itself disrupts leading to physiological changes that may accelerate development. Despite these connections, specific interplay processes their collective impact on remains inadequately explored literature. In this review, we systematically explore influence We discuss how core genes tumor prognosis, highlighting shared hallmarks such genomic instability, cellular senescence, chronic inflammation. Furthermore, examine aging, focusing crosstalk contributes tumorigenesis, proliferation, apoptosis, well metabolism stability. By elucidating common pathways linking review provides new insights into pathophysiology identifies potential therapeutic strategies. propose targeting regulation could pave way novel treatments, including chronotherapy antiaging interventions, which offer important benefits clinical management cancer.

Язык: Английский

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Ferroptosis and the tumor microenvironment

Journal of Experimental & Clinical Cancer Research, Год журнала: 2024, Номер 43(1)

Опубликована: Ноя. 30, 2024

Abstract Ferroptosis is a type of regulated cell death characterized by its non-apoptotic, iron-dependent and oxidative nature. Since discovery in 2012, extensive research has demonstrated pivotal roles tumorigenesis, metastasis cancer therapy. The tumor microenvironment (TME) complex ecosystem comprising cells, non-cancer extracellular matrix, metabolites cytokines. Recent studies have underscored new paradigm which cells the TME, such as immune stromal also play significant regulating progression therapeutic resistance typically through complicated crosstalk with cells. Notably, this TME were partially mediated ferrotopsis-related mechanisms. This review provides comprehensive systematic summary current findings concerning ferroptosis how ferroptosis-mediated reprogramming impacts progression. Additionally, outlines various ferroptosis-related strategies aimed at targeting TME.

Язык: Английский

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Exosomes as new generation vehicles for drug delivery systems

Journal of Drug Delivery Science and Technology, Год журнала: 2024, Номер 95, С. 105562 - 105562

Опубликована: Март 11, 2024

Язык: Английский

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Electrochemical detection of extracellular vesicles for early diagnosis: a focus on disease biomarker analysis

Extracellular Vesicles and Circulating Nucleic Acids, Год журнала: 2024, Номер 5(2), С. 165 - 79

Опубликована: Апрель 28, 2024

This review article presents a detailed examination of the integral role that electrochemical detection extracellular vesicles (EVs) plays, particularly focusing on potential application for early disease diagnostics through EVs biomarker analysis. Through an exploration benefits and challenges presented by vetted protein, lipid, nucleic acid analysis, we underscore significance these techniques. Evidence from recent studies renders this modality imperative in identifying diverse biomarkers EVs, leading to diagnosis diseases such as cancer neurodegenerative disorders. Recent advancements have led enhanced sensitivity, specificity point-of-care testing (POCT) are elucidated, along with equipment deployed detection. The concludes contemplation future perspectives, recognizing shifts prognosis, necessary advances broad adoption, areas ongoing research. objective is propel further investigation into rapidly burgeoning field, thereby facilitating paradigm shift detection, monitoring, treatment toward human health management.

Язык: Английский

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The systematic role of pancreatic cancer exosomes: distant communication, liquid biopsy and future therapy

Cancer Cell International, Год журнала: 2024, Номер 24(1)

Опубликована: Июль 25, 2024

Abstract Pancreatic cancer remains one of the most lethal diseases worldwide. Cancer-derived exosomes, benefiting from protective role lipid membrane, exhibit remarkable stability in circulatory system. These released by tumor microenvironment, contain various biomolecules such as proteins, RNAs, and lipids that plays a pivotal mediating distant communication between local pancreatic other organs or tissues. They facilitate transfer oncogenic factors to sites, contributing compromised body immune system, metastasis, diabetes, cachexia, promoting microenvironment conducive growth metastasis patients. Beyond their intrinsic roles, circulating exosomes peripheral blood can be detected accurate liquid biopsy. This approach offers novel promising method for diagnosis management cancer. Consequently, are not only crucial mediators systemic cell-cell during progression but also hold great potential precise tools treatment. Exosome-based biopsy therapy represent advancements treatment Exosomes serve drug delivery vehicles, enhancing targeting efficacy anticancer treatments, modulating facilitating gene editing suppress growth. Ongoing research focuses on biomarker identification, systems, clinical trials validate safety exosome-based therapies, offering new possibilities early precision Leveraging therapeutic including ability deliver targeted drugs modulate responses, opens avenues innovative strategies.

Язык: Английский

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Circular RNA landscape in extracellular vesicles from human biofluids

Genome Medicine, Год журнала: 2024, Номер 16(1)

Опубликована: Окт. 31, 2024

Circular RNAs (circRNAs) have emerged as a prominent class of covalently closed single-stranded RNA molecules that exhibit tissue-specific expression and potential biomarkers in extracellular vesicles (EVs) derived from liquid biopsies. Still, their characteristics applications EVs remain to be unveiled. We performed comprehensive analysis EV-derived circRNAs (EV-circRNAs) using transcriptomics data obtained 1082 human body fluids, including plasma, urine, cerebrospinal fluid (CSF), bile. Our validation strategy utilized RT-qPCR immunoprecipitation assays, complemented by computational techniques for analyzing EV-circRNA features RNA-binding protein interactions. identified 136,327 EV-circRNAs various fluids. Significantly, considerable amount with high back-splicing ratio are highly enriched compared linear RNAs. Additionally, we discovered brain-specific plasma cancer-associated linked clinical outcomes. Moreover, demonstrated the serve evaluating immunotherapy efficacy non-small cell lung cancer (NSCLC). Importantly, involvement RBPs, particularly YBX1, sorting mechanism into EVs. This study unveils extensive repertoire across biofluids, offering insights disease mechanistic roles within The identification specific elucidation RBP-mediated mechanisms open new avenues application diagnostics therapeutics.

Язык: Английский

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Diagnostic Utility of a 90-Gene Expression Assay (Canhelp-Origin) for Patients with Metastatic Cancer with an Unclear or Unknown Diagnosis

Molecular Diagnosis & Therapy, Год журнала: 2024, Номер unknown

Опубликована: Сен. 27, 2024

Язык: Английский

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Phloem sap from melon plants contains extracellular vesicles that carry active proteasomes which increase in response to aphid infestation

Journal of Extracellular Vesicles, Год журнала: 2024, Номер 13(10)

Опубликована: Окт. 1, 2024

Abstract The morphogenesis of higher plants requires communication among distant organs throughout vascular tissues (xylem and phloem). Numerous investigations have demonstrated that phloem also act as a distribution route for signalling molecules being observed different macromolecules translocated by the sap, including nucleic acids proteins, change under stress situations. participation extracellular vesicles (EVs) in this has been suggested, although little is known about their role. In fact, last decade, presence EVs originated great controversy, where major concerns arose from origin, isolation methods, even appropriate nomenclature plant nanovesicles. Phloem sap exudates melon plants, either aphid‐free or infested with Aphis gossypii , were collected stem incision. After concentration (Amicon), (PhlEVs) isolated size exclusion chromatography. PhlEVs characterised using Nanoparticle Tracking Analysis, Transmission electron microscopy proteomic analysis. Here we confirm vivo detection changes particles/protein ratio composition response to insect feeding, revealing typical defence proteins cargo well components proteasome complex. showed lower almost two times more proteolytic activity than plants. both cases, such was inhibited dose‐dependent manner inhibitor MG132. Our results suggest may use mechanism prepare themselves receive infectious agents open up possibility an evolutionary conserved against pathogens/stresses eukaryotic organisms.

Язык: Английский

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Identification and evaluation of plasma exosome RNA biomarkers for non-invasive diagnosis of hepatocellular carcinoma using RNA-seq

BMC Cancer, Год журнала: 2024, Номер 24(1)

Опубликована: Дек. 18, 2024

Non-invasive diagnostic methods, including medical imaging techniques and blood biomarkers such as alpha-fetoprotein (AFP), have been crucial in detecting hepatocellular carcinoma (HCC). However, are only effective for tumor size larger than 2 cm. AFP measurement remains unsatisfactory due to high rate of misdiagnosis underdiagnosis. Therefore, new reliable better non-invasive approach necessary HCC identification. The differentially expressed genes were identified using multiple public RNA-seq data liver tissues from healthy individuals patients peritumoral tissues. hub diagnosis combining pathway enrichment analysis protein–protein interaction network analysis. performance was analyzed plasma individuals, HBV infected patients, based on exosomal data. A multi-layer perceptron (MLP) model developed diagnosis. Through differential gene expression datasets, we first 30 dysregulated Protein-protein further narrowed down this list 10 key genes: BRCA2, CDK1, MCM4, PLK1, DNA2, BLM, PCNA, POLD1, BRCA1 FEN1. By evaluation additional tissue POLD1 MCM4 excluded consideration potential their suboptimal performance. Notably, FEN1, PCNA found be significantly elevated the exosomes compared non-HCC those with HBV-infected hepatitis controls. MLP model, three biomarkers, showed an area under curve (AUC) 0.85 0.84 training test dataset respectively, after adjusting covariates sex age. We genes, HCC. utilizing good differentiation between which exhibits promising a tool Additional validation sample is essential thoroughly assess reliability model's

Язык: Английский

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Glycosylation on extracellular vesicles and its detection strategy: Paving the way for clinical use

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 295, С. 139714 - 139714

Опубликована: Янв. 9, 2025

Язык: Английский

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