Frontiers in Immunology, Год журнала: 2024, Номер 15
Опубликована: Окт. 24, 2024
Recent studies have underscored the biological significance of RNA modifications in tumorigenicity and progression. However, potential roles immune regulation formation tumor microenvironment (TME) head neck squamous carcinoma (HNSC) remain unclear.
Язык: Английский
Frontiers in Oncology, Год журнала: 2025, Номер 15
Опубликована: Апрель 7, 2025
Cancer treatment has long been hindered by the complexity of tumor microenvironment (TME) and mechanisms that tumors employ to evade immune detection. Recently, combination checkpoint inhibitors (ICIs) anti-angiogenic therapies emerged as a promising approach improve cancer outcomes. This review delves into role immunostimulatory molecules ICIs in enhancing anti-tumor immunity, while also discussing therapeutic potential strategies cancer. In particular, we highlight critical endoplasmic reticulum (ER) stress angiogenesis. Moreover, explore macrophage reprogramming bolster with focus on restoring phagocytic function, modulating hypoxic environments, targeting cytokines chemokines shape responses. By examining underlying combining therapies, recent clinical trials discuss biomarkers guide predict efficacy.
Язык: Английский
Процитировано
0
Cell Communication and Signaling, Год журнала: 2025, Номер 23(1)
Опубликована: Апрель 29, 2025
Extracellular vesicles (EVs) are nanoscale structures involved in intercellular communication and play a key role cancer pathology. Intrahepatic cholangiocarcinoma (ICC) is highly invasive malignancy marked by abnormal sialylated glycosylation. Analyzing proteins glycans EVs provides insights into ICC molecular subtyping mechanisms. Optimizing EV isolation methods for ICC-derived enables comprehensive proteomic glycomic analysis. We systematically evaluated five methods-Ultracentrifugation (UC), exoEasy, Total Exosome Isolation (TEI), EVtrap, ÄKTA-by analyzing the biophysical properties, profiles, of EVs. Subsequently, we applied TMT-based quantitative proteome light/heavy methylamine labeling quantification N-glycan linkage isomers to investigate alterations N-glycans within secreted HuCCT1 HCCC-9810 cells with overexpressing ST6 β‑galactoside α2,6‑sialyltransferase 1 (ST6GAL1). By evaluating proteome, N-glycome extracted using different methods, UC was identified as optimal approach this study, it offered balance between operational complexity, cost-effectiveness, preservation activity. In total 1,928 high-confidence over 84 were quantified. ST6GAL1 exhibited consistent upregulation 16 proteins, downregulation 10 well 3 glycans. Quantitative analysis revealed that overexpression led significant cell adhesion glycosylation pathways, along specific changes structures. Notably, these modifications extended beyond α2,6-sialylation, suggesting interactions glycosyltransferases may drive alterations.
Язык: Английский
Процитировано
0
Chinese Chemical Letters, Год журнала: 2025, Номер unknown, С. 111333 - 111333
Опубликована: Май 1, 2025
Язык: Английский
Процитировано
0
Journal of Extracellular Vesicles, Год журнала: 2024, Номер 13(10)
Опубликована: Окт. 1, 2024
Abstract The morphogenesis of higher plants requires communication among distant organs throughout vascular tissues (xylem and phloem). Numerous investigations have demonstrated that phloem also act as a distribution route for signalling molecules being observed different macromolecules translocated by the sap, including nucleic acids proteins, change under stress situations. participation extracellular vesicles (EVs) in this has been suggested, although little is known about their role. In fact, last decade, presence EVs originated great controversy, where major concerns arose from origin, isolation methods, even appropriate nomenclature plant nanovesicles. Phloem sap exudates melon plants, either aphid‐free or infested with Aphis gossypii , were collected stem incision. After concentration (Amicon), (PhlEVs) isolated size exclusion chromatography. PhlEVs characterised using Nanoparticle Tracking Analysis, Transmission electron microscopy proteomic analysis. Here we confirm vivo detection changes particles/protein ratio composition response to insect feeding, revealing typical defence proteins cargo well components proteasome complex. showed lower almost two times more proteolytic activity than plants. both cases, such was inhibited dose‐dependent manner inhibitor MG132. Our results suggest may use mechanism prepare themselves receive infectious agents open up possibility an evolutionary conserved against pathogens/stresses eukaryotic organisms.
Язык: Английский
Процитировано
3
Life Sciences, Год журнала: 2024, Номер 351, С. 122810 - 122810
Опубликована: Июнь 11, 2024
Cardiovascular pathology is the main cause of death in chronic kidney disease (CKD) patients. CKD associated with accumulation uremic toxins bloodstream, and indoxyl sulfate (IS) one most abundant found blood We conducted an vitro study to assess mechanisms underlying IS-induced endothelial dysfunction that could lead cardiovascular diseases. also studied their extracellular vesicles (EVs) owing capacity act as messengers transmit signals through cargo. EVs were characterized by nanoparticle tracking analysis, transmission electron microscopy, flow cytometry, tetraspanin expression. Cell lysates isolated analyzed using liquid chromatography coupled mass spectrometry, followed Gene Set Enrichment Analysis identify altered pathways. Proteomic analysis cells revealed IS causes increase proteins related adipogenesis, inflammation, xenobiotic metabolism a decrease proliferation. Extracellular matrix elements, well myogenesis, response UV irradiation, be downregulated IS-treated EVs. Fatty acid was increased along adipogenesis inflammation observed cells. The treatment expression less Furthermore, from treated may mediate dysfunction, since they present fewer inflammatory factors, radiation.
Язык: Английский
Процитировано
2
Therapeutic Advances in Medical Oncology, Год журнала: 2024, Номер 16
Опубликована: Янв. 1, 2024
Circulating tumor cells (CTCs) are that slough off the primary lesions and extravasate into bloodstream. By forming CTC clusters interacting with other circulating (platelets, NK cells, macrophage, etc.), CTCs able to survive in circulatory system of patients colonize metastatic organs. In recent years, potential diagnosis, prognostic assessment, individualized therapy various types tumors has been gradually explored, while advances biotechnology have made it possible extract from patient blood samples. These biological features provide us new insights cancer vulnerabilities. With advent immunotherapies personalized medicines, disrupting heterotypical interaction between as well direct targeting hold great promise. Pancreatic (PC) is one most malignant cancers, part because early metastasis, difficult limited treatment options. Although there significant for a biomarker impact PC diagnosis therapy, still remain number challenges routine implementation clinical management PC. this review, we summed up progress understanding characteristics exceptional technological provided insight exploiting these developments design future tools improving
Язык: Английский
Процитировано
1
Cell Reports, Год журнала: 2024, Номер 43(11), С. 114893 - 114893
Опубликована: Окт. 23, 2024
Язык: Английский
Процитировано
1
BMC Cancer, Год журнала: 2024, Номер 24(1)
Опубликована: Дек. 27, 2024
Abstract Background Male breast cancer (MBC) is a rare malignancy, but its global incidence has shown notable increase in recent decades. Factors such as limited health literacy, inadequate education, and reluctance to seek medical attention contribute the late-stage diagnosis of most MBC patients. Consequently, there an urgent need for highly specific sensitive diagnostic approach MBC. Methods This retrospective study enrolled 20 patients with 30 surgical or biopsy specimens from August 2020 2023. The 90-gene expression assay was performed determine tissue origin. Predicted tumor types were then compared reference accuracy calculation. differentially expressed genes identified between male female cancer. Result demonstrated overall 96.7% (29/30) when pathological diagnosis. For primary, lymph node metastatic, distant metastatic tumors, accuracies 100% (15/15), 90.9% (10/11), (4/4), respectively. Five ( RPS4Y1 , PI15 AZGP1 PRRX1 AGR2 ) up-regulated, six XIST PIGR SFRP1 PLA2G2A S100A2 CHI3L1 down-regulated Conclusion Our findings highlight promising performance accurately identifying origin Incorporating this into diagnoses potential empower oncologists precision treatment options, ultimately enhancing care outcomes
Язык: Английский
Процитировано
1
Опубликована: Окт. 18, 2024
Filopodia are dynamic adhesive cytoskeletal structures that critical for directional sensing, polarization, cell-cell adhesion, and migration of diverse cell types. also neuronal synapse formation. While rearrangement the actin cytoskeleton is known to be filopodia biogenesis, little about upstream extracellular signals. Here, we identify secreted exosomes as potent regulators Inhibition exosome secretion inhibited formation stabilization in both cancer cells neurons subsequent by neurons. Rescue experiments with purified small large vesicles (EVs) identified exosome-enriched EVs (SEVs) having filopodia-inducing activity. Proteomic analyses cell-derived SEVs TGF-β family coreceptor endoglin a key SEV-enriched cargo regulates filopodia. Cancer levels affected filopodia-dependent behaviors, including metastasis chick embryos 3D collagen gels. As do not express endoglin, performed second proteomics experiment SEV cargoes regulated might promote We discovered single was altered endoglin-KD SEVs, transmembrane protein Thrombospondin Type 1 Domain Containing 7A (THSD7A). further found carry THSD7A add-back sufficient rescue defects exosome-inhibited find induces through activation Rho GTPase, Cdc42. These findings suggest new model formation, triggered carrying THSD7A.
Язык: Английский
Процитировано
0
Опубликована: Окт. 18, 2024
Filopodia are dynamic adhesive cytoskeletal structures that critical for directional sensing, polarization, cell-cell adhesion, and migration of diverse cell types. also neuronal synapse formation. While rearrangement the actin cytoskeleton is known to be filopodia biogenesis, little about upstream extracellular signals. Here, we identify secreted exosomes as potent regulators Inhibition exosome secretion inhibited formation stabilization in both cancer cells neurons subsequent by neurons. Rescue experiments with purified small large vesicles (EVs) identified exosome-enriched EVs (SEVs) having filopodia-inducing activity. Proteomic analyses cell-derived SEVs TGF-β family coreceptor endoglin a key SEV-enriched cargo regulates filopodia. Cancer levels affected filopodia-dependent behaviors, including metastasis chick embryos 3D collagen gels. As do not express endoglin, performed second proteomics experiment SEV cargoes regulated might promote We discovered single was altered endoglin-KD SEVs, transmembrane protein Thrombospondin Type 1 Domain Containing 7A (THSD7A). further found carry THSD7A add-back sufficient rescue defects exosome-inhibited find induces through activation Rho GTPase, Cdc42. These findings suggest new model formation, triggered carrying THSD7A.
Язык: Английский
Процитировано
0