Breaking barriers in targeted Therapy: Advancing exosome Isolation, Engineering, and imaging

Advanced Drug Delivery Reviews, Год журнала: 2025, Номер 218, С. 115522 - 115522

Опубликована: Янв. 22, 2025

Язык: Английский

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Why Do Lipid Nanoparticles Target the Liver? Understanding of Biodistribution and Liver-Specific Tropism

Molecular Therapy — Methods & Clinical Development, Год журнала: 2025, Номер 33(1), С. 101436 - 101436

Опубликована: Фев. 16, 2025

Lipid nanoparticles (LNPs) are now highly effective transporters of nucleic acids to the liver. This liver-specificity is largely due their association with certain serum proteins, most notably apolipoprotein E (ApoE), which directs them liver cells by binding low-density lipoprotein (LDL) receptors on hepatocytes. The liver's distinct anatomy, its various specialized cell types, also influences how LNPs taken up from circulation, cleared, and they in delivering treatments. In this review, we consider factors that facilitate LNP's targeting explore latest advances liver-targeted LNP technologies. Understanding targeted can help for design optimization nanoparticle-based therapies. Comprehension cellular interaction biodistribution not only leads better treatments diseases but delivers insight directing other tissues, potentially broadening range therapeutic applications.

Язык: Английский

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The Interplay of Endosomal Escape and RNA Release from Polymeric Nanoparticles

Langmuir, Год журнала: 2025, Номер unknown

Опубликована: Март 13, 2025

Ribonucleic acid (RNA) nanocarriers, specifically lipid nanoparticles and polymeric nanoparticles, enable RNA transfection both in vitro vivo; however, only a small percentage of endocytosed by cell is delivered to the cytosolic machinery, minimizing its effect. nanocarriers face two major obstacles after endocytosis: endosomal escape release. Overcoming simultaneously challenging because usually achieved using high positive charge disrupt membrane. However, this typically also inhibits release anionic strongly bound nanocarrier electrostatic interactions. Many address one over other despite growing body evidence demonstrating that are crucial for transfection. In review, we survey various strategies have been employed accomplish with focus on nanomaterials. We first consider requirements must achieve delivery including protection from degradation, cellular internalization, escape, then discuss current polymers used examine achieving Finally, review stimuli-responsive While continues be challenge efficient transfection, many new innovations materials elucidated promising strategies.

Язык: Английский

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Nano Plasma Membrane Vesicle‐Lipid Nanoparticle Hybrids for Enhanced Gene Delivery and Expression

Advanced Healthcare Materials, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 10, 2024

Lipid nanoparticles (LNPs) have emerged as the leading nonviral nucleic acid (NA) delivery system, gaining widespread attention for their use in COVID-19 vaccines. They are recognized efficient NA encapsulation, modifiability, and scalable production. However, LNPs face efficacy potency limitations due to suboptimal intracellular processing, with endosomal escape efficiencies (ESE) below 2.5%. Additionally, up 70% of NPs undergo recycling exocytosis after cellular uptake. In contrast, cell-derived vesicles offer biocompatibility high-delivery but challenging load exogenous NAs manufacture at large-scale. To leverage strengths both systems, a hybrid system is designed by combining vesicles, such nano plasma membrane (nPMVs), through microfluidic mixing subsequent dialysis. These hybrids demonstrate tenfold increase ESE an 18-fold rise reporter gene expression vitro vivo zebrafish larvae (ZFL) mice, compared traditional LNPs. improvements linked unique physico-chemical properties, composition, morphology. By incorporating this strategy streamlines development process, significantly enhancing systems without need extensive screening.

Язык: Английский

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New insights for the development of efficient DNA vaccines

Microbial Biotechnology, Год журнала: 2024, Номер 17(11)

Опубликована: Ноя. 1, 2024

Abstract Despite the great potential of DNA vaccines for a broad range applications, ranging from prevention infections, over treatment autoimmune and allergic diseases to cancer immunotherapies, implementation such therapies clinical is far behind expectations up now. The main reason poor immunogenicity in humans. Consequently, improvement performance vivo required. This mini‐review provides an overview current state various strategies enhance immunogenic vaccines, including (i) optimization construct itself regarding size, nuclear transfer transcriptional regulation; (ii) use appropriate adjuvants; (iii) improved delivery, example, by careful choice administration route, physical methods as electroporation nanomaterials that may allow cell type‐specific targeting. Moreover, combining nanoformulated with other immunotherapies prime‐boost help success treatment.

Язык: Английский

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Antimicrobial Peptide Delivery Systems as Promising Tools Against Resistant Bacterial Infections

Antibiotics, Год журнала: 2024, Номер 13(11), С. 1042 - 1042

Опубликована: Ноя. 4, 2024

The extensive use of antibiotics during recent years has led to antimicrobial resistance development, a significant threat global public health. It is estimated that around 1.27 million people died worldwide in 2019 due infectious diseases caused by antibiotic-resistant microorganisms, according the WHO. 700,000 die each year worldwide, which expected rise 10 2050. Therefore, new and efficient antimicrobials against resistant pathogenic bacteria are urgently needed. Antimicrobial peptides (AMPs) present broad spectrum antibacterial effects considered potential tools for developing novel therapies combat infections. However, their clinical application currently limited instability, low selectivity, toxicity, bioavailability, resulting narrow therapeutic window. Here we describe an overview AMPs bacterial infections through nanoformulation. evaluates metal, polymeric, lipid AMP delivery systems as promising treatment infections, offering solution aforementioned limitations.

Язык: Английский

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The siEGFR nanoplexes for the enhanced brain glioma treatment: Endoplasmic reticulum biomimetic strategy to induce homing effect and non-degradable intracellular transport

Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 179, С. 117413 - 117413

Опубликована: Сен. 10, 2024

Язык: Английский

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MOVE - how to foster European mobility for early career scientists in EV research

Extracellular Vesicles and Circulating Nucleic Acids, Год журнала: 2024, Номер 5(4), С. 760 - 4

Опубликована: Дек. 12, 2024

Язык: Английский

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