Wastewater based genomic surveillance key to population level monitoring of AmpC/ESBL producing Escherichia coli

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 3, 2025

Язык: Английский

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How to establish a hospital wastewater surveillance program for antimicrobial resistance: Current experience and future knowledge gaps

CMI Communications, Год журнала: 2025, Номер unknown, С. 105087 - 105087

Опубликована: Май 1, 2025

Язык: Английский

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High-Throughput Qpcr Profiling of Antimicrobial Resistance and Bacterial Loads in Wastewater and Receiving Environments: A Risk Assessment

Опубликована: Янв. 1, 2025

Wastewater treatment plants (WWTPs) are hot spots for the acquisition and spread of antimicrobial resistance (AMR). This regional-based study quantified antibiotic genes (ARGs), mobile genetic elements (MGEs), bacteria in hospital community-derived wastewater receiving environments, using high-throughput qPCR (HT-qPCR). is first to apply Resistomap's Antibiotic Resistance Gene Index (ARGI) find overall ARG load AMR risk assessment. ARGI WWTPs ranged from 2.0 2.3, indicating higher relative levels than mean European 2.0, but lower global 2.4. The highest diversity abundance ARGs were observed untreated community wastewater. reduction total during (0.2- 2 logs) (0.3-1.5 varied spatio-temporally across WWTPs. Despite a decrease bacterial treated effluents, substantial loads still released into environments. Notably, coastal sediments comparable those wastewater, most shared between highlighting impact discharge on these ecosystems. Sewage outfall exposure increased shellfish, emphasising risks shellfish hygiene. provides evidence inform policymaking, advanced methods combined storm overflow (CSO) management mitigate release, protecting water users food chain.

Язык: Английский

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High-Throughput qPCR Profiling of Antimicrobial Resistance Genes and Bacterial Loads in Wastewater and Receiving Environments

Environmental Pollution, Год журнала: 2025, Номер unknown, С. 126096 - 126096

Опубликована: Март 1, 2025

Wastewater treatment plants (WWTPs) are hot spots for the acquisition and spread of antimicrobial resistance (AMR). This regional-based study quantified antibiotic genes (ARGs), mobile genetic elements (MGEs), bacteria in hospital community-derived wastewater receiving environments, using high-throughput qPCR (HT-qPCR). is first to apply Resistomap's Antibiotic Resistance Gene Index (ARGI) as a standardised metric find overall AMR level across different WWTPs. ARGI WWTPs ranged from 2.0 2.3, indicating higher relative ARG levels than mean European 2.0, but lower global 2.4. The highest diversity abundance ARGs were observed untreated community wastewater. reduction total during (0.2- 2 logs) (0.3-1.5 varied spatio-temporally Despite decrease bacterial treated effluents, substantial loads still released into environments. Notably, coastal sediments comparable those wastewater, most shared between highlighting impact discharge on these ecosystems. Sewage outfall exposure increased shellfish, emphasising risks shellfish hygiene. provides evidence inform policymaking, advanced methods combined sewer overflow (CSO) management mitigate release, protecting water users food chain.

Язык: Английский

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Drug resistant Klebsiella pneumoniae from patients and hospital effluent: a correlation?

BMC Microbiology, Год журнала: 2025, Номер 25(1)

Опубликована: Май 11, 2025

The application of wastewater-based epidemiology has gained traction as a cost effective tool in antimicrobial resistance (AMR) surveillance with studies showing correlation between the presence resistant bacteria from hospital sewage and patients. This study compared Klebsiella pneumoniae patients effluent terms antibiotic patterns, genes (ARGs), mobile genetic elements (MGEs) phylogenomic relationships. Pooled samples were collected final point regional K. isolates identified on selective media. Clinical also same hospital. Antimicrobial susceptibility testing (AST) was performed using VITEK® 2 system. DNA extracted prior to whole genome sequencing (WGS). resistome, mobilome, phylogenetic lineages sequenced assessed bioinformatics analysis. A total 10 randomly selected presumptive clinical constituted sample subjected AST. Total observed cefuroxime, cefotaxime, piperacillin/tazobactam, gentamicin, tobramycin trimethoprim/sulfamethoxazole. exhibited most antibiotics but showed amoxicillin/clavulanic acid piperacillin/tazobactam (100%), tigecycline (10%). did not exhibit diverse while harboured conferring aminoglycoside (aph(6)-Id, aph(3'')-Ib, aac(6')-Ib-cr, aadA16), ß-lactam (blaSVH group, blaOXA blaTEM group), fluoroquinolone (oqxA, oqxB) antibiotics. Only class 1 integrons identified. Phylogenetic analysis revealed that this closely related isolates. no profiles relationship AMR may depend agents bacterial species studied.

Язык: Английский

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Wastewater surveillance of Candida auris in Baltimore

International Journal of Hygiene and Environmental Health, Год журнала: 2024, Номер 263, С. 114486 - 114486

Опубликована: Ноя. 20, 2024

Язык: Английский

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Metagenomic Meta-Analysis of Antibiotic-Resistance Genes in Wastewater: A Perspective from the COVID-19 Pandemic

Water, Год журнала: 2024, Номер 16(24), С. 3571 - 3571

Опубликована: Дек. 12, 2024

Antibiotic resistance is a silent global crisis intensified by the recent pandemic of coronavirus disease 2019 (COVID-19). To address this growing threat, wastewater-based surveillance (WBS) emerging as promising public health tool for monitoring antibiotic within communities. Our meta-analysis aims to reveal landscape antibiotic-resistance genes (ARGs) in wastewater during and after COVID-19 pandemic. The analysis included samples collected between 2020 2024 from five countries across three continents: Asia (China), Europe (United Kingdom Russia), North America States Canada). findings showed higher observed ARGs Russia China despite their small sample size, while USA more diverse ARGs. Distinct patterns were European American (p-value < 0.001). We identified 2483 ARGs, with multidrug-resistant (MDR) dominating most regions accounting almost 45% all detected Europe. Country-specific indicator 22 unique Russia, 3 each UK Canada, 2 specific China. Continentally, 100 Asia, 38 Europe, 18 America. These highlight regional variations ARG profiles, emphasizing urgent need region-specific strategies combat threat. Additionally, our study further supports value WBS valuable resistance.

Язык: Английский

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Influence of hospital wastewater on the development of antimicrobial resistance

Novel Research in Microbiology Journal, Год журнала: 2024, Номер 8(4), С. 2510 - 2525

Опубликована: Авг. 1, 2024

Resistance to antimicrobial agents is a developing issue that requires calls for an integrated strategy from all nations. A significant contributor resistance (AMR) wastewater vital resource bacteria which provides medium gene transfer. Stressors like high concentration of hydrogen ions, antibiotics, and minerals can start spread AMR in wastewater. Antimicrobial are present the environment varying amounts depending on class their frequent use. Evolution antibiotic-resistant (ARB) poses public health risk; especially healthcare facilities hospital's Advanced treatment technologies should be implemented effective hospital (HWW). Standardized phenotypic approaches used detect bacteria. Molecular now preferred laboratories instead as they faster more accurate detection AMR's underlying genetic mechanisms. The most common molecular assessing water include quantitative polymerase chain reaction (PCR) whole genome sequencing (WGS). Metagenomic WGS extensive genomic data taxonomic classification than PCR, it makes entire microorganisms. objectives this review were demonstrate primary mechanisms through develop antimicrobials water, investigate impact effluent ARB. Additionally, various levels guidelines preventing managing transmission discussed.

Язык: Английский

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Wastewater quality during the COVID-19 pandemic: a retrospective analysis of a Polish case study

International Journal of Environmental Science and Technology, Год журнала: 2024, Номер 22(6), С. 4125 - 4142

Опубликована: Авг. 20, 2024

Abstract The global crisis of the Coronavirus disease-2019 (COVID-19) pandemic has caused an enormous burden not only on health, social, and economic sectors but also many essential municipal services. Water distribution wastewater management have faced increasing uncertainty due to possible transmission Severe Acute Respiratory Syndrome 2 (SARS-CoV-2) via aerosols. Additionally, behavioral social changes impacted water/wastewater services need for adaptation. In this study, presence SARS-CoV-2 in influent treatment plant located Poland was analyzed November–December 2020, while other parameters important sector (e.g.: water usage quality) were monitored long-term period (2015–2020) during working days, holidays, including 2020 lockdowns. all tested samples, genetic material detected, which confirmed utility monitoring analyse COVID-19 trends local communities. Another issue elevated surfactants observed change organic matter structure raw generated Also NGS approach 16S rRNA amplicon sequencing functional diversity heterogeneity activated sludge bacterial community, especially terms nutrients removal biomass separation. Thus, it is crucial a baseline understanding how water-wastewater can serve control disease among community tools should be adopted as countermeasures new consumption patterns keeping safe effective treatment. Graphical abstract

Язык: Английский

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Antimicrobial risk assessment –aggregating aquatic chemical and resistome emissions

Water Research, Год журнала: 2024, Номер 271, С. 122929 - 122929

Опубликована: Дек. 7, 2024

Язык: Английский

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