Altered Mitochondrial Morphology and Bioenergetics in a New Yeast Model Expressing Aβ42
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(2), С. 900 - 900
Опубликована: Янв. 4, 2023
Alzheimer's
disease
(AD)
is
an
incurable,
age-related
neurological
disorder,
the
most
common
form
of
dementia.
Considering
that
AD
a
multifactorial
complex
disease,
simplified
experimental
models
are
required
for
its
analysis.
For
this
purpose,
genetically
modified
Yarrowia
lipolytica
yeast
strains
expressing
Aβ42
(the
main
biomarker
AD),
eGFP-Aβ42,
Aβ40,
and
eGFP-Aβ40
were
constructed
examined.
In
contrast
to
cells
eGFP
eGFP-Aβ40,
retaining
"normal"
mitochondrial
reticulum,
eGFP-Aβ42
possessed
disturbed
reticulum
with
fragmented
mitochondria;
was
partially
restored
by
preincubation
mitochondria-targeted
antioxidant
SkQThy.
expression
also
elevated
ROS
production
cell
death;
low
concentrations
SkQThy
mitigated
these
effects.
caused
dysfunction
as
inferred
from
loose
coupling
respiration
phosphorylation,
decreased
level
ATP
production,
enhanced
rate
hydrogen
peroxide
formation.
Therefore,
we
have
obtained
same
results
described
other
models.
Based
on
analysis
earlier
data,
suggest
fragmentation
might
be
earliest
preclinical
stage
effective
therapy
based
mitochondria-
targeted
antioxidants.
The
simple
model
can
useful
platform
rapid
screening
such
compounds.
Язык: Английский
Responsive Supramolecular Polymers for Diagnosis and Treatment
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(7), С. 4077 - 4077
Опубликована: Апрель 6, 2024
Stimuli-responsive
supramolecular
polymers
are
ordered
nanosized
materials
that
held
together
by
non-covalent
interactions
(hydrogen-bonding,
metal-ligand
coordination,
π-stacking
and,
host–guest
interactions)
and
can
reversibly
undergo
self-assembly.
Their
nature
endows
with
the
ability
to
respond
external
stimuli
(temperature,
light,
ultrasound,
electric/magnetic
field)
or
environmental
changes
pH,
redox
potential,
enzyme
activity),
making
them
attractive
candidates
for
a
variety
of
biomedical
applications.
To
date,
research
has
largely
evolved
in
development
smart
water-soluble
self-assemblies
aim
mimicking
biological
function
natural
systems.
Indeed,
there
is
wide
synthetic
biomaterials
formulated
responsiveness
control
trigger,
not
aqueous
The
design
responsive
ranges
from
use
hydrophobic
cores
(i.e.,
benzene-1,3,5-tricarboxamide)
introduction
macrocyclic
hosts
cyclodextrins).
In
this
review,
we
summarize
most
relevant
advances
achieved
stimuli-responsive
systems
used
transport
release
both
diagnosis
agents
therapeutic
drugs
order
prevent,
diagnose,
treat
human
diseases.
Язык: Английский
Radiomic features of the hippocampal based on magnetic resonance imaging in the menopausal mouse model linked to neuronal damage and cognitive deficits
Brain Imaging and Behavior,
Год журнала:
2023,
Номер
18(2), С. 368 - 377
Опубликована: Дек. 16, 2023
Estrogen
deficiency
in
the
early
postmenopausal
phase
is
associated
with
an
increased
long-term
risk
of
cognitive
decline
or
dementia.
Non-invasive
characterization
pathological
features
hallmarks
brain
women
(PMW)
could
enhance
patient
management
and
development
therapeutic
strategies.
Radiomics
a
means
to
quantify
radiographic
phenotype
diseased
tissue
via
high-throughput
extraction
mining
quantitative
from
images
acquired
modalities
such
as
CT
magnetic
resonance
imaging
(MRI).
This
study
set
out
explore
correlation
between
radiomics
based
on
MRI
hippocampus
function
PMW
mouse
model.
Ovariectomized
(OVX)
mice
were
used
PWM
models.
scans
performed
two
months
after
surgery.
The
brain's
hippocampal
region
was
manually
annotated,
radiomic
extracted
PyRadiomics.
Chemiluminescence
evaluate
peripheral
blood
estrogen
level
mice,
Morris
water
maze
test
ability
mice.
Nissl
staining
immunofluorescence
neuronal
damage
COX1
expression
sections
OVX
exhibited
marked
decline,
damage,
mitochondrial
complex
IV
subunit
COX1,
which
are
phenomena
commonly
observed
brains
AD
patients,
these
phenotypes
significantly
correlated
(p
<
0.05,
|r|>0.5),
including
Original_firstorder_Interquartile
Range,
Original_glcm_Difference
Average,
Average
Wavelet-LHH_glszm_Small
Area
Emphasis.
Meanwhile,
above
different
sham-operated
groups
0.01)
decreased
serum
levels
|r|>0.5).
initial
indicates
that
may
have
role
assessment
pathology
caused
by
using
routinely
structural
MR
images.
Язык: Английский
Non-invasive visualization of amyloid-beta deposits in Alzheimer amyloidosis mice using magnetic resonance imaging and fluorescence molecular tomography
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2021,
Номер
unknown
Опубликована: Ноя. 20, 2021
Abstract
Abnormal
cerebral
accumulation
of
amyloid-beta
peptide
(Aβ)
is
a
major
hallmark
Alzheimer’s
disease.
Non-invasive
monitoring
Aβ
deposits
enables
assessing
the
disease
burden
in
patients
and
animal
models
mimicking
aspects
human
as
well
evaluating
efficacy
Aβ-modulating
therapies.
Previous
vivo
assessments
plaque
load
mouse
amyloidosis
have
been
predominantly
based
on
two-dimensional
diffuse
fluorescence
reflectance
imaging
(2D-FRI)
two-photon
microscopy
(2PM)
using
Aβ-specific
agents.
However,
2D-FRI
lacks
depth
resolution,
whereas
2PM
restricted
by
limited
field
view
preventing
coverage
large
brain
regions.
Here,
we
utilized
magnetic
resonance
(MRI)
molecular
tomography
(FMT)
pipeline
with
curcumin
derivative
fluorescent
probe
CRANAD-2
to
achieve
full
3D
for
detecting
arcAβ
model
amyloidosis.
A
homebuilt
FMT
system
was
used
data
acquisition
combination
customized
software
platform
enabling
integration
anatomical
information
derived
from
MRI
prior
image
reconstruction.
The
results
obtained
FMT-MRI
study
were
compared
conventional
recorded
under
similar
physiological
conditions.
two
methods
yielded
comparable
time
courses
intensity
following
intravenous
injection
region
interest
comprising
brain.
resolution
inherent
allowed
separation
signal
contributions
scalp
different
regions,
indicating
preferential
tracer
cortex,
characterized
significant
deposition
arc
mice.
In
conclusion,
demonstrated
feasibility
visualizing
multimodal
method.
This
hybrid
method
provides
complementary
anatomical,
information,
thereby
detailed
characterization
status
amyloidosis,
which
also
important
putative
treatments
targeting
Aβ.
Язык: Английский