The Bovine Seminal Plasma Protein PDC-109 Possesses Pan-Antiviral Activity DOI Open Access

Hannah Sabeth Schwarzer-Sperber,

Kathrin Sutter, K. Müller

и другие.

Опубликована: Июль 5, 2022

Mammalian seminal plasma contains a multitude of bioactive components, including lipids, glucose, mineral elements, metabolites, proteins, cytokines and growth factors, with various functions during insemination fertilization. The protein PDC-109 is one the major soluble components bovine ejaculate crucially important for sperm motility, capacitation acrosome reaction. A hitherto underappreciated function its anti-microbial anti-viral activity, which may limit sexual transmission infectious diseases intercourse. We have recently discovered that inhibits membrane fusion activity influenza virus particles significantly impairs viral infections at micromolar concentrations. Here we investigated whether antiviral restricted to Influenza or if other mammalian viruses are similarly affected. focused on Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), etiological agent Coronavirus Disease 19 (COVID-19), thoroughly assessing inhibition SARS-CoV-2 Spike (S)-pseudotyped reporter particles, but also live-virus infections. Consistent our previous publications found significant inhibition, albeit accompanied by substantial cytotoxicity. Using time-of-addition experiments however, treatment regimen enable suppression without affecting cell viability. furthermore demonstrated able impair mediated VSV glycoprotein (VSVg) thus indicating broad pan-antiviral against multiple species families.

Язык: Английский

The Bovine Seminal Plasma Protein PDC-109 Possesses Pan-Antiviral Activity DOI Open Access

Hannah Sabeth Schwarzer-Sperber,

Kathrin Sutter, K. Müller

и другие.

Опубликована: Июль 5, 2022

Mammalian seminal plasma contains a multitude of bioactive components, including lipids, glucose, mineral elements, metabolites, proteins, cytokines and growth factors, with various functions during insemination fertilization. The protein PDC-109 is one the major soluble components bovine ejaculate crucially important for sperm motility, capacitation acrosome reaction. A hitherto underappreciated function its anti-microbial anti-viral activity, which may limit sexual transmission infectious diseases intercourse. We have recently discovered that inhibits membrane fusion activity influenza virus particles significantly impairs viral infections at micromolar concentrations. Here we investigated whether antiviral restricted to Influenza or if other mammalian viruses are similarly affected. focused on Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), etiological agent Coronavirus Disease 19 (COVID-19), thoroughly assessing inhibition SARS-CoV-2 Spike (S)-pseudotyped reporter particles, but also live-virus infections. Consistent our previous publications found significant inhibition, albeit accompanied by substantial cytotoxicity. Using time-of-addition experiments however, treatment regimen enable suppression without affecting cell viability. furthermore demonstrated able impair mediated VSV glycoprotein (VSVg) thus indicating broad pan-antiviral against multiple species families.

Язык: Английский

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