Kidney-Specific Membrane-Bound Serine Proteases CAP1/Prss8 and CAP3/St14 Affect ENaC Subunit Abundances but Not Its Activity DOI Open Access
Elodie Ehret,

Sévan Stroh,

Muriel Auberson

и другие.

Опубликована: Авг. 14, 2023

The serine proteases CAP1/Prss8 (prostasin) and CAP3/St14 (matriptase) are identified as ENaC channel-activating in vitro highly suggesting that they required for proteolytic activation of vivo. present study tested whether CAP3 is relevant renal affects ENaC-mediated Na+ absorption following Na+-deprivation conditions. knockout mice exhibit significant decrease CAP1 protein expression with altered subunit decreased pNCC abundances, but overall maintain sodium balance. RNAscope-based analyses reveal co-expression alpha distal tubules the cortex from wildtype mice. Double CAP1/CAP3-deficiency maintained K+ balance on Na+-deprived diet, restored abundances showed reduced NCC activity under Na+-deprivation. Overall, our data clearly show not direct ENaC, its abundances. Our a complex regulation by these level rather than activation.

Язык: Английский

GPI-anchored serine proteases: essential roles in development, homeostasis, and disease DOI Creative Commons

Joseph G. Lundgren,

Michael G. Flynn,

Karin List

и другие.

Biological Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 17, 2025

Abstract The glycosylphosphatidylinositol (GPI)-anchored serine proteases, prostasin and testisin, have essential roles in diverse physiological functions including development, reproduction, homeostasis barrier function of epithelia, angiogenesis, coagulation, fibrinolysis. Important pathological conditions such as cancer, kidney disease cardiovascular also been reported. In this review, we summarize current knowledge the cellular vivo testisin physiology pathophysiology explore underlying molecular mechanisms. We discuss how new insights their role cancer may facilitate translation into clinical settings future.

Язык: Английский

Процитировано

0

Kidney-Specific Membrane-Bound Serine Proteases CAP1/Prss8 and CAP3/St14 Affect ENaC Subunit Abundances but Not Its Activity DOI Open Access
Elodie Ehret,

Sévan Stroh,

Muriel Auberson

и другие.

Опубликована: Авг. 14, 2023

The serine proteases CAP1/Prss8 (prostasin) and CAP3/St14 (matriptase) are identified as ENaC channel-activating in vitro highly suggesting that they required for proteolytic activation of vivo. present study tested whether CAP3 is relevant renal affects ENaC-mediated Na+ absorption following Na+-deprivation conditions. knockout mice exhibit significant decrease CAP1 protein expression with altered subunit decreased pNCC abundances, but overall maintain sodium balance. RNAscope-based analyses reveal co-expression alpha distal tubules the cortex from wildtype mice. Double CAP1/CAP3-deficiency maintained K+ balance on Na+-deprived diet, restored abundances showed reduced NCC activity under Na+-deprivation. Overall, our data clearly show not direct ENaC, its abundances. Our a complex regulation by these level rather than activation.

Язык: Английский

Процитировано

2