Опубликована: Сен. 20, 2023

Alopecurus myosuroides (blackgrass) is a problematic weed of Western European winter wheat and its success largely due to widespread multiple-herbicide resistance. Previous analysis F2 seed families derived from two distinct blackgrass populations exhibiting equivalent non-target site resistance (NTSR) phenotypes shows polygenic evolves standing genetic variation. Using CIDER-seq pipeline we show that herbicide resistant (HR) sensitive (HS) F3 plants these as well the parent they were carry extra-chromosomal circular DNA (eccDNA). We identify similarities differences in coding structures within between populations. Although numbers size detected eccDNAs varied populations, comparisons HR HS identified shared unique content, predicted genes, functional protein domains. These include genes related detoxification such Cytochrome P450s, ATP-binding cassette transporters glutathione transferases including AmGSTF1. eccDNA content was mapped reference genome, revealing genomic regions at distal end chromosome 5 near center 1 7 with high number gene density. Mapping 15 known herbicide-resistant QTL showed sequences match 12 four QLT matching one region only contained sequences. findings establish like other pernicious weeds, has contain homologs chromosomal may contribute heterogeneity evolutionary innovation rapidly adapt abiotic stresses treatment.

Язык: Английский