Uncovering the Host Range–Lifestyle Relationship in the Endophytic and Anthracnose Pathogenic Genus Colletotrichum
Microorganisms,
Год журнала:
2025,
Номер
13(2), С. 428 - 428
Опубликована: Фев. 16, 2025
Colletotrichum
includes
agriculturally
and
scientifically
important
pathogens
that
infect
numerous
plants.
They
can
also
adopt
an
endophytic
lifestyle,
refraining
from
causing
disease
and/or
even
promoting
plant
growth
when
inoculated
on
a
non-susceptible
host.
In
this
manner,
the
host
range
of
fungus
shift,
depending
whether
it
exhibits
or
pathogenic
lifestyles.
Some
fungi,
such
as
tofieldiae,
shift
between
pathogenicity
endophytism
within
same
environmental
conditions.
Here,
we
aim
to
disentangle
relationship
lifestyle
in
Colletotrichum.
Specifically,
demonstrate
is
dependent
colonized
many
fungi.
We
discuss
ways
which
species
may
act
endophytically
alternative
hosts,
how
comparative
genomics
has
uncovered
candidate
molecules
(namely
effectors,
CAZymes,
secondary
metabolites)
underlying
fungal
merits
using
fungi
alongside
research,
facilitates
use
reverse
genetics
uncover
molecular
determinants
lifestyle.
particular,
reference
Arabidopsis
thaliana-Colletotrichum
tofieldiae
study
system
model
for
elucidating
dual
roles
plant-fungus
interactions,
both
pathogenic,
through
integrative
omics
approaches
genetics.
This
because
C.
contains
closely
related
endophytes,
making
ideal
identifying
approach
could
identify
key
targets
effective
pathogen
management
agriculture.
Lastly,
propose
occupies
different
than
will
enhance
our
understanding
globally
significant
genus
lay
groundwork
future
research
examining
plant-associated
Язык: Английский
Comparative Genomic Analysis of <em>Colletotrichum lini </em>Strains with Different Virulence on Flax
Опубликована: Дек. 13, 2023
Colletotrichum
lini
is
a
flax
fungal
pathogen,
comprising
differently
virulent
strains.
In
this
work,
we
sequenced
the
genomes
of
three
C.
strains
different
virulence:
high
#390-1,
medium
#757,
and
low
#771.
We
obtained
more
than
100×
genome
coverage
with
Oxford
Nanopore
Technologies
reads
50×
Illumina
data.
To
construct
high-quality
genomes,
several
assembly
strategies
were
tested.
The
final
assemblies
using
Canu
–
Racon
×2
Medaka
Polca
scheme
54.0-55.3
Mb
in
length,
contained
26-32
contigs,
had
an
N50
>
5
BUSCO
completeness
96%.
Comparative
analysis
constructed
showed
similarity
between
mitochondrial
nuclear
genomes.
However,
rearrangement
event
loss
0.9
contig
revealed.
candidate
effectors
predicted
sets
proteins
received
after
annotation.
sequences
extend
knowledge
on
genetic
diversity
species
are
basis
for
establishing
molecular
mechanisms
pathogenicity
pathogen
effective
control
anthracnose.
Язык: Английский
Complete Annotated Genome Assembly of Flax Pathogen Colletotrichum lini
Journal of Fungi,
Год журнала:
2024,
Номер
10(9), С. 605 - 605
Опубликована: Авг. 26, 2024
is
a
fungal
pathogen
of
flax
that
can
cause
significant
yield
and
quality
losses.
In
this
work,
we
obtained
the
first
complete
annotated
genome
assembly
highly
virulent
Язык: Английский
Comparative Genomic Analyses of Colletotrichum lindemuthianum Pathotypes with Different Virulence Levels and Lifestyles
Ma. Irene Morelos-Martínez,
Horacio Cano-Camacho,
Karla Morelia Díaz-Tapia
и другие.
Journal of Fungi,
Год журнала:
2024,
Номер
10(9), С. 651 - 651
Опубликована: Сен. 13, 2024
is
the
most
frequent
pathogenic
fungus
of
common
bean
Язык: Английский
Nanopore Data-Driven T2T Genome Assemblies of Colletotrichum lini Strains
Journal of Fungi,
Год журнала:
2024,
Номер
10(12), С. 874 - 874
Опубликована: Дек. 16, 2024
Colletotrichum
lini
is
a
pathogenic
fungus
that
infects
flax
and
causes
significant
yield
losses.
In
this
study,
we
assembled
the
genomes
of
four
highly
virulent
C.
strains
using
Oxford
Nanopore
Technologies
(ONT,
R10.4.1
flow
cells)
Illumina
platforms.
The
performance
two
tools
developed
for
telomere-to-telomere
(T2T)
genome
assembly
was
compared:
Verkko
Hifiasm.
Prior
to
assembly,
ONT
reads
were
corrected
HERRO
algorithm.
generated
assemblies
high
completeness
but
low
contiguity,
while
Hifiasm
allowed
generation
T2T
assemblies.
Despite
significantly
different
coverage
with
data
(25–100×),
equal
contiguity
obtained:
53.6–54.7
Mb,
ten
core
chromosomes,
or
three
accessory
chromosomes.
A
comparative
analysis
polishing
showed
at
certain
(≥35×),
additional
did
not
improve
its
accuracy,
even
data.
An
structures
revealed
similarity
between
Thus,
our
approach
enabled
assembling
only
from
obtained
cells
may
be
promising
other
fungal
genera.
These
will
allow
accurate
identification
strain-specific
differences
chromosome
level
aid
in
development
effective
strategies
protect
anthracnose.
Язык: Английский