Isolation and Characterization of Lytic Phages Infecting Clinical Klebsiella pneumoniae from Tunisia DOI Creative Commons
Donia Mourali, Rahma Kazdaghli, Marwa Gara-Ali

и другие.

Antibiotics, Год журнала: 2024, Номер 13(12), С. 1154 - 1154

Опубликована: Дек. 2, 2024

Background: Klebsiella pneumoniae is an opportunistic pathogen that causes a wide range of infections worldwide. The emergence and spread multidrug-resistant clones requires the implementation novel therapeutics, phages are promising approach. Results: In this study, two phages, KpTDp1 KpTDp2, were isolated from wastewater samples in Tunisia. These had narrow host specifically targeted hypervirulent K2 K28 capsular types K. pneumoniae. Both have double-stranded linear DNA genomes 49,311 49,084 bp, respectively. Comparative genomic phylogenetic analyses placed phage KpTDp2 genus Webervirus, while showed some homology with members Jedunavirus, although its placement new undescribed may be reconsidered. replication efficiency lytic ability these combined their high stability at temperatures up to 70 °C pH values ranging 3.5 8.2, highlight potential as good candidates for control Methods: Phage isolation, titration multiplicity infection performed. was tested different temperatures. Genomic characterization done by genome sequencing, annotation analysis. Conclusions: lyse one most virulent serotypes pneumoniae, well activities temperature variations, make antibacterial control.

Язык: Английский

The potential use of bacteriophages as antibacterial agents against Klebsiella pneumoniae DOI Creative Commons

Omid Gholizadeh,

Hadi Esmaeili Gouvarchin Ghaleh,

Mahdi Tat

и другие.

Virology Journal, Год журнала: 2024, Номер 21(1)

Опубликована: Авг. 19, 2024

One of the most common bacteria that cause nosocomial infections is Klebsiella pneumonia (K. pneumoniae), especially in patients who are very sick and admitted to intensive care unit (ICU). The frequency multi-drug-resistant pneumoniae (MDRKP) has dramatically increased worldwide recent decades, posing an urgent threat public health. Western world's bacteriophage (phage) studies have been revitalized due increasing reports antimicrobial resistance restricted development discovery new antibiotics. These factors also spurred innovation other scientific domains. primary agent phage treatment obligately lytic organism (called bacteriophage) kills corresponding bacterial host while sparing human cells lessening broader effects antibiotic usage on commensal bacteria. Phage developing quickly, leading many clinical instances life-saving medicinal use. In addition, a few immunological adverse consequences addition its usefulness. Since K. made treating multidrug-resistant (MDR) challenging, therapy (PT) emerged as novel therapeutic strategy. effectiveness phages investigated biofilms animal infection models. Compared with antibiotics, PT exhibits numerous advantages, including particular lysis spectrum, co-evolution avoid emergence resistance, higher abundance diversity resources than found Moreover, eliminated absence bacterium, which makes them only self-regulates at sites infection. Therefore, it essential pay attention role these infections. This study summarizes state knowledge spp. provides outlook phage-based treatments target trials.

Язык: Английский

Процитировано

10
Potential of a New Bacteriophage to Control <em>Klebsiella pneumoniae</em>: Preliminary Studies to Control Urinary Tract Infections DOI Open Access
João Duarte,

Carolina Máximo,

Pedro Costa

и другие.

Опубликована: Янв. 8, 2024

Urinary tract infections (UTIs) caused by Klebsiella pneumoniae can lead to severe clinical complications and even death. An alternative treatment option for infected patients is using bacteriophages (phages). In the present study, we isolated phage KP-1 from sewage water K. as a host. Whole genome analysis indicated that was double-stranded linear 176,096-bp long DNA molecule with 41.8% GC content did not contain virulence or antibiotic resistance genes. The inactivation potential of assessed in broth at an MOI 1 10, maximum 4.9 5.4 log CFU/mL observed after 9 h, respectively. 10 efficacy also urine evaluate phage’s performance acidic environment. A 3.8 h. results indicate could be used control UTIs pneumoniae; however, further studies cocktails broader spectrum activity phages micro- nanocarriers avoid negative effect low pH on viability, are necessary.

Язык: Английский

Процитировано

6
Isolation, Characterization, and Anti-Biofilm Activity of a Novel Kaypoctavirus Against K24 Capsular Type, Multidrug-Resistant Klebsiella pneumoniae Clinical Isolates DOI Creative Commons
Phitchayapak Wintachai,

Joanne M. Santini,

Renuka Thonguppatham

и другие.

Antibiotics, Год журнала: 2025, Номер 14(2), С. 157 - 157

Опубликована: Фев. 5, 2025

Background/Objectives: The significant outbreak of multidrug-resistant Klebsiella pneumoniae has emerged as a primary global concern associated with high morbidity and mortality rates. Certain strains K. are highly resistant to most antibiotics available in clinical practice, exacerbating the challenge bacterial infections. Methods: Phage vB_KpnP_PW7 (vKPPW7) was isolated characterized. Its morphology, stability, adsorption rate, one-step growth curve, lytic activity, whole-genome sequence analysis, antibacterial antibiofilm activities were evaluated. Results: virulent phage 73,658 bp linear dsDNA genome classified new species genus Kaypoctavirus, subfamily Enquatrovirinae, family Schitoviridae. vKPPW7 short latent period, large burst size. showed activity against 18 isolates K24 capsular type but unable lyse whose not K24. Additionally, demonstrated strong stability across various temperatures pH values. exhibited scanning electron microscopy (SEM) confirmed its ability MDR type. Furthermore, effectively removed preformed biofilm prevented formation, resulting reduced biomass viability compared controls. architecture phage-treated biofilms under SEM. Conclusions: These findings suggest that holds promise for development therapeutic or biocontrol agent.

Язык: Английский

Процитировано

0
The Potential of Phage Treatment to Inactivate Planktonic and Biofilm-Forming Pseudomonas aeruginosa DOI Creative Commons

Inês Martinho,

Márcia Braz, João Duarte

и другие.

Microorganisms, Год журнала: 2024, Номер 12(9), С. 1795 - 1795

Опубликована: Авг. 29, 2024

Pseudomonas aeruginosa is a common cause of hospital-acquired infections and exhibits strong resistance to antibiotics. An alternative treatment option for bacterial the use bacteriophages (or phages). In this study, two distinct phages, VB_PaD_phPA-G (phPA-G) VB_PaN_phPA-Intesti (phPA-Intesti), were used as single suspensions or in phage cocktail inactivate planktonic cells biofilms P. aeruginosa. Preliminary experiments culture medium showed that phPA-Intesti (reductions 4.5–4.9 log CFU/mL) outperformed phPA-G 0.6–2.6 (reduction 4.2 CFU/mL). Phage caused maximum reduction 5.5 CFU/cm2 biofilm urine after 4 h incubation. The combination ciprofloxacin did not improve efficacy inactivation nor reduce development resistant mutants. However, bacteria was lower combined with antibiotic compared alone. This lacks known toxins, virulence, resistance, integrase genes. Overall, results suggest could be potential approach control urinary tract (UTIs), namely those by biofilm-producing multidrug-resistant strains

Язык: Английский

Процитировано

1
Isolation and Characterization of Infection of Four New Bacteriophages Infecting a Vibrio parahaemolyticus Strain DOI Creative Commons
João Duarte,

David Trindade,

Vanessa Oliveira

и другие.

Antibiotics, Год журнала: 2024, Номер 13(11), С. 1086 - 1086

Опубликована: Ноя. 14, 2024

Bacteria from genus Vibrio continue to be one of the most common threats aquaculture sustainability.

Язык: Английский

Процитировано

1
Isolation and Characterization of Lytic Phages Infecting Clinical Klebsiella pneumoniae from Tunisia DOI Creative Commons
Donia Mourali, Rahma Kazdaghli, Marwa Gara-Ali

и другие.

Antibiotics, Год журнала: 2024, Номер 13(12), С. 1154 - 1154

Опубликована: Дек. 2, 2024

Background: Klebsiella pneumoniae is an opportunistic pathogen that causes a wide range of infections worldwide. The emergence and spread multidrug-resistant clones requires the implementation novel therapeutics, phages are promising approach. Results: In this study, two phages, KpTDp1 KpTDp2, were isolated from wastewater samples in Tunisia. These had narrow host specifically targeted hypervirulent K2 K28 capsular types K. pneumoniae. Both have double-stranded linear DNA genomes 49,311 49,084 bp, respectively. Comparative genomic phylogenetic analyses placed phage KpTDp2 genus Webervirus, while showed some homology with members Jedunavirus, although its placement new undescribed may be reconsidered. replication efficiency lytic ability these combined their high stability at temperatures up to 70 °C pH values ranging 3.5 8.2, highlight potential as good candidates for control Methods: Phage isolation, titration multiplicity infection performed. was tested different temperatures. Genomic characterization done by genome sequencing, annotation analysis. Conclusions: lyse one most virulent serotypes pneumoniae, well activities temperature variations, make antibacterial control.

Язык: Английский

Процитировано

0