Identification of m6A methyltransferase-related WTAP and ZC3H13 predicts immune infiltrates in glioblastoma
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Фев. 5, 2025
Glioblastoma
(GBM)
is
a
prevalent
and
highly
fatal
primary
malignant
brain
tumor.
N6-methyladenosine
(m6A)
modification
plays
critical
role
in
the
development
of
WTAP
ZC3H13
have
been
identified
across
various
species.
Immune
contexture,
which
includes
tumor
microenvironment
(TME),
significant
cancer
progression
treatment.
This
study
aimed
to
explore
potential
impact
between
on
immunological
characteristics
GBM.
We
utilized
data
from
TCGA-GBM,
GEO
CGGA
datasets
obtain
platform
probe
data.
Patients
with
GBM
were
stratified
into
two
clusters
based
expression
using
consensus
clustering
approach.
infiltration
within
was
assessed
ESTIMATE,
CIBERSORT
ssGSEA
methodologies.
Functional
disparities
determined
through
gene
set
enrichment
analysis
(GSEA).
Tumor
mutation
burden
(TMB)
immune
checkpoint
inhibitors
(ICIs)
also
analyzed.
Co-expression
network
(WGCNA)
used
identify
genes
associated
WTAP/ZC3H13
immunity.
Validation
performed
datasets.
Our
revealed
that
cluster1
exhibited
higher
but
lower
compared
cluster2.
Cluster1
showed
levels
TMB
WGCNA
15
closely
scores,
notably
CTLA4,
CD27,
ICOS,
LAG3.
results
suggested
influence
contexture
GBM,
providing
new
insights
immunity
Язык: Английский
RNA N6-Methyladenosine Modification in DNA Damage Response and Cancer Radiotherapy
Опубликована: Янв. 12, 2024
N6-methyladenosine
(M6A)
modification
is
the
most
abundant
internal
chemical
on
RNA
molecules
in
eukaryotes
and
can
affect
mRNA
metabolism
regulate
transcription,
nuclear
export,
splicing,
degradation,
translation,
ets,
has
a
significant
impact
various
aspects
of
physiology
or
pathobiology.
Radiotherapy
common
measure
tumor
treatment,
variety
cellular
intrinsic
mechanisms
responses
cells
to
ionizing
radiation
(IR)
effectiveness
cancer
radiotherapy.
In
this
review,
we
summarize
discuss
recent
advances
about
roles
M6A
methylation
radiation-induced
DNA
damage
response,
outcomes
Insights
into
biology
may
facilitate
improvement
therapeutic
strategies
for
radiotherapy
as
well
radioprotection
normal
tissues.
Язык: Английский