Small Extracellular Vesicles Derived from Cord Blood Plasma and Placental Mesenchymal Stem Cells Attenuate Acute Lung Injury Induced by Lipopolysaccharide (LPS) DOI Open Access
Ranga P. Thiruvenkataramani, Amal Abdul‐Hafez,

Tulasi Kesaraju

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 26(1), С. 75 - 75

Опубликована: Дек. 25, 2024

Sepsis is a risk factor associated with increasing neonatal morbidity and mortality, acute lung injury, chronic disease. While stem cell therapy has shown promise in alleviating its effects are primarily exerted through paracrine mechanisms rather than local engraftment. Accumulating evidence suggests that these mediated by mesenchymal (MSC)-derived small extracellular vesicles (sEVs), which play critical role immune system modulation tissue regeneration. sEVs contain diverse cargo of mRNA, miRNA, proteins, contributing to their therapeutic potential. We hypothesize derived from three distinct sources, cord blood plasma (CBP), Wharton jelly (WJ), placental (PL) MSCs, may prevent the cytotoxicity induced E. coli lipopolysaccharide (LPS) alveolar epithelial cells. Objective: To determine CBP-, WJ-, PL-MSCs-derived on viability, apoptosis, proinflammatory cytokine production cells monocytes following LPS treatment. were collected conditioned media PL-MSCs, WJ-MSCs, CBP using 50 nm membrane filters. characterized based nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), Western blotting techniques. The protein concentration isolated was used standardize treatment doses. A549 monocyte THP-1 cultured exposed presence or absence for 72 h. Cell viability measured CellTiter-Glo 2.0 chemiluminescence-based assay. For analysis, pre-incubated 24 h without PL- CBP-sEVs, followed exposure control conditions an additional collected, interleukin-6 (IL-6) interleukin-8 (IL-8) levels quantified ELISA. significantly reduced both CB- WJ-sEVs increased compared controls. Cells treated PL-sEVs showed but did not reach statistical significance. LPS-treated significant increase apoptosis elevated pro-inflammatory cytokines IL-6 IL-8. All types (CBP-, PL-sEVs) LPS-induced release. Interestingly, while decreased IL-8, CBP- led IL-8 respective PL-, WJ-derived demonstrated protective against injury monocytes, as evidenced These findings suggest placenta-derived have potential modulate response, mitigate inflammation, end-organ damage sepsis.

Язык: Английский

Use of Extracellular Vesicles in the Therapy of Neonatal Diseases: Current State and Problems of Translation to the Clinic DOI Open Access
Кirill Goryunov, Mikhail Ivanov, A.Yu. Kulikov

и другие.

Опубликована: Янв. 16, 2024

Neonatal disorders, particularly those resulting from prematurity, pose a major challenge to health care and have significant impact on infant mortality long-term child health. The limitations of current therapeutic strategies emphasize the need for innovative treatments. New cell-free technologies utilizing extracellular vesicles (EVs) offer compelling opportunity neonatal therapy by harnessing inherent regenerative capabilities EVs. These nanoscale particles, secreted variety organisms including animals, bacteria, fungi plants, contain repertoire bioactive molecules with potential. This review aims provide comprehensive assessment effects mechanistic insights EVs stem cells, biological fluids non-animal sources, focus common conditions such as hypoxic-ischemic encephalopathy, respiratory distress syndrome, bronchopulmonary dysplasia necrotizing enterocolitis. summarizes evidence potential EVs, analyzes their mechanisms action, discusses challenges associated implementation EV-based therapies in clinical practice.

Язык: Английский

Процитировано

2
Small Extracellular Vesicles Derived from Cord Blood Plasma and Placental Mesenchymal Stem Cells Attenuate Acute Lung Injury Induced by Lipopolysaccharide (LPS) DOI Open Access
Ranga P. Thiruvenkataramani, Amal Abdul‐Hafez,

Tulasi Kesaraju

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 26(1), С. 75 - 75

Опубликована: Дек. 25, 2024

Sepsis is a risk factor associated with increasing neonatal morbidity and mortality, acute lung injury, chronic disease. While stem cell therapy has shown promise in alleviating its effects are primarily exerted through paracrine mechanisms rather than local engraftment. Accumulating evidence suggests that these mediated by mesenchymal (MSC)-derived small extracellular vesicles (sEVs), which play critical role immune system modulation tissue regeneration. sEVs contain diverse cargo of mRNA, miRNA, proteins, contributing to their therapeutic potential. We hypothesize derived from three distinct sources, cord blood plasma (CBP), Wharton jelly (WJ), placental (PL) MSCs, may prevent the cytotoxicity induced E. coli lipopolysaccharide (LPS) alveolar epithelial cells. Objective: To determine CBP-, WJ-, PL-MSCs-derived on viability, apoptosis, proinflammatory cytokine production cells monocytes following LPS treatment. were collected conditioned media PL-MSCs, WJ-MSCs, CBP using 50 nm membrane filters. characterized based nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), Western blotting techniques. The protein concentration isolated was used standardize treatment doses. A549 monocyte THP-1 cultured exposed presence or absence for 72 h. Cell viability measured CellTiter-Glo 2.0 chemiluminescence-based assay. For analysis, pre-incubated 24 h without PL- CBP-sEVs, followed exposure control conditions an additional collected, interleukin-6 (IL-6) interleukin-8 (IL-8) levels quantified ELISA. significantly reduced both CB- WJ-sEVs increased compared controls. Cells treated PL-sEVs showed but did not reach statistical significance. LPS-treated significant increase apoptosis elevated pro-inflammatory cytokines IL-6 IL-8. All types (CBP-, PL-sEVs) LPS-induced release. Interestingly, while decreased IL-8, CBP- led IL-8 respective PL-, WJ-derived demonstrated protective against injury monocytes, as evidenced These findings suggest placenta-derived have potential modulate response, mitigate inflammation, end-organ damage sepsis.

Язык: Английский

Процитировано

2