Pulsed Electromagnetic Fields (PEMFs) Trigger Cell Death and Senescence in Cancer Cells
Pavlos Pantelis,
Giorgos Theocharous,
Dimitris Veroutis
и другие.
Опубликована: Янв. 25, 2024
The
currently
available
anti-cancer
therapies,
such
as
gamma-radiation
and
chemotherapeutic
agents,
induce
cell
death
cellular
senescence
not
only
in
cancer
cells
but
also
the
adjacent
normal
tissue.
New
anti-tumor
approaches
focus
on
limiting
side
effects
cells.
In
this
frame,
potential
properties
of
Pulsed
Electromagnetic
Fields
(PEMFs)
through
irradiation
breast
epithelial
(MCF-7
MDA-MB-231)
fibroblasts
(FF95)
were
investigated.
PEMFs
had
a
frequency
8
Hz,
full-square
wave
type,
magnetic
flux
density
0.011
T
applied
twice
daily
for
5
days.
Data
collected
showcases
that
PEMF
application
decreases
proliferation
rate
viability
while
having
opposite
effect
fibroblasts.
Moreover,
induced
without
any
non-cancerous
These
findings
suggest
novel,
non-invasive
strategy
combination
with
senolytic
drugs,
to
eliminate
both
remaining
senescent
simultaneously
avoiding
current
treatments.
Язык: Английский
Radiofrequency Electromagnetic and Pulsed Magnetic Fields Protected the Kidney Against Lipopolysaccharide-Induced Acute Systemic Inflammation, Oxidative Stress, and Apoptosis by Regulating the IL-6/HIF1α/eNOS and Bcl2/Bax/Cas-9 Pathways
Medicina,
Год журнала:
2025,
Номер
61(2), С. 238 - 238
Опубликована: Янв. 29, 2025
Background/Objectives:
Sepsis-associated
acute
kidney
injury
caused
by
lipopolysaccharide
(LPS)
is
related
to
hypoxia,
amplification
of
the
inflammatory
response,
oxidative
stress,
mitochondrial
dysfunction,
and
apoptosis.
This
study
aims
explore
protective
effects
a
radiofrequency
electromagnetic
field
(RF-EMF)
pulsed
magnetic
(PMF)
on
in
rats.
Materials
methods:
Forty
female
Wistar
albino
rats
were
randomly
divided
into
five
groups
(each
containing
eight
rats):
control,
LPS,
RF-EMF,
PMF,
RF-EMF
+
PMF
groups.
Six
hours
after
LPS
application,
blood
tissues
removed
for
histopathological,
immunohistochemical,
biochemical,
genetic
analysis.
Results:
Histopathological
findings,
caspase-3,
inducible
nitric
oxide
synthase
tumor
necrosis
factor-alpha
immunoexpressions,
total
oxidant
status
stress
index
levels,
interleukin-6,
hypoxia-inducible
factor
alpha,
Bcl-2-associated
X
protein,
caspase
9
gene
expression
tissue
urine
nitrogen
creatinine
levels
increased,
whereas
endothelial
B-cell
lymphoma
2
decreased
Both
reversed
all
these
findings
recovered
renal
tissues.
Conclusions:
Noninvasive,
nontoxic,
low-cost
both
single
combined,
have
been
demonstrated
renoprotective
anti-inflammatory,
antioxidant,
antiapoptotic
effects.
Язык: Английский
Pulsed Electromagnetic Field Therapy Alters the Genomic Profile of Bladder Cancer Cell Line HT-1197
Journal of Personalized Medicine,
Год журнала:
2025,
Номер
15(4), С. 143 - 143
Опубликована: Апрель 4, 2025
Background/Objectives:
Pulsed
electromagnetic
field
(PEMF)
therapy
involves
the
use
of
magnetic
waveform
energy
for
targeted
treatment
delivery.
This
technique
has
shown
promising
results
in
various
cancers.
Currently,
bladder
cancer
is
highly
invasive,
involving
intravesical
chemotherapy
or
radical
cystectomy.
The
potential
therapeutic
effects
PEMF
on
are
a
relatively
new
and
understudied
area;
therefore,
goal
this
investigation
was
to
gain
mechanistic
insight
by
examining
cell
line
vitro.
Methods:
Cells
from
HT-1197
were
cultured
incubated
with
(treatment
group)
without
(control
one
hour
each
day
five
days.
Cell
counts
compared
using
Incucyte®
data
determine
proliferation
rates.
At
days
1
5,
total
RNA
isolated
cells,
following
quantity
quality
checks,
gene
expression
between
two
groups.
Proliferation
rates
prior
published
HT-1376.
Results:
cells
treated
had
slower
controls
(p
<
0.05),
but
HT-1376
did
not
>
0.05).
Principal
component
analysis
showed
complete
separation
untreated
accounting
76%
variation
Expression
numerous
genes
cancer-related
pathways
altered
relative
controls.
Conclusions:
Bladder
corresponding
changes
expression.
Several
cancer-relevant
differentially
regulated
treatment.
conclusions
limited
lack
control
healthy
urothelial
experiments.
Despite
shortcoming,
our
suggest
that
may
be
avenue
further
research
cancer.
Язык: Английский
Unveiling the Performance of Co-Assembled Hybrid Nanocarriers: Moving towards the Formation of a Multifunctional Lipid/Random Copolymer Nanoplatform
Pharmaceutics,
Год журнала:
2024,
Номер
16(9), С. 1204 - 1204
Опубликована: Сен. 13, 2024
Despite
the
appealing
properties
of
random
copolymers,
use
these
biomaterials
in
association
with
phospholipids
is
still
limited,
as
several
aspects
their
performance
have
not
been
investigated.
The
aim
this
work
formulation
lipid/random
copolymer
platforms
and
comprehensive
study
features
by
multiple
advanced
characterization
techniques.
Both
are
amphiphilic,
including
two
(1,2-dioctadecanoyl-sn-glycero-3-phosphocholine
(DSPC),
1,2-dioleoyl-sn-glycero-3-phosphocholine
(DOPC))
a
statistical
oligo
(ethylene
glycol)
methyl
ether
methacrylate
(OEGMA)
2-(diisopropylamino)
ethyl
(DIPAEMA).
We
examined
design
parameters,
lipid
composition,
%
comonomer
ratio,
lipid-to-polymer
ratio
that
could
be
critical
for
behavior.
structures
were
also
probed
different
conditions.
To
best
authors'
knowledge,
first
time
P(OEGMA-co-DIPAEMA)/lipid
hybrid
colloidal
dispersions
investigated
from
membrane
mechanics,
biophysical,
morphological
perspective.
Among
other
architecture
hydrophilic
to
hydrophobic
balance
deemed
fundamental
parameters
biomaterial
co-assembly,
having
an
impact
on
membrane's
fluidity,
morphology,
thermodynamics.
Exploiting
unique
characteristics,
most
promising
candidates
utilized
methotrexate
(MTX)
loading
explore
encapsulation
capability
potential
antitumor
efficacy
vitro
various
cell
lines.
Язык: Английский