Stamp-Imprinted Polymer EIS Biosensor for Amyloid-Beta Detection: A Novel Approach Towards Alzheimer’s Screening

Biosensors, Год журнала: 2025, Номер 15(4), С. 228 - 228

Опубликована: Апрель 3, 2025

Surface-imprinted polymers (SIPs) represent an exciting and cost-effective alternative to antibodies for electrochemical impedance spectroscopy (EIS)-based biosensing. They can be produced using simple printing techniques have shown high efficacy in detecting large biomolecules microorganisms. Stamp imprinting, a novel SIP method, creates the target analyte’s imprint soft lithography mask of analyte matrix, thereby reducing material complexities eliminating need cross-linking, which makes process more scalable than conventional SIPs. In this work, we demonstrate stamp-imprinted EIS biosensor biocompatible polymer, polycaprolactone (PCL), quantifying amyloid beta-42 (Aβ-42), small peptide involved pathophysiology Alzheimer’s disease. The evaluated SIP-EIS biosensors showed detection limit close 10 fg/mL, range covering physiologically relevant concentration blood serum (from fg/mL μg/mL). device sensitivity, is found comparable antibody-based devices, demonstrates potential as diagnostic approaches. We also evaluate viability analyzing these proteins complex media, notably presence albumin proteins, cause biofouling non-specific interactions. combination selectivity, ease fabrication particularly suited portable point-of-care applications.

Язык: Английский

Is this the Simplest Possible Biosensor? A Surface Imprinted Polymer EIS Sensor for Detecting Alpha-synuclein, a Parkinson’s Disease Biomarker

Опубликована: Янв. 25, 2024

Parkinson’s Disease (PD) is a debilitating neurodegenerative disease, causing loss of motor function, and in some instances, cognitive decline dementia, those affected. The quality life can be improved, disease progression delayed through early interventions, however, current methods confirming PD diagnosis are extremely invasive. This prevents their use as screening tool for the onset stages PD. We propose surface imprinted polymer (SIP) electroimpedance spectroscopy (EIS) biosensor detecting α-Synuclein (αSyn) its aggregates, biomarker that appears saliva blood during brain barrier degrades. stamp fabricated by low temperature melt stamping polycaprolactone (PCL) on interdigitated EIS electrodes. result low-cost, small footprint highly suitable non-invasive monitoring biomarker. sensors were tested with αSyn dilutions deionized water, constant ionic concentration matrix solutions decreasing concentrations to remove background effects concentration. device response confirmed specificity these devices target protein monomeric αSyn. sensor limit detection was measured 5 pg/L linear range – µg/L. covers physiological makes this promising method quantifying monomers patients future. SIP regenerated, reused demonstrate capability repeat sensing potential continuous

Язык: Английский