Early Stages of Ex Vivo Collagen Glycation Disrupt the Cellular Interaction and Its Remodelling by Mesenchymal Stem Cells—Morphological and Biochemical Evidence

Опубликована: Фев. 26, 2024

Mesenchymal stem cells (MSCs), whose main function is tissue repair, use collagen to restore the structural integrity of damaged tissue, maintaining its organization through concomitant remodelling. The non-enzymatic glycation likely compromise communication with MSC, which indeed underlies various pathological conditions such as late complications diabetes and aging. However, data on effect more early stages MSC interaction are lacking. This study focused fate in vitro glycated rat tail (RTC) contact MSCs after pre-exposure glucose for 1 or 5 days. Using human adipose tissue-derived (ADMSCs), we showed that their significantly altered, manifested morphologically by reduced cell spreading, less formation focal adhesions, weaker development actin cytoskeleton, further confirmed ImageJ morphometric analysis. suggests a poorer recognition integrin receptors, possibly due steric hindrance binding sites. These morphological events were also accompanied greatly fibril-like reorganization adsorbed FITC-collagen (a sign impaired remodeling), complemented sensitivity proteases. latter was two ways: measuring directly degradation attached quantifying proteolysis reduction upon exogenous addition collagenase cell-free system. mechanism observed effects unclear, although differential scanning calorimetry presence weak changes collagen. All this led us conclude reason ADMSCs early-glycated complementary sequences integrins, certainly affects mechanical remodeling collagenolytic activity, together small thermal transition profile, undoubtedly indicate some internal molecule occurring even at stage glycation, turn contributes activity.

Язык: Английский

Adhesion of Mesenchymal Stem Cells to Glycated Collagen—Comparative Analysis of Dynamic and Static Conditions

Polymers, Год журнала: 2025, Номер 17(6), С. 821 - 821

Опубликована: Март 20, 2025

Understanding mesenchymal stem cell (MSC) behavior on glycated collagen is crucial for advancing regenerative medicine and understanding pathological mechanisms in diseases such as diabetes, cancer, aging. While previous research has demonstrated reduced MSC interaction with under static conditions due to disrupted integrin signaling, these studies did not accurately replicate the dynamic mechanical environment that MSCs encounter vivo. Here we present a comprehensive investigation comparing adipose-derived (ADMSC) both flow adhesion, revealing unexpected temporal dynamics challenging existing paradigms of cell–matrix interactions. Using sophisticated microfluidic BioFlux system combined traditional adhesion assays, examined ADMSC interactions native 1-day (GL1), 5-day (GL5) samples. Under conditions, remarkably rapid attachment—within 3–5 min—contrasting sharply classical 2 h incubation protocol. This was particularly enhanced collagen, though subsequent testing revealed significantly weaker strength shear stress compared collagen. Static also showed distinct pattern: increased samples within first 30 min, followed by progressive decrease compromised spreading over longer periods. Atomic force microscopy (AFM) analysis significant changes surface properties upon glycation. These included substantial reduction negative charge (from ~800 600 mV), altered roughness patterns (Rrms varying from 3.0 ± 0.4 nm 7.70 0.6 GL5), decreased elasticity (Young’s modulus dropping 34.8 5.4 MPa 2.07 0.3 GL5). physical alterations appear facilitate initial attachment while potentially compromising long-term stable through integrin-mediated mechanisms. study provides novel insights into complex previously unknown models The findings suggest need revised approaches tissue engineering medicine, where prevalent.

Язык: Английский

Special Issue: The Role of Extracellular Matrix Proteins in Pathogenesis

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13367 - 13367

Опубликована: Дек. 13, 2024

The extracellular matrix (ECM) serves as a complex network that regulates cellular behavior and maintains tissue architecture [...]

Язык: Английский