Early Stages of Ex Vivo Collagen Glycation Disrupt the Cellular Interaction and Its Remodelling by Mesenchymal Stem Cells—Morphological and Biochemical Evidence
Опубликована: Фев. 26, 2024
Mesenchymal
stem
cells
(MSCs),
whose
main
function
is
tissue
repair,
use
collagen
to
restore
the
structural
integrity
of
damaged
tissue,
maintaining
its
organization
through
concomitant
remodelling.
The
non-enzymatic
glycation
likely
compromise
communication
with
MSC,
which
indeed
underlies
various
pathological
conditions
such
as
late
complications
diabetes
and
aging.
However,
data
on
effect
more
early
stages
MSC
interaction
are
lacking.
This
study
focused
fate
in
vitro
glycated
rat
tail
(RTC)
contact
MSCs
after
pre-exposure
glucose
for
1
or
5
days.
Using
human
adipose
tissue-derived
(ADMSCs),
we
showed
that
their
significantly
altered,
manifested
morphologically
by
reduced
cell
spreading,
less
formation
focal
adhesions,
weaker
development
actin
cytoskeleton,
further
confirmed
ImageJ
morphometric
analysis.
suggests
a
poorer
recognition
integrin
receptors,
possibly
due
steric
hindrance
binding
sites.
These
morphological
events
were
also
accompanied
greatly
fibril-like
reorganization
adsorbed
FITC-collagen
(a
sign
impaired
remodeling),
complemented
sensitivity
proteases.
latter
was
two
ways:
measuring
directly
degradation
attached
quantifying
proteolysis
reduction
upon
exogenous
addition
collagenase
cell-free
system.
mechanism
observed
effects
unclear,
although
differential
scanning
calorimetry
presence
weak
changes
collagen.
All
this
led
us
conclude
reason
ADMSCs
early-glycated
complementary
sequences
integrins,
certainly
affects
mechanical
remodeling
collagenolytic
activity,
together
small
thermal
transition
profile,
undoubtedly
indicate
some
internal
molecule
occurring
even
at
stage
glycation,
turn
contributes
activity.
Язык: Английский
Adhesion of Mesenchymal Stem Cells to Glycated Collagen—Comparative Analysis of Dynamic and Static Conditions
Polymers,
Год журнала:
2025,
Номер
17(6), С. 821 - 821
Опубликована: Март 20, 2025
Understanding
mesenchymal
stem
cell
(MSC)
behavior
on
glycated
collagen
is
crucial
for
advancing
regenerative
medicine
and
understanding
pathological
mechanisms
in
diseases
such
as
diabetes,
cancer,
aging.
While
previous
research
has
demonstrated
reduced
MSC
interaction
with
under
static
conditions
due
to
disrupted
integrin
signaling,
these
studies
did
not
accurately
replicate
the
dynamic
mechanical
environment
that
MSCs
encounter
vivo.
Here
we
present
a
comprehensive
investigation
comparing
adipose-derived
(ADMSC)
both
flow
adhesion,
revealing
unexpected
temporal
dynamics
challenging
existing
paradigms
of
cell–matrix
interactions.
Using
sophisticated
microfluidic
BioFlux
system
combined
traditional
adhesion
assays,
examined
ADMSC
interactions
native
1-day
(GL1),
5-day
(GL5)
samples.
Under
conditions,
remarkably
rapid
attachment—within
3–5
min—contrasting
sharply
classical
2
h
incubation
protocol.
This
was
particularly
enhanced
collagen,
though
subsequent
testing
revealed
significantly
weaker
strength
shear
stress
compared
collagen.
Static
also
showed
distinct
pattern:
increased
samples
within
first
30
min,
followed
by
progressive
decrease
compromised
spreading
over
longer
periods.
Atomic
force
microscopy
(AFM)
analysis
significant
changes
surface
properties
upon
glycation.
These
included
substantial
reduction
negative
charge
(from
~800
600
mV),
altered
roughness
patterns
(Rrms
varying
from
3.0
±
0.4
nm
7.70
0.6
GL5),
decreased
elasticity
(Young’s
modulus
dropping
34.8
5.4
MPa
2.07
0.3
GL5).
physical
alterations
appear
facilitate
initial
attachment
while
potentially
compromising
long-term
stable
through
integrin-mediated
mechanisms.
study
provides
novel
insights
into
complex
previously
unknown
models
The
findings
suggest
need
revised
approaches
tissue
engineering
medicine,
where
prevalent.
Язык: Английский
Special Issue: The Role of Extracellular Matrix Proteins in Pathogenesis
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(24), С. 13367 - 13367
Опубликована: Дек. 13, 2024
The
extracellular
matrix
(ECM)
serves
as
a
complex
network
that
regulates
cellular
behavior
and
maintains
tissue
architecture
[...]
Язык: Английский