Multi-Omics Analysis in Mouse Primary Cortical Neurons Reveals Complex Positive and Negative Biological Interactions Between Constituent Compounds of Centella asiatica DOI Creative Commons
Steven R. Chamberlin, Jonathan A. Zweig,

Cody J. Neff

и другие.

Pharmaceuticals, Год журнала: 2024, Номер 18(1), С. 19 - 19

Опубликована: Дек. 27, 2024

Background: A water extract of the Ayurvedic plant Centella asiatica (L.) Urban, family Apiaceae (CAW), improves cognitive function in mouse models aging and Alzheimer’s disease affects dendritic arborization, mitochondrial activity, oxidative stress primary neurons. Triterpenes (TT) caffeoylquinic acids (CQA) are constituents associated with these bioactivities CAW, although little is known about how interactions between compounds contribute to plant’s therapeutic benefit. Methods: Mouse cortical neurons were treated CAW or equivalent concentrations four TT combined, eight CQA twelve combined (TTCQA). Treatment effects on cell transcriptome (18,491 genes) metabolome (192 metabolites) relative vehicle control evaluated using RNAseq metabolomic analyses, respectively. Results: Extensive differentially expressed genes (DEGs) seen all treatments, as well evidence compounds. Notably, many DEGs treatment not observed TTCQA condition, possibly suggesting reduced TT. Moreover, additional gene activity compared indicates presence that further modulate interactions. Weighted Gene Correlation Network Analysis (WGCNA) identified 4 co-expression modules altered by treatments extracellular matrix organization, fatty acid metabolism, cellular response stimuli, immune function. Compound interaction patterns at eigengene level modules. Interestingly, metabolomics analysis, saw highest number changes individual metabolites (20), followed (15), then (8), finally (3). WGCNA analysis found two significant eigenmetabolite differences for possible compound this level. Conclusions: Four expression metabolite types applied. This methodology demonstrated existence both negative positive TT, CQA,

Язык: Английский

Trends and Pitfalls in the Progress of Network Pharmacology Research on Natural Products DOI Creative Commons
Alexander Panossian

Pharmaceuticals, Год журнала: 2025, Номер 18(4), С. 538 - 538

Опубликована: Апрель 7, 2025

Herbs, used as food and a source of medicine for centuries, have been extensively studied over time their chemical pharmacological properties, with two main aims [...]

Язык: Английский

Процитировано

0

Adaptogens in Long-Lasting Brain Fatigue: An Insight from Systems Biology and Network Pharmacology DOI Creative Commons
Alexander Panossian,

Terrence Lemerond,

Thomas Efferth

и другие.

Pharmaceuticals, Год журнала: 2025, Номер 18(2), С. 261 - 261

Опубликована: Фев. 15, 2025

Long-lasting brain fatigue is a consequence of stroke or traumatic injury associated with emotional, psychological, and physical overload, distress in hypertension, atherosclerosis, viral infection, aging-related chronic low-grade inflammatory disorders. The pathogenesis linked to disrupted neurotransmission, the glutamate-glutamine cycle imbalance, glucose metabolism, ATP energy supply, which are multiple molecular targets signaling pathways neuroendocrine-immune blood circulation systems. Regeneration damaged tissue long-lasting multistage process, including spontaneously regulating hypothalamus-pituitary (HPA) axis-controlled anabolic-catabolic homeostasis recover harmonized sympathoadrenal system (SAS)-mediated function, deregulated gene expression rehabilitation. driving mechanism spontaneous recovery regeneration cross-talk mediators neuronal, microglia, immunocompetent, endothelial cells collectively involved neurogenesis angiogenesis, plant adaptogens can target. Adaptogens small molecules origin that increase adaptability organisms stress by interaction HPA axis SAS (neuroendocrine-immune cardiovascular complex), targeting adaptive GPCR pathways. Two major groups comprise (i) phenolic phenethyl phenylpropanoid derivatives (ii) tetracyclic pentacyclic glycosides, whose chemical structure be distinguished as related correspondingly monoamine neurotransmitters (epinephrine, norepinephrine, dopamine) steroid hormones (cortisol, testosterone, estradiol). In this narrative review, we discuss multitarget integrated pharmacological activity botanical ischemic stroke, fatigue; time-dependent dual response physiological regulatory systems support overload; (iii) dose-dependent reversal (hormetic) effect adaptogens. This review shows adaptogenic concept cannot reduced rectified various effects on selected specific modes action without estimating their interactions within networks complex that, turn, regulates other (cardiovascular, gastrointestinal, reproductive systems) due numerous intra- extracellular communications feedback regulations. These result polyvalent pleiotropic adaptogens, essential for characterizing distinct types botanicals. They trigger defense leads extension limits resilience inducing mental For first time, justifies potential particularly hybrid preparation (BHP) Arctic Root Ashwagandha, providing rationale use individuals experiencing fatigue. provided insight into future research network pharmacology preventing rehabilitating following trauma, infections.

Язык: Английский

Процитировано

0

Pain Relief, Functional Recovery, and Chondroprotective Effects of Angelica gigas Nakai in Osteoarthritis Due to Its Anti-Inflammatory Property: An In Vitro and In Vivo Study DOI Open Access
Hee-Geun Jo,

Chae Yun Baek,

Yeseul Hwang

и другие.

Nutrients, Год журнала: 2024, Номер 16(15), С. 2435 - 2435

Опубликована: Июль 26, 2024

Osteoarthritis (OA), characterized by chronic pain and joint degradation, is a progressive disease primarily induced age-related systemic inflammation.

Язык: Английский

Процитировано

3

Efficacy and Safety of Orally Administered East Asian Herbal Medicine Combined with Narrowband Ultraviolet B against Psoriasis: A Bayesian Network Meta-Analysis and Network Analysis DOI Open Access
Hee-Geun Jo, Hyehwa Kim, Eunhye Baek

и другие.

Nutrients, Год журнала: 2024, Номер 16(16), С. 2690 - 2690

Опубликована: Авг. 13, 2024

Psoriasis is a chronic, immune-mediated inflammatory skin disease with many complications and poor prognosis that imposes significant burden on individuals society. Narrowband ultraviolet B (NB-UVB) represents cost-effective non-drug therapeutic intervention for psoriasis. East Asian herbal medicine (EAHM) currently being investigated its potential as safe effective psoriasis treatment. Consequently, it has the to be employed combination therapy NB-UVB. The objective was ascertain efficacy safety of EAHM NB-UVB identify important drugs further research. In this study, randomized controlled trials (RCTs) were retrieved from ten databases in Korea, China, Japan. All statistical analyses conducted using R software version 4.3.0. primary outcomes Area Severity Index (PASI) incidence rate adverse events (AEs), while secondary hematologic markers Dermatology Life Quality (DLQI), which reflect pathology analysis 40 RCTs, including 3521 participants, demonstrated exhibited statistically superiority over monotherapy respect outcomes. Bayesian network meta-analysis revealed Investigator Presciption 3 Ziyin Liangxue Decoction consistent relative advantage each PASI-based metric. estimated influence ranking all individual herbs according PageRank centrality. findings study suggest EAHMs co-administered may provide additional safety-related benefits patients However, quality evidence still low, high-quality are needed reach more definitive conclusions.

Язык: Английский

Процитировано

1

Multi-Omics Analysis in Mouse Primary Cortical Neurons Reveals Complex Positive and Negative Biological Interactions Between Constituent Compounds of Centella asiatica DOI Creative Commons
Steven R. Chamberlin, Jonathan A. Zweig,

Cody J. Neff

и другие.

Pharmaceuticals, Год журнала: 2024, Номер 18(1), С. 19 - 19

Опубликована: Дек. 27, 2024

Background: A water extract of the Ayurvedic plant Centella asiatica (L.) Urban, family Apiaceae (CAW), improves cognitive function in mouse models aging and Alzheimer’s disease affects dendritic arborization, mitochondrial activity, oxidative stress primary neurons. Triterpenes (TT) caffeoylquinic acids (CQA) are constituents associated with these bioactivities CAW, although little is known about how interactions between compounds contribute to plant’s therapeutic benefit. Methods: Mouse cortical neurons were treated CAW or equivalent concentrations four TT combined, eight CQA twelve combined (TTCQA). Treatment effects on cell transcriptome (18,491 genes) metabolome (192 metabolites) relative vehicle control evaluated using RNAseq metabolomic analyses, respectively. Results: Extensive differentially expressed genes (DEGs) seen all treatments, as well evidence compounds. Notably, many DEGs treatment not observed TTCQA condition, possibly suggesting reduced TT. Moreover, additional gene activity compared indicates presence that further modulate interactions. Weighted Gene Correlation Network Analysis (WGCNA) identified 4 co-expression modules altered by treatments extracellular matrix organization, fatty acid metabolism, cellular response stimuli, immune function. Compound interaction patterns at eigengene level modules. Interestingly, metabolomics analysis, saw highest number changes individual metabolites (20), followed (15), then (8), finally (3). WGCNA analysis found two significant eigenmetabolite differences for possible compound this level. Conclusions: Four expression metabolite types applied. This methodology demonstrated existence both negative positive TT, CQA,

Язык: Английский

Процитировано

0