Plasma Redox Balance in Advanced Maternal Age Pregnant Women and Effects of Plasma on Umbilical Cord Mesenchymal Stem Cells
Опубликована: Март 20, 2024
Pregnancy
at
advanced
maternal
age
(AMA)
is
a
condition
of
potential
risk
for
the
development
maternal-fetal
complications
with
possible
repercussions
even
in
long-term.
Here,
we
analyzed
changes
plasma
redox
balance
and
effects
on
human
umbilical
cord
mesenchymal
cells
(hUMSCs)
AMA
pregnant
women
(patients)
various
timing
pregnancy.
One
hundred
patients
twenty
younger
than
40
years
(controls)
were
recruited,
evaluated
timings
during
pregnancy
until
after
delivery.
Plasma
samples
used
to
measure
thiobarbituric
acid
reactive
substances
(TBARS),
glutathione
nitric
oxide
(NO).
In
addition,
was
stimulate
hUMSCs,
which
tested
cell
viability,
oxygen
species
(ROS)
NO
release.
The
obtained
results
showed
that
throughout
delivery
levels
lower
those
controls,
while
TBARS
higher.
Moreover,
reduced
viability
release,
increased
ROS
release
hUMSCs.
Our
highlighted
alterations
presence
potentially
harmful
circulating
factors
They
could
have
clinical
relevance
prevention
related
Язык: Английский
Extracellular Vesicles and Pregnancy-Related Hypertensive Disorders: A Descriptive Review on the Possible Implications “From Bench to Bedside”
Biology,
Год журнала:
2025,
Номер
14(3), С. 240 - 240
Опубликована: Фев. 27, 2025
Pregnancy
involves
extracellular
vesicles
(EVs)
through
mechanisms
that
are
poorly
understood
to
date.
Furthermore,
it
is
not
surprising
EVs
may
also
be
involved
in
the
pathophysiology
of
pre-eclampsia
(PE)
and
gestational
hypertension,
two
clinical
conditions
with
high
morbidity
mortality,
given
their
capacity
mediate
intracellular
communications
regulate
inflammation
angiogenesis.
We
searched
major
online
scientific
search
engines
(PubMed,
Google
Scholar,
Scopus,
WES,
Embase,
etc.)
using
terms
“Preeclampsia”,
“Pregnancy”,
“Hypertension”,
“Pregnancy-related
hypertension”,
“Extracellular
vesicles”,
“Biomarkers”,
“Gestation”
AND
“Obstetrics”.
Finding
potential
early
biomarkers
risk
or
illness
progression
would
essential
for
optimum
care
expectant
mothers
aforementioned
conditions.
Nevertheless,
none
various
screening
assays
have
been
discovered
recently
shown
predictive
values.
The
analysis
peripheral
blood
starting
from
first
trimester
pregnancy
hold
great
promise
possible
correlation
hypertension
problems
represent
a
marker
stages
disease.
use
novel
therapeutic
approach
management
illnesses,
as
well.
In
order
define
EVs’
function
physiopathology
pregnancy-associated
PE,
well
tools,
we
compiled
most
recent
data
this
review.
Язык: Английский
Characterization of Circulating Vesicles of Complicated and Uncomplicated Systemic Sclerosis Patients and Their Role in Vascular Dysfunction
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(6), С. 2380 - 2380
Опубликована: Март 7, 2025
Extracellular
vesicles
(EVs)
could
be
involved
in
the
onset
of
systemic
sclerosis
(SSc)
through
modulation
vascular
function.
Anyway,
available
data
are
contradictory,
and
further
investigation
would
necessary
to
clarify
this
aspect.
Here,
we
characterized
circulating
EVs
isolated
from
SSc
patients
evaluated
their
effects
on
human
endothelial
cells
(HUVECs)
smooth
muscle
cells.
In
13
complicated
27
uncomplicated
five
healthy
controls
(HCs),
analyzed
size,
concentration,
surface
marker
expression.
addition,
were
used
stimulate
HUVECs,
cell
viability,
mitochondrial
membrane
potential,
nitric
oxide
(NO)
reactive
oxygen
species
(MitoROS)
release.
cells,
calcium
movement
examined.
The
results
showed
that
expressed
markers
T-lymphocyte/platelet/endothelial
origin
larger
more
concentrated
than
those
HCs.
reduced
viability
potential
increased
NO
MitoROS
release
HUVECs
intracellular
conclusion,
found
a
specific
pattern
for
patients,
which
have
pathogenic
role
direct
actions
Язык: Английский
Chromogranin B Protects Human Umbilical Endothelial Cells against Oxidative Stress
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(19), С. 10296 - 10296
Опубликована: Сен. 25, 2024
Chromogranin
B
(CgB)
is
involved
in
the
control
of
cardiovascular
system
through
regulation
catecholamine
release.
Whether
CgB
can
exert
direct
actions
on
endothelium
has
not
yet
been
clarified.
Here,
we
aimed
to
investigate
effects
cell
viability,
mitochondrial
membrane
potential,
reactive
oxygen
species
(ROS),
glutathione
(GSH),
nitric
oxide
(NO)
release,
and
cytosolic
calcium
concentration
([Ca2+]c)
human
vascular
endothelial
cells
(HUVECs)
cultured
under
both
physiological
peroxidative
conditions.
In
HUVECs,
experiments
were
conducted
establish
proper
timing
stimulation.
Thereafter,
specific
assays
used
evaluate
response
HUVECs
physiologic
or
oxidative
stress
conditions
presence
absence
β-adrenergic
receptor
agonists
antagonists
intracellular
pathways
blockers.
Analysis
NO
release
revealed
that
was
able
cause
increased
Additionally,
same
analyses
performed
with
H2O2,
showed
protective
exerted
by
CgB,
which
also
counteract
ROS
maintain
GSH
levels.
Furthermore,
played
a
dual
role
[Ca2+]c
depending
culturing
conclusion,
our
data
provide
new
information
about
function
highlight
its
potential
as
agent
against
conditions,
such
those
found
diseases.
Язык: Английский
Sudden Intrauterine Unexplained Death (SIUD) and Oxidative Stress: Placental Immunohistochemical Markers
Cells,
Год журнала:
2024,
Номер
13(16), С. 1347 - 1347
Опубликована: Авг. 13, 2024
Background:
Intrauterine
fetal
death
and
perinatal
represent
one
of
the
most
relevant
medical
scientific
problems
since,
in
many
cases,
even
after
extensive
investigation,
causes
remain
unknown.
The
considerable
increase
legal
litigation
obstetrical
field
that
has
witnessed
recent
years,
especially
cases
stillborn
births,
simultaneously
involved
figure
forensic
pathologist
research
aimed
at
clarifying
pathophysiological
processes
underlying
stillbirth.
Methods:
our
study
aims
to
analyze
sudden
intrauterine
unexplained
syndrome
(SIUD)
evaluate
role
oxidative
stress
complex
pathogenetic
process
In
particular,
immunohistochemical
expression
specific
markers
(NOX2,
NT,
iNOS,
8-HODG,
IL-6)
was
evaluated
tissue
samples
placentas
SIUDs
belonging
case
series
(20
cases),
collected
from
autopsy
University
Ferrara
Politecnica
delle
Marche
between
2017
2023.
Results:
demonstrated
involvement
deaths
placenta
examined.
SIUD,
expressed
were
NOX2
8-HODG.
Conclusions:
contributes
investigating
modulating
different
pathways
tissues.
Язык: Английский