A Structural Proteome Screen Identifies Protein Mimicry in Host-Microbe Systems DOI Open Access
Gabriel Penunuri,

Pingting Wang,

Russell Corbett‐Detig

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 13, 2024

Summary Host-microbe systems are evolutionary niches that produce coevolved biological interactions and a key component of global health. However, these have historically been difficult field research due to their experimental intractability. Impactful advances in health will be obtained by leveraging silico screens identify genes involved mediating interspecific interactions. These predictions progress our understanding lay the groundwork for future vitro vivo experiments bioengineering projects. A driver host-manipulation intracellular survival utilized host-associated microbes is molecular mimicry, critical mechanism can occur at any level from DNA protein structures. We applied structure prediction alignment tools explore bacterial structural proteomes examples mimicry. By Legionella pneumophila proteome its many known mimics, we developed validated screen virtually host-microbe system uncover signals mimics represent candidate proteins mediate host microbial proteomes. successfully this other with demonstrated effects on health, Helicobacter pylori Wolbachia , identifying mimic candidates each proteome. discuss roles may play important -induced phenotypes show Wobachia infection partially rescue loss one factors. This work demonstrates how genome-wide offers an opportunity functionally systems.

Язык: Английский

Diverse Genetic Conflicts Mediated by Molecular Mimicry and Computational Approaches to Detect Them DOI Open Access
Shelbi L. Russell, Gabriel Penunuri,

Christopher Condon

и другие.

Опубликована: Март 28, 2024

In genetic conflicts, driver and killer elements achieve biased survival, replication, or transmission over sensitive targeted through a wide range of molecular mechanisms, including mimicry. Driving mechanisms manifest at all organismal levels, from the propagation individual genes, as demonstrated by transposable elements, to genomes, illustrated viruses, cell lineages, in cancer. Targeted genomes are vulnerable mimicry conserved motifs they use for their own signaling regulation. Mimicking these enables selfish element control core target processes, can occur sequence, structure, functional level. Molecular was first appreciated an important phenomenon more than twenty years ago. Modern genomics technologies, databases, machine learning approaches offer tremendous potential study distribution across conflicts nature. Here, we explore theoretical expectations between conflicting trends known outline how new examples be gleaned population genomic datasets. We discuss mimics involving short sequence-based gene duplications evolve convergently mutations. Whereas, processes that involve divergent domains fully-folded structures among horizontal transfer. These largely based on small number organisms should reevaluated general, phylogenetically independent framework. Currently, publicly available databases mined genotypes driving non-mendelian inheritance patterns, epistatic interactions, convergent protein structures. A subset may mimics. propose detecting conflict

Язык: Английский

Процитировано

2

A Structural Proteome Screen Identifies Protein Mimicry in Host-Microbe Systems DOI Open Access
Gabriel Penunuri,

Pingting Wang,

Russell Corbett‐Detig

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 13, 2024

Summary Host-microbe systems are evolutionary niches that produce coevolved biological interactions and a key component of global health. However, these have historically been difficult field research due to their experimental intractability. Impactful advances in health will be obtained by leveraging silico screens identify genes involved mediating interspecific interactions. These predictions progress our understanding lay the groundwork for future vitro vivo experiments bioengineering projects. A driver host-manipulation intracellular survival utilized host-associated microbes is molecular mimicry, critical mechanism can occur at any level from DNA protein structures. We applied structure prediction alignment tools explore bacterial structural proteomes examples mimicry. By Legionella pneumophila proteome its many known mimics, we developed validated screen virtually host-microbe system uncover signals mimics represent candidate proteins mediate host microbial proteomes. successfully this other with demonstrated effects on health, Helicobacter pylori Wolbachia , identifying mimic candidates each proteome. discuss roles may play important -induced phenotypes show Wobachia infection partially rescue loss one factors. This work demonstrates how genome-wide offers an opportunity functionally systems.

Язык: Английский

Процитировано

0