Опубликована: Май 13, 2024

Glioblastoma (GB) is the most aggressive and prevalent glioma within Central Nervous System. GB exhibit a dismal 5-year survival rate (~6%) due to unfavorable prognosis lack of viable treatment options. Therefore, novel therapies with plant-derived compounds emerge as very promising. This study aims investigate antitumor activity 7α-acetoxy-6β-hydroxyroyleanone (Roy) in cell lines. Roy was isolated from Plectranthus hadiensis Schweinf. its mechanism assessed panel five lines mimic tumor heterogeneity. Metabolic evaluated by Alamar Blue® assay, while death, cycle regulation, mitochondrial membrane potential, activated caspase-3 were flow cytometry. mRNA levels target genes measured qPCR protein expression Western blotting. Cell uptake impact on morphology confocal microscopy. induced G2/M arrest, fragmentation, apoptosis inhibiting anti-apoptotic proteins increasing concentration 6-9 fold lower than those temozolomide, first-line treatment. demonstrated robust against cells, without affecting non tumoral cells (microglia astrocytes), being promising natural lead compound for development chemotherapeutic approaches.

Язык: Английский