ClpL Chaperone as a Possible Component of the Disaggregase Activity of Limosilactobacillus fermentum U-21
Опубликована: Май 27, 2024
Strain
L.
fermentum
U-21
secretes
chaperones
into
the
medium
and
it
is
considered
as
candidate
for
development
of
drugs,
so-called
disaggregases,
Parkinson’s
disease
therapy.
This
study
devoted
to
characterization
secreted
protein
encoded
by
C0965_000195
locus.
Analysis
sequence
predicted
structure
allows
us
attribute
ClpL,
homologs
which
are
known
be
chaperones.
The
chaperone
activity
ClpL
was
evaluated
in
vivo
refolding
differing
thermostability
luciferases
from
Aliivibrio
fischeri
Photorhabdus
luminescens
Escherichia
coli
cells.
It
observed
that
clpL
can
compensate
deficiency
clpB
gene
enhance
ability
refold
thermodenatured
proteins
clpB-
In
vitro
experiments
have
demonstrated
a
spent
culture
containing
cells,
including
prevent
thermodenaturation
partially.We
suggest
strain,
exhibits
disaggregase
properties
against
aggregating
proteins,
may
one
clue
components
play
role
pharmabiotic
this
strain.
Язык: Английский
Levilactobacillus brevis 47f: Bioadaptation to Low Doses of Xenobiotics in Aquaculture
Biology,
Год журнала:
2024,
Номер
13(11), С. 925 - 925
Опубликована: Ноя. 14, 2024
Agricultural
and
industrial
activities
are
increasing
pollution
of
water
bodies
with
low
doses
xenobiotics
that
have
detrimental
effects
on
aquaculture.
The
aim
this
work
was
to
determine
the
possibility
using
Levilactobacillus
brevis
47f
culture
in
fish
aquaculture
under
influence
as
an
adaptogen.
An
increase
survival
Danio
rerio
individuals
exposed
xenobiotic
bisphenol
A
solution
fed
L.
shown
compared
control
groups
and,
at
same
time,
cytokine
profile
intestinal
tissues
also
investigated.
Analysis
differential
gene
expression
grown
action
high
concentrations
showed
changes
mRNA
levels
a
number
genes,
including
genes
various
transport
proteins,
involved
fatty
acid
synthesis,
transcriptional
regulators,
arabinose
operon,
oppA
gene.
identification
proteins
from
polyacrylamide
gel
by
mass
spectrometry
revealed
L-arabinose
isomerase,
Clp
chaperone
subunit,
ATP
synthase
subunits,
pentose
phosphate
pathway
glycolysis
enzyme
which
likely
part
strain’s
anti-stress
response,
but
probably
do
not
affect
its
adaptogenic
activity
toward
rerio.
Язык: Английский