Lycium Barbarum Glycopeptide Alleviates Retinal Inflammation by Suppressing Microglial M1 Polarization via NF-κB/MAPK Pathways DOI Open Access
Yiwen Ou, Yinhua Huang,

Xu Yang

и другие.

Опубликована: Май 29, 2024

Microglia-related retinal inflammation is associated with a variety of diseases. Lycium barbarum Glycopeptide (LBGP) the main active component polysaccharide (LBP) isolated from barbarum, which possesses antioxidation, neuroprotection, and immunoregulation. However, whether LBGP has beneficial effects on LPS/IFN-γ (L/I) induced microglial activation its underlying mechanism remains to be elucidated. In this study, we observed anti-inflammatory properties in microglia activated by LPS IFN-γ (L/I), examining both primary BV2 cells. Cells were pretreated for 2h before stimulated 24h. The dosages cells evaluated using CCK-8 assay. morphological changes an optical microscope. expression cytokines inflammatory mediators measured Griess assay, ELISA, qPCR, immunofluorescence. Western blotting was used assess MAPK, NF-κB signal transducer. pre-treatment significantly reversed L/I-induced alterations area, perimeter, Feret's diameter, roundness. alleviated switched M1 pro-inflammatory phenotype M2 downregulating markers (iNOS, IL-1β, IL-6, COX2, CD16, CD86) upregulating (arginase 1 CD206). Furthermore, attenuated MAPK pathways L/I-activated summary, it suggested that pretreatment regulating microglial(M1/M2) polarization via signaling pathways.

Язык: Английский

Lycium Barbarum Glycopeptide Alleviates Retinal Inflammation by Suppressing Microglial M1 Polarization via NF-κB/MAPK Pathways DOI Open Access
Yiwen Ou, Yinhua Huang,

Xu Yang

и другие.

Опубликована: Май 29, 2024

Microglia-related retinal inflammation is associated with a variety of diseases. Lycium barbarum Glycopeptide (LBGP) the main active component polysaccharide (LBP) isolated from barbarum, which possesses antioxidation, neuroprotection, and immunoregulation. However, whether LBGP has beneficial effects on LPS/IFN-γ (L/I) induced microglial activation its underlying mechanism remains to be elucidated. In this study, we observed anti-inflammatory properties in microglia activated by LPS IFN-γ (L/I), examining both primary BV2 cells. Cells were pretreated for 2h before stimulated 24h. The dosages cells evaluated using CCK-8 assay. morphological changes an optical microscope. expression cytokines inflammatory mediators measured Griess assay, ELISA, qPCR, immunofluorescence. Western blotting was used assess MAPK, NF-κB signal transducer. pre-treatment significantly reversed L/I-induced alterations area, perimeter, Feret's diameter, roundness. alleviated switched M1 pro-inflammatory phenotype M2 downregulating markers (iNOS, IL-1β, IL-6, COX2, CD16, CD86) upregulating (arginase 1 CD206). Furthermore, attenuated MAPK pathways L/I-activated summary, it suggested that pretreatment regulating microglial(M1/M2) polarization via signaling pathways.

Язык: Английский

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