Characterizing A21: Natural Cyanobacteria-Based Consortium with Potential for Steroid Bioremediation in Wastewater Treatment DOI Open Access
Govinda Guevara,

Jamileth Stefania Espinoza Solorzano,

Marta Ramírez

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 13018 - 13018

Опубликована: Дек. 4, 2024

Microalga-bacteria consortia are increasingly recognized for their effectiveness in wastewater treatment, leveraging the metabolic synergy between microalgae and bacteria to enhance nutrient removal overall treatment efficiency. These systems offer a sustainable approach addressing pollutants such as nitrogen phosphorus. However, potential removing specific contaminants like steroid hormones is less explored. In this study, natural microbial consortium, A21, has been characterized isolated from primary sewage Madrid its bioremediation of hormone effluents evaluated. The A21 consortium includes Alphaproteobacteria genera

Язык: Английский

Bioconvection in Microalgae: Review of Mathematical Models DOI Creative Commons

Laura Barsanti,

Lorenzo Birindelli,

Angelo Di Garbo

и другие.

Applied Sciences, Год журнала: 2025, Номер 15(5), С. 2708 - 2708

Опубликована: Март 3, 2025

Bioconvection can be defined as an aggregation pattern-generating phenomenon characterized by the collective behavior of swimming microorganisms in response to external influences and self-induced fluid flow, it has attracted significant interest study microalgae. This review aims provide a comprehensive examination this concentrated population suspended microalgae definable nonlinear dynamical systems (i.e., generators spatial temporal patterns), describing clarifying underlying mechanisms, ecological implications, possible biotechnological applications different fields for improvement bioreactor design biomass cultivation, wastewater treatment, or bioremediation. The most influential models used capture complexity bioconvection are perused order explore intricate interplay internal (biological, physical, environmental) governing pattern formation dynamics alongside recent advancements modeling techniques experimental approaches.

Язык: Английский

Процитировано

1

The Microalgae <em>Chlamydomonas </em>for Bioremediation and Bioproduct Production DOI Open Access
Carmen M. Bellido-Pedraza, María J. Torres, Ángel Llamas

и другие.

Опубликована: Июнь 3, 2024

The extensive metabolic diversity of microalgae, coupled with their rapid growth rates and cost-effective production, position these organisms as highly promising resources for a wide range biotechnological applications. These characteristics allow microalgae to address crucial needs in both the agricultural, medical, industrial sectors. Microalgae are proving be val-uable various fields, including remediation diverse wastewater types, production biofuels biofertilizers, extraction products from biomass. For decades, microalga Chlamydomonas has been widely used fundamental research model organism areas such photosynthesis, respiration, sulfur phosphorus metabolism, nitrogen flagella synthesis, among others. However, recent years, potential tool bioremediation, biofertilization, biomass, bio-products increasingly recognized. Bioremediation using presents significant sustainable reduction contaminants fa-cilitates resource recovery valorization microalgal offering important economic benefits. also established itself platform va-riety biotechnologically interesting products, different types biofuels, high-value-added products. aim this review is achieve comprehensive understanding aspects, explore interrelationship, which would offer environmental advantages.

Язык: Английский

Процитировано

7

Applications of the Microalgae Chlamydomonas and Its Bacterial Consortia in Detoxification and Bioproduction DOI Creative Commons
María J. Torres, Carmen M. Bellido-Pedraza, Ángel Llamas

и другие.

Life, Год журнала: 2024, Номер 14(8), С. 940 - 940

Опубликована: Июль 27, 2024

The wide metabolic diversity of microalgae, their fast growth rates, and low-cost production make these organisms highly promising resources for a variety biotechnological applications, addressing critical needs in industry, agriculture, medicine. use microalgae consortia with bacteria is proving valuable several areas biotechnology, including the treatment various types wastewater, biofertilizers, extraction products from biomass. monoculture microalga Chlamydomonas has been prominent research model many years extensively used study photosynthesis, sulphur phosphorus metabolism, nitrogen respiration, flagellar synthesis, among others. Recent increasingly recognised potential Chlamydomonas-bacteria as tool applications. detoxification wastewater using its bacterial offers significant sustainable reduction contaminants, while facilitating resource recovery valorisation microalgal biofertilizers can offer benefits, such increasing crop yields, protecting crops, maintaining soil fertility stability, contributing to CO2 mitigation, agricultural practises. Chlamydomonas-bacterial play an important role high-value products, particularly biofuels enhancement H2 production. This review aims provide comprehensive understanding identify current applications propose new development directions maximise potential.

Язык: Английский

Процитировано

3

Bioremediation of Synthetic Wastewater with Contaminants of Emerging Concern by Nannochloropsis sp. and Lipid Production: A Circular Approach DOI Creative Commons
B. C. dos Santos, Juliana Araújo, BEATRIZ MACIEL DE CARVALHO

и другие.

Bioengineering, Год журнала: 2025, Номер 12(3), С. 246 - 246

Опубликована: Фев. 28, 2025

Contaminants of emerging concern (CECs) pose a potential risk to human and environmental health. Microalgae bioremediation is promising approach for transforming or removing contaminants from the environment, while contributing circular economy. In this study, Nannochloropsis sp. was effectively used simultaneous removal six CECs: paracetamol, ibuprofen, imidacloprid, methylparaben bisphenol A at 10 µg mL−1 triclosan 0.5 synthetic wastewater, which were able survive under such concentrations, higher than those commonly found in environment (up 2.82 methylparaben). High efficiencies reached (100%) (93 ± 2%), paracetamol 30 1%, 64 2% 49 5% removed, respectively. Subsequently, lipids extracted, FAME profile characterised using GS-MS. The main fatty acids identified after hexadecadienoic acid isomers (C16:2), palmitic (C16), linoleic (C18:2) γ-linolenic (C18:3). absence oleic stearic noticed, suggesting an alteration lipidic due contaminant exposure. By exploring quantification future work, applications extracted can be explored, further demonstrating feasibility process.

Язык: Английский

Процитировано

0

Enhancing Biomass and Lipid Production in Messastrum gracile Using Inorganic Carbon Substrates and Alternative Solvents for Lipid Extraction DOI Creative Commons
Wanida Pan-utai, Soisuda Pornpukdeewattana,

Wilasinee Inrung

и другие.

Life, Год журнала: 2025, Номер 15(3), С. 407 - 407

Опубликована: Март 5, 2025

Microalgae show promise as a biomass and bioproduct for applications in various industries. The cultivation of microalgae plays crucial role optimizing yield accumulation. provision inorganic carbon substrates substantially enhances microalgal growth lipid biosynthesis, resulting marked increases the production biofuels other bioproducts. This study examined accumulation Messastrum gracile IFRPD 1061 under stress conditions, previously unreported. M. was subjected to varying conditions substrates, 1–3 g·L−1 sodium carbonate bicarbonate concentration, enhance Optimal productivity levels were observed with addition 3 1 lipids, productivities 392.64 53.57 mg·L−1·d−1, respectively. Results underlined effectiveness sources stimulating enhancing high-value products. extraction lipids from freeze-dried demonstrated optimal using methanol/hexane solvents compared control experiments. Lipid yields 42.18% 46.81% oven-dried biomass, Lipids extracted methanol/hexane/chloroform indicated potential solvent dry an ultrasonic-assisted technique. contributes valuable insights into maximizing biofuel microalgae, highlighting A. gracilis promising candidate industrial applications.

Язык: Английский

Процитировано

0

Synthetic Biology Strategies and Tools to Modulate Photosynthesis in Microbes DOI Open Access

Shujin Fu,

Kaiyu Ma, Xinyu Song

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3116 - 3116

Опубликована: Март 28, 2025

The utilization of photosynthetic microbes, such as cyanobacteria and microalgae, offers sustainable solutions to addressing global resource shortages pollution. While these microorganisms have demonstrated significant potential in biomanufacturing, their industrial application is limited by suboptimal efficiency. Synthetic biology integrates molecular biology, systems engineering principles provide a powerful tool for elucidating mechanisms rationally optimizing platforms. This review summarizes recent advancements regulating photosynthesis microalgae via synthetic focusing on strategies enhance light energy absorption, optimize electron transport chains, improve carbon assimilation. Furthermore, we discuss key challenges translating genetic modifications large-scale bioproduction, highlighting specific bottlenecks strain stability, metabolic burden, process scalability. Finally, propose solutions, AI-assisted engineering, microbial consortia, next-generation photobioreactor designs, overcome limitations. Overall, while holds great promise enhancing efficiency further research needed refine develop scalable production systems.

Язык: Английский

Процитировано

0

Insertional Mutagenesis as a Strategy to Open New Paths in Microalgal Molybdenum and Nitrate Homeostasis DOI Creative Commons
Esperanza Leon-Miranda, Manuel Tejada‐Jiménez, Ángel Llamas

и другие.

Current Issues in Molecular Biology, Год журнала: 2025, Номер 47(6), С. 396 - 396

Опубликована: Май 26, 2025

Molybdenum (Mo) is a vital micronutrient for nearly all living organisms, serving as cofactor molybdoenzymes that catalyze essential redox reactions in nitrogen metabolism. Among these enzymes, nitrate reductase plays crucial role assimilation. Maintaining Mo homeostasis—including uptake, storage, and utilization—is critical to avoid both deficiency toxicity. Our research focuses on uncovering novel molecular components involved homeostasis, particularly connection with assimilation, using Chlamydomonas reinhardtii, model green microalga. To achieve this, we generated more than 5000 transformants through insertional mutagenesis paromomycin resistance cassette (AphVIII) screened them altered growth under different concentrations. We identified four strains showing patterns when source or exhibiting increased sensitivity Mo. The genomic alterations were identified. Notably, Mo-resistant Mo-sensitive transformant had disruptions the genes encoded ABC-type transport proteins, indicating potential proteins transport. Additionally, two unable grow nitrate. One of mutation CNX7, gene biosynthesis, while other BAT1, an amino acid transporter. BAT1 mutant represents interesting case study, this has not previously been associated These findings enhance our understanding homeostasis mechanisms open new paths engineering microalgae improved

Язык: Английский

Процитировано

0

Characterizing A21: Natural Cyanobacteria-Based Consortium with Potential for Steroid Bioremediation in Wastewater Treatment DOI Open Access
Govinda Guevara,

Jamileth Stefania Espinoza Solorzano,

Marta Ramírez

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 13018 - 13018

Опубликована: Дек. 4, 2024

Microalga-bacteria consortia are increasingly recognized for their effectiveness in wastewater treatment, leveraging the metabolic synergy between microalgae and bacteria to enhance nutrient removal overall treatment efficiency. These systems offer a sustainable approach addressing pollutants such as nitrogen phosphorus. However, potential removing specific contaminants like steroid hormones is less explored. In this study, natural microbial consortium, A21, has been characterized isolated from primary sewage Madrid its bioremediation of hormone effluents evaluated. The A21 consortium includes Alphaproteobacteria genera

Язык: Английский

Процитировано

2